Differential Expression Of Pro-inflammatory Cytokines And A Pro-apoptotic Protein In Pelvic Ileal Pouches For Ulcerative Colitis And Familial Adenomatous Polyposis

dc.creatorLeal R.F.
dc.creatorCoy C.S.R.
dc.creatorAyrizono M.L.S.
dc.creatorFagundes J.J.
dc.creatorMilanski M.
dc.creatorSaad M.J.
dc.creatorVelloso L.A.
dc.creatorGoes J.R.N.
dc.date2008
dc.date2015-06-30T19:35:55Z
dc.date2015-11-26T14:46:01Z
dc.date2015-06-30T19:35:55Z
dc.date2015-11-26T14:46:01Z
dc.date.accessioned2018-03-28T21:55:28Z
dc.date.available2018-03-28T21:55:28Z
dc.identifier
dc.identifierTechniques In Coloproctology. , v. 12, n. 1, p. 33 - 38, 2008.
dc.identifier11236337
dc.identifier10.1007/s10151-008-0395-9
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-44649101390&partnerID=40&md5=be68d23c5d61dc5e1913102d8d3bc4be
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/106798
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/106798
dc.identifier2-s2.0-44649101390
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1252670
dc.descriptionBackground: Pouchitis after total rectocolectomy is among the most common complications of patients with ulcerative colitis (UC). However, its frequency is quite rare in patients with familial adenomatous polyposis (FAP). We evaluated the inflammatory and pro-apoptotic activity in endoscopically normal mucosa of the ileal pouch in patients with UC and FAP. Methods: Twenty patients (10 with UC and 10 with FAP) with "J" pouch after total proctocolectomy were studied as were 10 normal controls. Biopsies were obtained from the mucosa of the pouch of UC and FAP patients and from the normal ileum of controls. The expression levels of TNF-α, IL-1β, IL-6, IL-8 and phospho-BAD were determined by immunoblotting. Activated NFκB was evaluated by immuno-precipitation and immunoblotting for IkappaB kinase beta. Results: Patients with UC had higher levels of IL-1β, IL-6, IL-8 and TNF-α than patients with FAP. The level of TNF-α was higher in patients with UC than in patients with FAP; both patient groups had TNF-α levels higher than controls. Activation of NFκB was similar in all three groups. The expression of phospho-BAD was significantly lower in patients with FAP than in patients with UC. Conclusions: As compared with patients with FAP, patients with UC presented increased levels of some pro-inflammatory cytokines, even in the absence of clinical or endoscopic signs of pouchitis. Patients with FAP presented lower levels of pro-inflammatory proteins and of phospho-BAD. These findings may explain the higher rates of progression to pouchitis in UC patients, which could correlate with mucosal atrophy that occurs in inflamed tissue. © 2008 Springer-Verlag Italia.
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dc.languageen
dc.publisher
dc.relationTechniques in Coloproctology
dc.rightsfechado
dc.sourceScopus
dc.titleDifferential Expression Of Pro-inflammatory Cytokines And A Pro-apoptotic Protein In Pelvic Ileal Pouches For Ulcerative Colitis And Familial Adenomatous Polyposis
dc.typeArtículos de revistas


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