Regulation Of Intracellular Calcium Homeostasis In Trypanosoma Cruzi. Effects Of Calmidazolium And Trifluoperazine

dc.creatorVercesi A.E.
dc.creatorHoffmann M.E.
dc.creatorBernardes C.F.
dc.creatorDocampo R.
dc.date1991
dc.date2015-06-30T14:09:04Z
dc.date2015-11-26T14:41:15Z
dc.date2015-06-30T14:09:04Z
dc.date2015-11-26T14:41:15Z
dc.date.accessioned2018-03-28T21:48:04Z
dc.date.available2018-03-28T21:48:04Z
dc.identifier
dc.identifierCell Calcium. , v. 12, n. 5, p. 361 - 369, 1991.
dc.identifier1434160
dc.identifier10.1016/0143-4160(91)90052-G
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0025739736&partnerID=40&md5=361cac23eacfeb14b0a0066ad3702743
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/99151
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/99151
dc.identifier2-s2.0-0025739736
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1250763
dc.descriptionTrypanosoma cruzi epimastigotes maintained an intracellular free calcium concentration of about 0.15 μM, as measured with the fluorescent indicator Fura-2. The maintenance of low [Ca2+]i is energy-dependent since it is disrupted by KCN and FCCP. When the cells were permeabilized with digitonin, the steady-state free Ca2+ concentration in the absence of ATP was about 0.7 μM. The additional presence of ATP resulted in a steady-state level close to 0.1-0.2 μM which compares favorably with the concentration detected in intact cells. Intracellular Ca2+ uptake at high levels of free Ca2+ (1 μM) was due to energy-dependent mitochondrial uptake as indicated by its FCCP-sensitivity. However, as the free Ca2+ concentration was lowered from 1 μM, essentially all uptake was due to the ATP-dependent Ca2+ sequestration by the endoplasmic reticulum as indicated by its stimulation by ATP, and its inhibition by sodium vanadate. High concentrations of the calrnodulin antagonist trifluoperazine, inhibited both the Ca2+ uptake by the endoplasmic retlculum and by the mitochondria, while calmidazolium released Ca2+ from both compartments. In addition, trifluoperazine and calmidazolium inhibited respiration and collapsed the mitochondrial membrane potential of T. cruzi, thus indicating non-specific effects unrelated to calmodulin. © 1991.
dc.description12
dc.description5
dc.description361
dc.description369
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dc.languageen
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dc.relationCell Calcium
dc.rightsfechado
dc.sourceScopus
dc.titleRegulation Of Intracellular Calcium Homeostasis In Trypanosoma Cruzi. Effects Of Calmidazolium And Trifluoperazine
dc.typeArtículos de revistas


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