A flow injection hydride generation system with a metal furnace atomizer (Inconel 600® alloy) was employed for Bi and Se determination. The presented methods have linear ranges up to 200 and 500 μg L- 1 for Bi and Se, respectively, with good linearities (r2 = 0.9997 and 0.9974, respectively). The limits of quantification obtained according to IUPAC recommendations were 2.3 μg L- 1 for Bi and 6 μg L- 1 for Se, and the relative standard deviations (N = 6) based on Bi and Se analytical responses from real samples were 2.7% and 10%, respectively. Accuracy evaluations were based on certified materials such as SRM 361, SRM 363, and SRM 364 (steel alloys) for Bi, Mess-3 (marine sediment), SRM 397 (human hair), and Bio-Rad2 - 69042 (urine) for Se. Good agreements between the results were obtained at the 95% confidence level, according to the t-test. © 2008 Elsevier B.V. All rights reserved.638850855Sumida, T., Ikenoue, T., Hamada, K., Sabarudin, A., Oshima, M., Motomizu, S., On-line pre-concentration using dual mini-columns for the speciation of chromium(III) and chromium(VI) and its application to water samples as studied by inductively coupled plasma-atomic emission spectrometry (2005) Talanta, 68, pp. 388-393Figueiredo, E.C., Tarley, C.R.T., Kubota, L.T., Rath, S., Arruda, M.A.Z., On-line molecularly imprinted solid phase extraction for the selective spectrophotometric determination of catechol (2007) Microchem. J., 85, pp. 290-296Journet, E., Desboeufs, K.V., Sofikitis, A., Varrault, G., Colin, J.L., In situ speciation of trace Fe(II) and Fe(III) in atmospheric waters by the FZ method coupled to GF AAS analysis (2007) Int. J. Environ. Anal. Chem., 87, pp. 647-658Lavilla, I., González-Costas, J.M., Merino-Meroño, B., Campillo, N., Hernández-Córdoba, M., Determination of selenium species in infant formulas and dietetic supplements using liquid chromatography-hydride generation atomic fluorescence spectrometry (2007) Anal. Chim. Acta, 591, pp. 225-230Carrero, P., Gutiérrez, L., Rondón, C., Burguera, J.L., Burguera, M., Peña, Y.P., Flow injection determination of bismuth in urine by successive retention of Bi(III) and tetrahydroborate(III) on an anion-exchange resin and hydride generation atomic absorption spectrometry (2004) Talanta, 64, pp. 1309-1316Dědina, J., Matousek, T., Multiple microflame - a new approach to hydride atomization for atomic absorption spectrometry (2000) J. Anal. At. Spectrom., 15, pp. 301-304Xie, R.M., Johnson, W., Spayd, S., Hall, G.S., Buckley, B., Determination of total toxic arsenic species in human urine using hydride generation inductively coupled plasma mass spectrometry (2007) J. Anal. At. Spectrom., 22, pp. 553-560Dědina, J., Tsalev, D.L., (1995) Hydride Generation Atomic Absorption Spectrometry, , John Wiley & Sons, New YorkFigueiredo, E.C., Dědina, J., Arruda, M.A.Z., Metal furnace heated by flame as a hydride atomizer for atomic absorption spectrometry: Sb determination in environmental and pharmaceutical samples (2007) Talanta, 73, pp. 621-628Bergamin, H., Zagato, E.A.G., Krug, F.J., Reis, B.F., Merging zones in flow injection analysis Part 1. Double proportional injector and reagent consumption (1978) Anal. Chim. Acta, 101, pp. 17-23Nunes, D.L., Santos, E.P., Barin, J.S., Mortari, S.R., Dressler, V.L., Flores, E.M.M., Interference of nitrite and nitrogen dioxide on mercury and selenium determination by chemical vapor generation atomic absorption spectrometry (2005) Spectrochim. Acta Part B, 60, pp. 731-736Sabe, R., Rubio, R., García-Beltrán, L., Study of preanalytical conditions for selenium determination