Artículos de revistas
Recovery Of Dna For The Detection And Genotyping Of Human Papillomavirus From Clinical Cervical Specimens Stored For Up To 2 Years In A Universal Collection Medium With Denaturing Reagent
Registro en:
Journal Of Virological Methods. , v. 147, n. 2, p. 333 - 337, 2008.
1660934
10.1016/j.jviromet.2007.09.014
2-s2.0-37449002215
Autor
Campos E.A.
Simoes J.A.
Rabelo-Santos S.H.
Sarian L.O.
Pitta D.R.
Levi J.E.
Derchain S.
Institución
Resumen
The recovery and stability of DNA for the detection and genotyping of HPV in UCM-containing specimens, after exposure to denaturing reagents and stored for up to 2 years were evaluated. Samples were collected from 60 women who had cervical cytology specimens harboring cervical intraepithelial neoplasia (CIN) 2 or 3. All samples were stored in UCM and had been frozen at -20 °C following the addition of the denaturing reagent (sodium hydroxide) and the removal of the aliquot required for Hybrid Capture 2 testing for the identification of HPV DNA. The samples had been stored for 6, 12 and 24 months (20 samples for each storage time). HPV DNA extraction was performed according to a protocol designed specifically and the presence and quality of DNA was confirmed by human β-globin detection using the consensus primers G73 and G74. HPV DNA was amplified using the consensus primers PGMY09 and PGMY11, and reverse line-blot hybridization was used to detect type-specific amplicons for 37 HPV types. The DNA extracted from the denatured specimen was recovered in 57/60 (95%) of the samples. HPV DNA was detected in 56/57 (98%) of the recovered samples. Twenty-six of the 56 samples recovered (48%) were genotyped successfully. © 2007 Elsevier B.V. All rights reserved. 147 2 333 337 Arbyn, A., Sasieni, P., Meijer, C.J.L.M., Clavel, C., Koliopoulos, G., Dillner, J., Clinical applications of HPV testing: a summary of meta-analyses (2006) Vaccine, 24 S3, pp. 79-89 Ben-Ezra, J., Johnson, D.A., Rossi, J., Cook, N., Wu, A., Effect of fixation on the amplification of nucleic acids from paraffin-embedded material by the polymerase chain reaction (1991) J. Histochem. Cytochem., 39, pp. 351-354 Carozzi, F.M., Del Mistro, A., Confortini, M., Sani, C., Puliti, D., Trevisan, R., De Marco, L., Ronco, G., Reproducibility of HPV DNA testing by Hybrid Capture 2 in a screening setting (2005) Am. J. Clin. Pathol., 124, pp. 716-721 Carozzi, F., Bisanzi, S., Sani, C., Zappa, M., Cecchini, S., Ciatto, S., Confortini, M., Agreement between the Amplicor human papillomavirus test and the Hybrid Capture 2 assay in detection of high-risk human papillomavirus and diagnosis (2007) J. Clin. Microbiol., 45, pp. 364-369 Castellsagué, X., Diaz, M., de Sanjose, S., Munõz, N., Herrero, R., Franceschi, S., Worldwide human papillomavirus etiology of cervical adenocarcinoma and its cofactores: implications for screening and prevention (2006) J. Natl. Cancer Inst., 98, pp. 303-315 Castle, P.E., Lorincz, A.T., Scott, D.R., Sherman, M.E., Glass, A.G., Rush, B.B., Wacholder, S., Schiffman, M., Comparison between prototype Hybrid Capture 3 and hybrid capture 2 human papillomavirus DNA assay for detection of high grade cervical intraepithelial neoplasia and cancer (2003) J. Clin. Microbiol., pp. 4022-4030 Clavel, C., Masure, M., Bory, J.P., Putaud, I., Mangeonjean, C., Lorenzato, M., Gabriel, R., Birembaut, P., Hybrid Capture II-based human papillomavirus detection, a sensitive test to detect in routine high-grade cervical lesions: a preliminary study on 1518 women (1999) Br. J. Cancer, 80, pp. 1306-1311 Clifford, G., Franceschi, S., Diaz, M., Munõz, N., Villa, L.L., HPV type-distribuition in women with and without cervical neoplastic diseases (2006) Vaccine, 24 S3, pp. 26-34 Dunn, S.T., Allen, R.A., Wang, S., Walker, J., Schiffman, M., DNA extraction: an understudied and important aspect of HPV genotyping using PCR-based methods (2007) J. Virol. Methods, 143, pp. 45-54 Giuliani, L., Coletti, A., Syrjänen, K., Favalli, C., Ciotti, M., Comparison of sequencing and Roche Linear Array® in human papillomavirus (HPV) genotyping (2006) Anticancer Res., 