Hydrophobic liquid membranes have a high technological potential in many fields of separation science. The dynamics of these systems is very complex and still not fully understood. In this work we studied the effect of the incorporation of cationic and anionic l-tryptophan at pH 1.8 and 10.0, respectively, in Aerosol-OT reverse micelles performing small angle X-ray scattering experiments. The use of a synchrotron radiation source allowed efficient in situ data acquisition. Several insights on l-tryptophan transport dynamics through hydrophobic membranes containing AOT could be obtained from these SAXS experiments, such as amino acid site localization and changes in the reverse micelle sizes. © 2007 Elsevier Inc. All rights reserved.31815967Tondre, C., Hebrant, M., (1997) J. Mol. Liq., 72, p. 279Nghiem, L.D., Mornane, P., Potter, I.D., Perera, J.M., Cattrall, R.W., Kolev, S.D., (2006) J. Membr. Sci., 281, p. 7Juang, R.S., Kao, H.C., Shiau, C.L., (2006) J. Membr. Sci., 281, p. 636Guzun, A.S., Juncu, G., Floarea, O., (1999) Chem. Eng. Technol., 22, p. 65Hebrant, M., Tondre, C., (1998) Anal. Sci., 14, p. 109Gjelstad, A., Rasmussen, K.E., Pedersen-Bjergaard, S., (2006) J. Chromatogr. A, 1124, p. 29Mulugeta, M., Megersa, N., (2004) Talanta, 64, p. 101Lamb, J.D., Christensen, J.J., Oscarson, J.L., Nielsen, B., Asay, B.W., Izatt, R.M., (1980) J. Am. Chem. Soc., 102, p. 6820Antunes, A.M., Ferreira, M.M.C., Melgo, M.S., Volpe, P.L.O., (1999) J. Mol. Struct., 481, p. 563Antunes, A.M., Ferreira, M.M.C., Volpe, P.L.O., (2002) J. Chemom., 16, p. 111Lang, J., Jada, A., Malliaris, A., (1988) J. Phys. Chem., 92, p. 1946Silber, J.J., Biasutti, A., Abuin, E., Lissi, E., (1999) Adv. Colloid Interface Sci., 82, p. 189Nave, S., Eastoe, J., Penfold, J., (2000) Langmuir, 16, p. 8733Nave, S., Eastoe, J., Heenan, R.K., Steytler, D., Grillo, I., (2000) Langmuir, 16, p. 8741Nave, S., Eastoe, J., Heenan, R.K., Steytler, D., Grillo, I., (2002) Langmuir, 18, p. 1505Nave, S., Paul, A., Eastoe, J., Pitt, A.R., Heenan, R.K., (2005) Langmuir, 21, p. 10021Correa, N.M., Levinger, N.E., (2006) J. Phys. Chem. B, 110, p. 13050Reta, M., Waymas, O., Silber, J.J., (2006) J. Phys. Org. Chem., 19, p. 219Paul, B.K., Mitra, R.K., (2006) J. Colloid Interface Sci., 295, p. 230Correa, N.M., Pires, P.A.R., Silber, J.J., El Seoud, O.A., (2005) J. Phys. Chem. B, 109, p. 21209Moulik, S.P., Paul, B.K., (1998) Adv. Colloid Interface Sci., 78, p. 99Paul, B.K., Moulik, S.P., (1997) J. Dispersion Sci. Technol., 18, p. 301Svergun, D.I., Konarev, P.V., Volkov, V.V., Koch, M.H.J., Sager, W.F.C., Smeets, J., Blokhuis, E.M., (2000) J. Chem. Phys., 113, p. 1651Robinson, B.H., Toprakcioglu, C., Dore, J.C., Chieux, P., (1984) J. Chem. Soc. Faraday Trans., 80, p. 13Kiselev, M.A., Zemlyanaya, E.V., Aswal, V.K., Neubert, R.H.H., (2006) Eur. Biophys. J. Biophys. Lett., 35, p. 477Midis, E.B., Hatton, T.A., (1990) J. Phys. Chem., 94, p. 6400Midis, E.B., Hatton, T.A., (1990) J. Phys. Chem., 94, p. 6411Midis, E.B., Bommarius, A.S., Hatton, T.A., (1991) J. Phys. Chem., 95, p. 5943Midis, E.B., Hatton, T.A., (1991) J. Phys. Chem., 95, p. 5957Leodidis, E.B., Hatton, T.A., (1991) J. Colloid Interface Sci., 147, p. 163Adachi, M., Harada, M., Shioi, A., Sato, Y., (1991) J. Phys. Chem., 95, p. 7925Sager, W.F.C., (1998) Langmuir, 14, p. 6385Svergun, D.I., (1991) J. Appl. Cryst., 24, p. 485Svergun, D.I., (1992) J. Appl. Cryst., 25, p. 495Kotlarckyk, M., Chen, S.H., (1983) J. Chem. Phys., 79, p. 2461Itri, R., Caetano, W., Barbosa, L.R.S., Baptista, M.S., (2004) Braz. J. Phys., 34, p. 58Fu, X., Li, J.L., Ma, Y., Zhang, L., Wang, D.B., Hu, Z.S., (2001) Colloids Surf. A Physicochem. Eng. Aspects, 179, p. 1Abel, S., Sterpone, F., Bandyopadhyay, S., Marchi, M., (2004) J. Phys. Chem. B, 108, p. 19458Hamada, K., Ikeda, T., Kawai, T., Kon-No, K., (2001) J. Colloid Interface Sci., 233, p. 166Chen, J., Zhang, J., Han, B., Feng, X., Hou, M., Li, W., Zhang, Z., (2006) Chem. Eur. J., 12, p. 8067Zhong-Can, O.-Y., Helfrich, W., (1989) Phys. Rev. A, 39, p. 5280Blokhuis, E.M., Sager, W.F.C., (2001) J. Chem. Phys., 115, p. 1073Fletcher, P.D.I., Howe, A.M., Robinson, B.H., (1987) J. Chem. Soc. Faraday Trans. I, 83, p. 985Evans, D.F., Wennerström, H., (1999) The Colloidal Domain: Where Physics, Chemistry, Biology and Technology Meet, , Wiley-VCH, Weinheim p. 154