in urine (2001) Clin. Biochem., 34, pp. 531-555Ribeiro, A.S., Vieira, M.A., Curtius, A.J., Determination of hydride forming elements (As, Sb, Se, Sn) and Hg in environmental reference materials as acid slurries by on-line hydride generation inductively coupled plasma mass spectrometry (2004) Spectrochim. Acta Part B, 59, pp. 243-253Ribeiro, A.S., Arruda, M.A.Z., Cadore, S., Determination of bismuth in metallurgical materials using a quartz tube atomizer with tungsten coil and flow injection-hydride-generation atomic absorption spectrometry (2002) Spectrochim. Acta Part B, 57, pp. 2113-2120Gáspár, A., Berndt, H., Thermospray flame furnace atomic absorption spectrometry (TS-FF-AAS) - a simple method for trace element determination with microsamples in the mg/L concentration range (2000) Spectrochim. Acta Part B, 55, pp. 587-597Stripeikis, J., Tudino, M.B., Troccoli, O., Wuilloud, R., Olsina, R., Martinez, L., On-line copper and iron removal and selenium (VI) pre-reduction for the determination of total selenium by flow-injection hydride generation-inductively coupled plasma optical emission spectrometry (2001) Spectrochim. Acta Part B, 56, pp. 93-100Dědina, J., Atomization of volatile compounds for atomic absorption and atomic fluorescence spectrometry: on the way towards the ideal atomizer (2007) Spectrochim. Acta Part B, 62, pp. 846-872Bye, R., Iron(III) as releasing agent for copper interference in the determination of selenium by hydride-generation atomic absorption spectrometry (1987) Anal. Chim. Acta, 192, pp. 115-117Sahin, F., Volkan, M., Ataman, O.Y., Effect of nitric acid for equal stabilization and sensitivity of different selenium species in electrothermal atomic absorption spectrometry (2005) Anal. Chim. Acta, 547, pp. 126-131Niedzielski, P., The new concept of hyphenated analytical system: Simultaneous determination of inorganic arsenic (III), arsenic (V), selenium (IV) and selenium (VI) by using high performance liquid chromatography-hydride generation-(fast sequential) atomic absorption spectrometry during single analysis (2005) Anal. Chim. Acta, 551, pp. 199-206Maleki, N., Safavi, A., Doroodmand, M., Determination of selenium in water and soil by hydride generation atomic absorption spectrometry using solid reagents (2005) Talanta, 66, pp. 858-862Muñiz-Naveiro, Ó., Domínguez-González, R., Bermejo-Barrera, A., Bermejo-Barrera, P., Cocho, J.A., Fraga, J.M., Selenium speciation in cow milk obtained after supplementation with different selenium forms to the cow feed using liquid chromatography coupled with hydride generation-atomic fluorescence spectrometry (2007) Talanta, 71, pp. 1587-1593Fragueiro, S., Lavilla, I., Bendicho, C., Hydride generation-headspace single-drop microextraction-electrothermal atomic absorption spectrometry method for determination of selenium in waters after photoassisted prereduction (2006) Talanta, 68, pp. 1096-1101Arruda, M.A.Z., Gallego, M., Valcárcel, M., Determination of selenium in fruit juices by flow injection electrothermal atomization atomic absorption spectrometry (1994) J. Anal. At. Spectrom., 9, pp. 657-662Freschi, G.P.G., Freschi, C.D., Oliveira, S.R., Gomes, N.J.A., Evaluation of Ir-based modifiers in the simultaneous determination of As, Bi, Pb, Sb, and Se in milk by graphite furnace atomic absorption spectrometry (2006) At. Spectr., 27, pp. 179-185Takase, I., Luna, A.S., Campos, R.C., The use of 2-2-thiazolylazo-p-cresol to minimize the interference of Ni and Cu for the bismuth determination in alloys by hydride generation atomic absorption spectrometry (2003) Talanta, 61, pp. 597-602