26, pp. 3939-3941 Gravitt, P.E., Peyton, C.L., Alessi, T.Q., Wheeler, C.M., Coutlee, F., Hildesheim, A., Schiffman, M.H., Apple, R.J., Improved amplification of genital human papillomaviruses (2000) J. Clin. Microbiol., 38, pp. 357-361 Greer, C.E., Peterson, S.L., Kiviat, N.B., Manos, M.M., PCR amplification from paraffin-embedded tissues. Effects of fixative and fixation time (1991) Am. J. Clin. Pathol., 95, pp. 117-124 Levi, J.E., Kleter, B., Quint, W.G., Fink, M.C., Canto, C.L., Matsubara, R., Linhares, I., Van Doorn, L.J., High prevalence of human papillomavirus (HPV) infections and high frequency of multiple HPV genotypes in human immunodeficiency virus-infected women in Brazil (2002) J. Clin. Microbiol., 40 (9), pp. 3341-3345 Melo, A., Roa, I., Montenegro, S., Capurro, I., Roa, J.C., Detection of human papillomavirus in cytologic samples or biopsies of the cervix (2005) Rev. Med. Chil., 133, pp. 639-644 Mesquita, R.A., Anzai, E.K., Oliveira, R.N., Nunes, F.D., Evaluation of three methods of DNA extraction from paraffin-embedded material for the amplification of genomic DNA using PCR (2001) Pesqui. Odontol. Bras., 15, pp. 314-319 Muñoz, N., Bosch, F.X., de Sanjose, S., Herrero, R., Castellsague, X., Shah, K.V., Snijders, P.J., Meijer, C.J., Epidemiologic classification of human papillomavirus types associated with cervical cancer (2003) N. Engl. J. Med., 348, pp. 518-527. , International Agency for Research on Cancer Multicenter Cervical Cancer Study Group Munõz, N., Castellsagué, X., de González, A.B., Gissmann, L., HPV in the etiology of human cancer (2006) Vaccine, 24 S3, pp. 1-10 Nonogaki, S., Wakamatsu, A., Longatto Filho, A., Pereira, S.M.M., Utagawa, M.L., Alves, V.A.F., Di Loreto, C., Syrjänen, Hybrid Capture II and polymerase chain reaction for identifying HPV infections in samples collected in a new collection medium (2004) Acta Cytol., 48, pp. 514-520 Perrons, C., Kleter, B., Jelley, R., Jalal, H., Quint, W., Tedder, R., Detection and genotyping of human papillomavirus DNA by SPF10 and MY09/11 primers in cervical cells taken from women attending a colposcopy clinic (2002) J. Med. Virol., 67, pp. 246-252 Poljak, M., Marin, I.J., Seme, K., Vince, A., Hybrid capture II HPV test detects at least 15 human papillomavirus genotypes not included in its current high-risk probe cocktail (2002) J. Clin. Virol., 25 (SUPPL. 3), pp. S89-S97 Rabelo-Santos, S.H., Levi, J.E., Derchain, S.F., Sarian, L.O., Zeferino, L.C., Messias, S., Moraes, D.L., Syrjanen, K.J., DNA recovery from hybrid capture II samples stored in specimen transport medium with denaturing reagent, for the detection of human papillomavirus by PCR (2005) J. Virol. Methods, 126, pp. 197-201 Schiffman, M., Integration of papillomavirus vaccination, cytology and human papillomavirus testing (2007) Cancer Cytopathol., 111, pp. 145-153 Stevens, M.P., Rudland, E., Garland, S.M., Tabrizi, S.N., Assessment of MagNa pure LC extraction system for detection of human papillomavirus (HPV) DNA in PreservCyt samples by Roche Amplicor and Linear Array HPV tests (2006) J. Clin. Microbiol., 44, pp. 2428-2433 Stevens, M.P., Garland, S.M., Rudland, E., Tan, J., Quinn, M.A., Tabrizi, S.N., Comparison of the digene Hybrid Capture 2 assay and Roche Amplicor and Linear Array human papillomavirus (HPV) tests in detecting high-risk HPV genotypes in specimens from women with previous abnormal pap smear results (2007) J. Clin. Microbiol., 45, pp. 2130-2137 Taha, N.S., Focchi, J., Ribalta, J.C., Castelo, A., Lorincz, A., Dôres, G.B., Universal collection medium (UCM) is as suitable as the standard transport medium (STM) for Hybrid Capture II (HC-2) assay (2006) J. Clin. Virol., 36, pp. 32-35 Walboomers, J.M., Jacobs, M.V., Manos, M.M., Bosch, F.X., Kummer, J.A., Shah, K.V., Snijders, P.J., Munoz, N., Human papillomavirus is a necessary cause of invasive cervical cancer worldwide (1999) J. Pathol., 189, pp. 12-19 Woo, Y.L., Damay, I., Stanley, M., Crawford, R., Sterling, J., The use of HPV Linear Array for multiple HPV typing on archival frozen tissue and DNA specimens (2007) J. Virol. Methods, 142, pp. 226-230