Study Of Optically Trapped Living Trypanosoma Cruzi/trypanosoma Rangeli - Rhodnius Prolixus Interactions By Real Time Confocal Images Using Cdse Quantum Dots

dc.creatorDe Thomaz A.A.
dc.creatorAlmeida D.B.
dc.creatorFaustino W.M.
dc.creatorJacob G.J.
dc.creatorFontes A.
dc.creatorBarbosa L.C.
dc.creatorCesar C.L.
dc.creatorStahl C.V.
dc.creatorSantos-Mallet J.R.
dc.creatorGomes S.A.O.
dc.creatorFeder D.
dc.date2008
dc.date2015-06-30T19:18:47Z
dc.date2015-11-26T14:41:55Z
dc.date2015-06-30T19:18:47Z
dc.date2015-11-26T14:41:55Z
dc.date.accessioned2018-03-28T21:49:11Z
dc.date.available2018-03-28T21:49:11Z
dc.identifier9780819472588
dc.identifierProceedings Of Spie - The International Society For Optical Engineering. , v. 7038, n. , p. - , 2008.
dc.identifier0277786X
dc.identifier10.1117/12.795370
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-56249118732&partnerID=40&md5=62bfa0be6bd4ee0fff8cedc9c81514e0
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/105706
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/105706
dc.identifier2-s2.0-56249118732
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1251054
dc.descriptionOne of the fundamental goals in biology is to understand the interplay between biomolecules of different cells. This happen, for example, in the first moments of the infection of a vector by a parasite that results in the adherence to the cell walls. To observe this kind of event we used an integrated Optical Tweezers and Confocal Microscopy tool. This tool allow us to use the Optical Tweezers to trigger the adhesion of the Trypanosoma cruzi and Trypanosoma rangeli parasite to the intestine wall cells and salivary gland of the Rhodnius prolixus vector and to, subsequently observe the sequence of events by confocal fluorescence microscopy under optical forces stresses. We kept the microorganism and vector cells alive using CdSe quantum dot staining. Besides the fact that Quantum Dots are bright vital fluorescent markers, the absence of photobleaching allow us to follow the events in time for an extended period. By zooming to the region of interested we have been able to acquire confocal images at the 2 to 3 frames per second rate.
dc.description7038
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dc.languageen
dc.publisher
dc.relationProceedings of SPIE - The International Society for Optical Engineering
dc.rightsaberto
dc.sourceScopus
dc.titleStudy Of Optically Trapped Living Trypanosoma Cruzi/trypanosoma Rangeli - Rhodnius Prolixus Interactions By Real Time Confocal Images Using Cdse Quantum Dots
dc.typeActas de congresos


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