dc.creator | Peroni L.A. | |
dc.creator | Reis J.R.R.d. | |
dc.creator | Coletta-Filho H.D. | |
dc.creator | de Souza A.A. | |
dc.creator | Machado M.A. | |
dc.creator | Stach-Machado D.R. | |
dc.date | 2008 | |
dc.date | 2015-06-30T19:29:23Z | |
dc.date | 2015-11-26T14:44:42Z | |
dc.date | 2015-06-30T19:29:23Z | |
dc.date | 2015-11-26T14:44:42Z | |
dc.date.accessioned | 2018-03-28T21:53:39Z | |
dc.date.available | 2018-03-28T21:53:39Z | |
dc.identifier | | |
dc.identifier | Journal Of Microbiological Methods. , v. 75, n. 2, p. 302 - 307, 2008. | |
dc.identifier | 1677012 | |
dc.identifier | 10.1016/j.mimet.2008.06.024 | |
dc.identifier | http://www.scopus.com/inward/record.url?eid=2-s2.0-50549086775&partnerID=40&md5=60135ad7f5b1c19150cc3d7b96637e89 | |
dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/106446 | |
dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/106446 | |
dc.identifier | 2-s2.0-50549086775 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1252231 | |
dc.description | Xylella fastidiosa causes significant losses in many economically important crops. An efficient pathogen detection system is critical for epidemiology studies, particularly when large sample size is involved. In this study we report the development of immunomolecular assays like Immmunocapture-PCR and Immuno-PCR for direct detection of X. fastidiosa without DNA isolation. Whereas the reactivity of ELISA and PCR ranged from 106 to 104 bacterial cells, the IC-PCR sensitivity was up to 103 and the detection limit of I-PCR was up to 101 bacterial cells. These methods can use either plant sample extracts or cultivated media, and show no cross reaction for any other endophytic citrus-bacteria. Therefore, IC-PCR and I-PCR assays provide an alternative for quick and very sensitive methods to screening X. fastidiosa, with the advantage of not requiring any concentration or DNA purification steps while still allowing an accurate diagnosis of CVC. © 2008 Elsevier B.V. All rights reserved. | |
dc.description | 75 | |
dc.description | 2 | |
dc.description | 302 | |
dc.description | 307 | |
dc.description | Adler, M., Immuno-PCR as a clinical laboratory tool (2005) Adv. Clin. Chem., 39, pp. 239-292 | |
dc.description | Allen, R.C., Rogelj, S., Cordova, S.E., Kieft, T.L., An immune-PCR method for detecting Bacillus thuringiensis Cry1Ac toxin (2006) J. Immunol. Methods, 308, pp. 109-115 | |
dc.description | Almeida, R.P.P., Pereira, E.F., Purcell, A.H., Lopes, J.R.S., Multiplication and movement of a citrus strain of Xylella fastidiosa within sweet orange (2001) Plant Disease, 85, pp. 382-386 | |
dc.description | Barletta, J., Applications of real-time immuno-polymerase chain reaction (rt-IPCR) for the rapid diagnoses of viral antigens and pathologic proteins (2006) Mol. Aspects Med., 27, pp. 224-252 | |
dc.description | Beretta, M.J.G., Harakava, R., Chagas, C.M., First report of Xylella fastidiosa in coffee (1996) Plant Dis., 80, p. 821 | |
dc.description | Chang, C.J., Garnier, M., Zreik, L., Rosseti, V., Bové, M., Citrus variegated chlorosis: cultivation of the causal bacterium and experimental reproduction of the disease (1993) Proc. 12th Conf. IOCV, pp. 294-305. , Moreno P., Da Graça J.V., and Timmer L.W. (Eds) | |
dc.description | Chao, H.Y., Wang, Y.C., Tang, S.S., Liu, H.W., A highly sensitive immuno-polymerase chain reaction assay for Clostridium botulinum neurotoxin type A (2004) Toxicon, 43, pp. 27-34 | |
dc.description | Chen, J., Grove, E.L., Civerolo, M., Viveres, M., Freeman, M., Zheng, Y., Two Xylella fastidiosa genotypes associated with almond leaf scorch disease on the same location in California (2005) Phytopathology, 95, pp. 708-714 | |
dc.description | Chye, S.M., Lin, S.R., Chen, Y.L., Chung, L.Y., Yen, C.M., Immuno-PCR for detection of antigen to Angiostrongylus cantonensis circulating fifth-stage worms (2004) Clin. Chem., 50, pp. 51-57 | |
dc.description | Ciapina, L.P., Carareto Alves, L.M., Lemos, E.G.M., A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers (2004) J. Appl. Microbiol., 96, pp. 546-551 | |
dc.description | Clark, M.F., Lister, R.M., Bar-Joseph, M., ELISA techniques (1986) Methods Enzymol., 118, pp. 742-766 | |
dc.description | Coletta-Filho, H.D., Machado, M.A., Targon, M.L.P.N., Detecção de Xylella fastidiosa da clorose variegada dos citros através de PCR (1996) Fitopatol. Bras. Supl., p. 343 | |
dc.description | Davis, M.J., Purcell, A.H., Thomson, S.V., Isolation media for the Pierce's disease bacterium (1981) Phytopathology, 73, pp. 1510-1515 | |
dc.description | Fischer, A., von Eiff, C., Kuczius, T., Omoe, K., Peters, G., Becker, K., A quantitative real-time immuno-PCR approach for detection of staphylococcal enterotoxins (2007) J. Mol. Med., 85, pp. 461-469 | |
dc.description | FUNDECITRUS, Fundo de Defesa da Citricultura, 2007. http://www.fundecitrus.com.brHeggeness, M.H., Ash, J.F., Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy (1977) J. Cell Biol., 73, pp. 783-788 | |
dc.description | Henson, J., French, R., The polymerase chain reaction and plant disease diagnosis (1993) Annu. Rev. Phytopathol., 31, pp. 81-109 | |
dc.description | Hopkins, D.L., Xylella fastidiosa: xylem-limited bacterial pathogen of plants (1989) Annu. Rev. Phytopathol., 27, pp. 271-290 | |
dc.description | Kamatsu, M., Kobayashi, D., Saito, K., Furuya, D., Yagihashi, A., Araake, H., Tsuji, N., Watanabe, N., Tumor necrosis factor-a in serum of patient with inflammatory bowel disease as measured by a highly sensitive immuno-PCR (2001) Clin. Chem., 47, pp. 1297-1301 | |
dc.description | Liang, H., Cordova, S.E., Kieft, T.L., Rogelj, S., A highly sensitive immuno-PCR assay for detecting Group A Streptococcus (2003) J. Immunol. Methods, 279, pp. 101-110 | |
dc.description | Li, W.-B., Pria Jr., W.D., Lacava, P.M., Qin, X., Hartung, J.S., Presence of Xylella fastidiosa in sweet orange fruit and seeds and its transmission to seedlings (2003) Phytopathology, 93, pp. 953-958 | |
dc.description | Lubelli, C., Chatgilialoglu, A., Bolognesi, A., Strocchi, P., Colombatti, M., Stirpe, F., Detection of ricin and other ribosome-inactivating proteins by an immuno-polymerase chain reaction assay (2006) Anal. Biochem., 355, pp. 102-109 | |
dc.description | Luo, W., Wang, S., Peng, X., Identification of Shiga toxin-producing bacteria by a new immune-capture toxin gene PCR (2002) FEMS Microbiol. Lett., 216, pp. 39-42 | |
dc.description | Mason, J.T., Xu, L., Sheng, Z.M., He, J., O'Leary, T.J., Liposome polymerase chain reaction assay for the sub-attomolar detection of cholera toxin and botulinum neurotoxin type A (2006) Nat. Protocols, 1, pp. 2003-2011 | |
dc.description | Minsavage, G.V., Thompson, C.M., Hopkins, D.L., Leite, R.M.V.B.C., Stall, R.E., Development of a polymerase chain reaction protocol for detection of Xylella fastidiosa in plant tissue (1994) Phytopathology, 84, pp. 456-461 | |
dc.description | Niemeyer, C.M., Adler, M., Wacker, R., Immuno-PCR: high sensitivity detection of proteins by nucleic acid amplification (2005) Trends Biotechnol., 23, pp. 209-216 | |
dc.description | Pooler, M.R., Hartung, J.S., Specific PCR detection and identification of Xylella fastidiosa strains causing citrus variegated chlorosis (1995) Curr. Microbiol., 31, pp. 377-381 | |
dc.description | Purcell, A.H., Hopkins, D.L., Fastidious xylem-limited bacterial plant pathogens (1996) Annu. Rev. Phytopathol., 4, pp. 131-151 | |
dc.description | Rosseti, V., Garnier, M., Breve, J.M., Beretta, M.J.G., Teixeira, A.R.R., Quaggio, J.A., De Negri, J.D., Présence de bactéries dans le xylème d' orangers atteints de chlorose variégeé une novelle maladié des agrumes du Brésil (1990) C. R. Acad. Sci. Paris, 310, pp. 345-349 | |
dc.description | Sanna, P.P., Weiss, F., Samson, M.E., Bloom, F.E., Pich, E.M., Rapid induction of tumor necrosis factor a in the cerebrospinal fluid after intracerebroventricular injection of lipopolysaccharide revealed by a sensitive capture immuno-PCR assay (1995) Proc. Natl. Acad. Sci. USA, 92, pp. 272-275 | |
dc.description | Sano, T., Smith, C.L., Cantor, C.R., Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates (1992) Science, 258, pp. 120-122 | |
dc.description | Schaad, N.W., Postnikova, E., Lacy, G., Fatmi, M., Chang, C., Xylella fastidiosa subspecies: X. fastidiosa subsp. piercei, subsp. nov., X. fastidiosa subsp. multiplex subsp. nov., and X. fastidiosa subsp. pauca subsp. nov (2004) Syst. Appl. Microbiol., 27, pp. 290-300 | |
dc.description | Schneider, B.J., Zhao, J.L., Orser, C.S., Detection of Clavibacter michiganensis subsp. sepedonicus by DNA amplification (1993) FEMS Microbiol. Lett., 109, pp. 207-212 | |
dc.description | Sims, P.W., Vasser, M., Wong, W.L., Williams, P.M., Me, Y.G., Immunopolymerase chain reaction using real-time polymerase chain reaction for detection (2000) Anal. Biochem., 281, pp. 230-232 | |
dc.description | Souza, A.A., Takita, M.A., Coletta-Filho, H.D., Caldana, C., Goldman, G.H., Yanai, G.M., Muto, N.H., Machado, M.A., Analysis of gene expression in two growth states of Xylella fastidiosa and its relationship with pathogenicity (2003) Mol. Plant Microbe Interact., 16, pp. 867-875 | |
dc.description | Wilson, I.G., Inhibition and facilitation of nucleic acid amplification (1997) Appl. Environ. Microbiol., 63, pp. 3741-3751 | |
dc.description | Zhou, H., Fisher, R.J., Papas, T.S., Universal immuno-PCR for ultra-sensitive target protein detection (1993) Nucleic Acids Res., 21, pp. 6038-6039 | |
dc.language | en | |
dc.publisher | | |
dc.relation | Journal of Microbiological Methods | |
dc.rights | fechado | |
dc.source | Scopus | |
dc.title | Assessment Of The Diagnostic Potential Of Immmunocapture-pcr And Immuno-pcr For Citrus Variegated Chlorosis | |
dc.type | Artículos de revistas | |