dc.creatorPeroni L.A.
dc.creatorReis J.R.R.d.
dc.creatorColetta-Filho H.D.
dc.creatorde Souza A.A.
dc.creatorMachado M.A.
dc.creatorStach-Machado D.R.
dc.date2008
dc.date2015-06-30T19:29:23Z
dc.date2015-11-26T14:44:42Z
dc.date2015-06-30T19:29:23Z
dc.date2015-11-26T14:44:42Z
dc.date.accessioned2018-03-28T21:53:39Z
dc.date.available2018-03-28T21:53:39Z
dc.identifier
dc.identifierJournal Of Microbiological Methods. , v. 75, n. 2, p. 302 - 307, 2008.
dc.identifier1677012
dc.identifier10.1016/j.mimet.2008.06.024
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-50549086775&partnerID=40&md5=60135ad7f5b1c19150cc3d7b96637e89
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/106446
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/106446
dc.identifier2-s2.0-50549086775
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1252231
dc.descriptionXylella fastidiosa causes significant losses in many economically important crops. An efficient pathogen detection system is critical for epidemiology studies, particularly when large sample size is involved. In this study we report the development of immunomolecular assays like Immmunocapture-PCR and Immuno-PCR for direct detection of X. fastidiosa without DNA isolation. Whereas the reactivity of ELISA and PCR ranged from 106 to 104 bacterial cells, the IC-PCR sensitivity was up to 103 and the detection limit of I-PCR was up to 101 bacterial cells. These methods can use either plant sample extracts or cultivated media, and show no cross reaction for any other endophytic citrus-bacteria. Therefore, IC-PCR and I-PCR assays provide an alternative for quick and very sensitive methods to screening X. fastidiosa, with the advantage of not requiring any concentration or DNA purification steps while still allowing an accurate diagnosis of CVC. © 2008 Elsevier B.V. All rights reserved.
dc.description75
dc.description2
dc.description302
dc.description307
dc.descriptionAdler, M., Immuno-PCR as a clinical laboratory tool (2005) Adv. Clin. Chem., 39, pp. 239-292
dc.descriptionAllen, R.C., Rogelj, S., Cordova, S.E., Kieft, T.L., An immune-PCR method for detecting Bacillus thuringiensis Cry1Ac toxin (2006) J. Immunol. Methods, 308, pp. 109-115
dc.descriptionAlmeida, R.P.P., Pereira, E.F., Purcell, A.H., Lopes, J.R.S., Multiplication and movement of a citrus strain of Xylella fastidiosa within sweet orange (2001) Plant Disease, 85, pp. 382-386
dc.descriptionBarletta, J., Applications of real-time immuno-polymerase chain reaction (rt-IPCR) for the rapid diagnoses of viral antigens and pathologic proteins (2006) Mol. Aspects Med., 27, pp. 224-252
dc.descriptionBeretta, M.J.G., Harakava, R., Chagas, C.M., First report of Xylella fastidiosa in coffee (1996) Plant Dis., 80, p. 821
dc.descriptionChang, C.J., Garnier, M., Zreik, L., Rosseti, V., Bové, M., Citrus variegated chlorosis: cultivation of the causal bacterium and experimental reproduction of the disease (1993) Proc. 12th Conf. IOCV, pp. 294-305. , Moreno P., Da Graça J.V., and Timmer L.W. (Eds)
dc.descriptionChao, H.Y., Wang, Y.C., Tang, S.S., Liu, H.W., A highly sensitive immuno-polymerase chain reaction assay for Clostridium botulinum neurotoxin type A (2004) Toxicon, 43, pp. 27-34
dc.descriptionChen, J., Grove, E.L., Civerolo, M., Viveres, M., Freeman, M., Zheng, Y., Two Xylella fastidiosa genotypes associated with almond leaf scorch disease on the same location in California (2005) Phytopathology, 95, pp. 708-714
dc.descriptionChye, S.M., Lin, S.R., Chen, Y.L., Chung, L.Y., Yen, C.M., Immuno-PCR for detection of antigen to Angiostrongylus cantonensis circulating fifth-stage worms (2004) Clin. Chem., 50, pp. 51-57
dc.descriptionCiapina, L.P., Carareto Alves, L.M., Lemos, E.G.M., A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers (2004) J. Appl. Microbiol., 96, pp. 546-551
dc.descriptionClark, M.F., Lister, R.M., Bar-Joseph, M., ELISA techniques (1986) Methods Enzymol., 118, pp. 742-766
dc.descriptionColetta-Filho, H.D., Machado, M.A., Targon, M.L.P.N., Detecção de Xylella fastidiosa da clorose variegada dos citros através de PCR (1996) Fitopatol. Bras. Supl., p. 343
dc.descriptionDavis, M.J., Purcell, A.H., Thomson, S.V., Isolation media for the Pierce's disease bacterium (1981) Phytopathology, 73, pp. 1510-1515
dc.descriptionFischer, A., von Eiff, C., Kuczius, T., Omoe, K., Peters, G., Becker, K., A quantitative real-time immuno-PCR approach for detection of staphylococcal enterotoxins (2007) J. Mol. Med., 85, pp. 461-469
dc.descriptionFUNDECITRUS, Fundo de Defesa da Citricultura, 2007. http://www.fundecitrus.com.brHeggeness, M.H., Ash, J.F., Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy (1977) J. Cell Biol., 73, pp. 783-788
dc.descriptionHenson, J., French, R., The polymerase chain reaction and plant disease diagnosis (1993) Annu. Rev. Phytopathol., 31, pp. 81-109
dc.descriptionHopkins, D.L., Xylella fastidiosa: xylem-limited bacterial pathogen of plants (1989) Annu. Rev. Phytopathol., 27, pp. 271-290
dc.descriptionKamatsu, M., Kobayashi, D., Saito, K., Furuya, D., Yagihashi, A., Araake, H., Tsuji, N., Watanabe, N., Tumor necrosis factor-a in serum of patient with inflammatory bowel disease as measured by a highly sensitive immuno-PCR (2001) Clin. Chem., 47, pp. 1297-1301
dc.descriptionLiang, H., Cordova, S.E., Kieft, T.L., Rogelj, S., A highly sensitive immuno-PCR assay for detecting Group A Streptococcus (2003) J. Immunol. Methods, 279, pp. 101-110
dc.descriptionLi, W.-B., Pria Jr., W.D., Lacava, P.M., Qin, X., Hartung, J.S., Presence of Xylella fastidiosa in sweet orange fruit and seeds and its transmission to seedlings (2003) Phytopathology, 93, pp. 953-958
dc.descriptionLubelli, C., Chatgilialoglu, A., Bolognesi, A., Strocchi, P., Colombatti, M., Stirpe, F., Detection of ricin and other ribosome-inactivating proteins by an immuno-polymerase chain reaction assay (2006) Anal. Biochem., 355, pp. 102-109
dc.descriptionLuo, W., Wang, S., Peng, X., Identification of Shiga toxin-producing bacteria by a new immune-capture toxin gene PCR (2002) FEMS Microbiol. Lett., 216, pp. 39-42
dc.descriptionMason, J.T., Xu, L., Sheng, Z.M., He, J., O'Leary, T.J., Liposome polymerase chain reaction assay for the sub-attomolar detection of cholera toxin and botulinum neurotoxin type A (2006) Nat. Protocols, 1, pp. 2003-2011
dc.descriptionMinsavage, G.V., Thompson, C.M., Hopkins, D.L., Leite, R.M.V.B.C., Stall, R.E., Development of a polymerase chain reaction protocol for detection of Xylella fastidiosa in plant tissue (1994) Phytopathology, 84, pp. 456-461
dc.descriptionNiemeyer, C.M., Adler, M., Wacker, R., Immuno-PCR: high sensitivity detection of proteins by nucleic acid amplification (2005) Trends Biotechnol., 23, pp. 209-216
dc.descriptionPooler, M.R., Hartung, J.S., Specific PCR detection and identification of Xylella fastidiosa strains causing citrus variegated chlorosis (1995) Curr. Microbiol., 31, pp. 377-381
dc.descriptionPurcell, A.H., Hopkins, D.L., Fastidious xylem-limited bacterial plant pathogens (1996) Annu. Rev. Phytopathol., 4, pp. 131-151
dc.descriptionRosseti, V., Garnier, M., Breve, J.M., Beretta, M.J.G., Teixeira, A.R.R., Quaggio, J.A., De Negri, J.D., Présence de bactéries dans le xylème d' orangers atteints de chlorose variégeé une novelle maladié des agrumes du Brésil (1990) C. R. Acad. Sci. Paris, 310, pp. 345-349
dc.descriptionSanna, P.P., Weiss, F., Samson, M.E., Bloom, F.E., Pich, E.M., Rapid induction of tumor necrosis factor a in the cerebrospinal fluid after intracerebroventricular injection of lipopolysaccharide revealed by a sensitive capture immuno-PCR assay (1995) Proc. Natl. Acad. Sci. USA, 92, pp. 272-275
dc.descriptionSano, T., Smith, C.L., Cantor, C.R., Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates (1992) Science, 258, pp. 120-122
dc.descriptionSchaad, N.W., Postnikova, E., Lacy, G., Fatmi, M., Chang, C., Xylella fastidiosa subspecies: X. fastidiosa subsp. piercei, subsp. nov., X. fastidiosa subsp. multiplex subsp. nov., and X. fastidiosa subsp. pauca subsp. nov (2004) Syst. Appl. Microbiol., 27, pp. 290-300
dc.descriptionSchneider, B.J., Zhao, J.L., Orser, C.S., Detection of Clavibacter michiganensis subsp. sepedonicus by DNA amplification (1993) FEMS Microbiol. Lett., 109, pp. 207-212
dc.descriptionSims, P.W., Vasser, M., Wong, W.L., Williams, P.M., Me, Y.G., Immunopolymerase chain reaction using real-time polymerase chain reaction for detection (2000) Anal. Biochem., 281, pp. 230-232
dc.descriptionSouza, A.A., Takita, M.A., Coletta-Filho, H.D., Caldana, C., Goldman, G.H., Yanai, G.M., Muto, N.H., Machado, M.A., Analysis of gene expression in two growth states of Xylella fastidiosa and its relationship with pathogenicity (2003) Mol. Plant Microbe Interact., 16, pp. 867-875
dc.descriptionWilson, I.G., Inhibition and facilitation of nucleic acid amplification (1997) Appl. Environ. Microbiol., 63, pp. 3741-3751
dc.descriptionZhou, H., Fisher, R.J., Papas, T.S., Universal immuno-PCR for ultra-sensitive target protein detection (1993) Nucleic Acids Res., 21, pp. 6038-6039
dc.languageen
dc.publisher
dc.relationJournal of Microbiological Methods
dc.rightsfechado
dc.sourceScopus
dc.titleAssessment Of The Diagnostic Potential Of Immmunocapture-pcr And Immuno-pcr For Citrus Variegated Chlorosis
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución