Isolation And Characterization Of Hemorrhagic, Myonecrotic And Edema-inducing Toxins From Bothrops Insularis (jararaca Ilhoa) Snake Venom

dc.creatorSelistre H.S.
dc.creatorQueiroz L.S.
dc.creatorCunha O.A.B.
dc.creatorDe Souza G.E.P.
dc.creatorGiglio J.R.
dc.date1990
dc.date2015-06-30T14:02:25Z
dc.date2015-11-26T14:40:46Z
dc.date2015-06-30T14:02:25Z
dc.date2015-11-26T14:40:46Z
dc.date.accessioned2018-03-28T21:47:13Z
dc.date.available2018-03-28T21:47:13Z
dc.identifier
dc.identifierToxicon. , v. 28, n. 3, p. 261 - 273, 1990.
dc.identifier410101
dc.identifier10.1016/0041-0101(90)90062-C
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0025231599&partnerID=40&md5=51443d3d46f4f86a2a49439558ee9162
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/99097
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/99097
dc.identifier2-s2.0-0025231599
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1250545
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionBothrops insularis snake venom was fractionated by gel filtration on Sephadex G-150 followed by ion-exchange chromatography on SP-Sephadex C-25. Two active fractions were purified to homogeneity: (1) SIII-SpI, approximate mol. wt 32,000 and N-terminal amino acid residue Val. This fraction showed esterase activity on TAME, edema-inducing activity on the rat hind paw and contractile activity on the isolated guinea pig ileum. The latter two activities were antagonized by benadryl plus methysergide; (2) SIII-SpVI, a myonecrotic and edema-inducing phospholipase, approximate mol. wt 29,000, N-terminal amino acid residue pyro-Glu, consisting of two chains of approximately 15,000 mol. wt each linked by disulphide bridge(s). The induction of edema by this fraction was not antagonized by benadryl plus methysergide, indomethacin, BW755C or BN52021, but it was antagonized by dexamethasone. Three highly purified hemorrhagic heterodimeric fractions, SIII-SpIII-3, SIII-SpIII-4 and SIII-SpIII-5, of approximate mol. wts 26,000, 29,000 and 26,000, and having N-terminal residues of Asx, Asx and Gly, respectively, were further isolated by preparative polyacrylamide slab gel electrophoresis. SIII-SpIII-4 and SIII-SpIII-5 increased the recalcification time of citrated rat plasma. None of the five isolated fractions showed any proteolytic (on casein) or kininogenase activity. © 1990.
dc.description28
dc.description3
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dc.description273
dc.description2007/59779-6; CAPES; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; 2010/51307-0; FAPESP; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionAssakura, Reichl, Mandelbaum, Comparison of immunological, biochemical and biophysical properties of the three hemorrhagic factors isolated from the venom of Bothrops jararaca (jararaca) (1986) Toxicon, 24, pp. 943-946
dc.descriptionBerkson, A statistically precise and relatively simple method of estimating the bio-assay with quantal response, based on the logistic function (1953) J. Am. Stat. Ass., 48, pp. 565-599
dc.descriptionBjarnason, Tu, Hemorrhagic toxins from western diamondback rattlesnake (Crotalus atrox) venom: isolation and characterization of five toxins and role of zinc in hemorrhagic toxin e (1978) Biochemistry, 17, pp. 3395-3404
dc.descriptionBraquet, The ginkgolides (1988) From Chinese pharmacopeia to a new class of pharmacological agents: the antagonists of platelet-activating factor, 1, pp. XV-XXVI. , P. Braquet, Ginkgolides, J. R. Prous Science, Barcelona
dc.descriptionChapman, The symptomatology, pathology and treatment of the bites of venomous snakes of central and southern Africa (1968) Venomous Animals and Their Venoms, 1, pp. 463-527. , W. Bücherl, E.E. Buckley, V. Deulofeu, Academic Press, New York
dc.descriptionChiu, Chen, Teng, Edema formation and degranulation of mast cells by a basic phospholipase A2 purified from Trimeresurus mucrosquamatus snake venom (1989) Toxicon, 27, pp. 115-125
dc.descriptionDavis, Disc electrophoresis. II. Method and application to human serum proteins (1964) Ann. N.Y. Acad. Sci., 121, pp. 404-427
dc.descriptionDe Haas, Postema, Nieuwenhuizen, Van Deenen, Purification and properties of phospholipase A from porcine pancreas (1968) Biochim. biophys. Acta, 159, pp. 103-117
dc.descriptionDoolittle, Terminal pyrrolidonecarboxylic acid: cleavage with enzymes (1972) Methods in Enzymology, 25, pp. 231-244. , C.H.W. Hirs, S.N. Timasheff, Academic Press, New York
dc.descriptionEhrenpreis, Scheraga, Observations on the analysis for thrombin and the inactivation of fibrin monomer (1957) J Biol Chem, 227, pp. 1043-1061
dc.descriptionFerreira, Rocha E Silva, Potentiation of bradykinin by dimercaptopropanol (BAL) and other inhibitors of its destroying enzyme in plasma (1962) Biochem. Pharmac., 11, pp. 1123-1128
dc.descriptionFlower, Blackwell, Anti-inflammatory steroids induce biosynthesis of a phospholipase A2 inhibitor which presents prostaglandin generation (1979) Nature, Lond., 278, pp. 456-459
dc.descriptionGiglio, Identification of N-terminal tryptophan in peptides (1977) Analyt. Biochem., 82, pp. 262-263
dc.descriptionGrabar, Williams, Méthode permettant l'étude conjuguée des propriétés électrophorétiques et immunochimiques d'un mélange de protéines. Application au sérum sanguin (1953) Biochim. biophys. Acta, 10, pp. 193-194
dc.descriptionGray, End group analysis using dansyl-chloride (1972) Methods in Enzymology, 25, pp. 121-138. , C.H. Hirs, S.N. Timasheff, Academic Press, New York, part B
dc.descriptionGutiérrez, Chaves, Bolaños, Cerdas, Rojas, Arroyo, Portilla, Neutralizacion de los efectos locales del veneno de Bothrops asper por un antiveneno polivalente (1981) Toxicon, 19, pp. 493-500
dc.descriptionGutiérrez, Ownby, Odell, Isolation of a myotoxin from Bothrops asper venom: partial characterization and action on skeletal muscle (1984) Toxicon, 22, pp. 115-128
dc.descriptionGutiérrez, Rojas, Lomonte, Gené, Cerdas, Effects of a myotoxic phospholipase A2 isolated from Bothrops asper venom on skeletal muscle sarcoplasmic reticulum (1987) Toxicon, 25, pp. 1244-1248
dc.descriptionHammarström, Leukotriene (1983) Annual Review of Biochemistry, 52, pp. 355-377
dc.descriptionHomsi-Brandeburgo, Queiroz, Santo-Neto, Rodrigues-Simioni, Giglio, Fractionation of Bothrops jararacussu snake venom: partial chemical characterization and biological activity of bothropstoxin (1988) Toxicon, 26, pp. 615-627
dc.descriptionHoussay, Classification des actions des vénins de serpents sur l'organisme animal (1930) C. R. Soc. Biol. Paris, 105, pp. 308-310
dc.descriptionHuang, Lee, Isolation and pharmacological properties of phospholipase A2 from Vipera russelli (Russell's viper) snake venom (1984) Toxicon, 22, pp. 207-217
dc.descriptionItzhaki, Gill, A micro-biuret method for estimating proteins (1964) Analyt. Biochem., 9, pp. 401-410
dc.descriptionMandelbaum, Reichl, Assakura, Isolation and characterization of a proteolytic enzyme from the venom of the snake Bothrops jararaca (1982) Toxicon, 20, pp. 955-972
dc.descriptionMandelbaum, Assakura, Reichl, Characterization of two hemorrhagic factors isolated from the vemon of Bothrops neuwiedi (jararaca pintada) (1984) Toxicon, 22, pp. 193-206
dc.descriptionMebs, Samejima, Isolation and characterization of myotoxic phospholipases A2 from crotalid venoms (1986) Toxicon, 24, pp. 161-168
dc.descriptionPenke, Ferenczi, Kovacs, A new acid hydrolysis method for determining tryptophan in peptides and proteins (1974) Analyt. Biochem., 60, pp. 45-50
dc.descriptionQueiroz, Santo Neto, Rodrigues-Simioni, Franceschi, Muscle necrosis and regeneration after envenomation by Bothrops jararacussu snake venom (1984) Toxicon, 22, pp. 339-346
dc.descriptionQueiroz, Santo Neto, Assakura, Reichl, Mandelbaum, Pathological changes in muscle caused by hemorrhagic and proteolytic factors from Bothrops jararaca snake venom (1985) Toxicon, 23, pp. 341-345
dc.descriptionQueiroz, Santo Neto, Assakura, Reichl, Mandelbaum, Muscular lesions induced by a haemorrhagic factor from Bothrops neuwiedi snake venom (1985) Brazilian J. Med. Biol. Res., 18, pp. 337-340
dc.descriptionReimerdes, Klostermeyer, Determination of proteolytic activities on casein substrates (1976) Methods in Enzymology, 45, pp. 26-28. , L. Lorand, Academic Press, New York
dc.descriptionRosenfeld, Symptomatology, pathology and treatment of snake bites in South America (1971) Venomous Animals and Their Venoms, 2, pp. 345-384. , W. Bücherl, E.E. Buckley, V. Deulofeu, Academic Press, New York
dc.descriptionRothschild, Rothschild, Liberation of pharmacologically active substances by snake venoms (1979) Handbook of Experimental Pharmacology, 52, pp. 591-628. , C.Y. Lee, Springer, New York
dc.descriptionSalmon, Simmons, Moncada, The effects of BW755C and other anti-inflammatory drugs on eicosanoid concentrations and leukocyte accumulation in experimentally-induced acute inflammation (1983) J. Pharm. Pharmac., 35, pp. 808-813
dc.descriptionSampaio, Laure, Giglio, Isolation and characterization of toxic proteins from the venom of the Brazilian scorpion Tityus serrulatus (1983) Toxicon, 21, pp. 265-277
dc.descriptionSamuelsson, Leukotrienes: mediators of immediate hypersensibility reactions and inflammation (1983) Science, 220, pp. 568-575
dc.descriptionSchaeffer, Jr, Carlson, Weil, The effects of antivenin and corticosteroid analogs on rattlesnake venom shock in the rat (1979) J. Pharmac. exp. Ther., 211, pp. 409-414
dc.descriptionSchram, Moore, Bigwood, Chromatographic determination of cystine as cysteic acid (1954) Biochem. J., 57, pp. 33-37
dc.descriptionSelistre, Giglio, Isolation and characterization of a thrombin-like enzyme from the venom of the snake Bothrops insularis (jararaca ilhoa) (1987) Toxicon, 25, pp. 1135-1144
dc.descriptionShapiro, Vinuela, Matzel, Molecular weight estimation of polypeptide chains by electrophoresis in SDS polyacrylamide gels (1967) Biochem. Biophys. Res. Commun., 28, pp. 815-820
dc.descriptionSpackman, Stein, Moore, Automatic recording apparatus for use in the chromatography of amino acids (1958) Analyt. Chem., 30, pp. 1190-1206
dc.descriptionVital Brazil, Peçonhas (1982) Farmacodinâmica, pp. 1044-1074. , C.E. Corbertt, Guanabara, Rio de Janeiro
dc.descriptionWillemse, Individual variation in snake venom (1978) Comp. Biochem. Physiol., 61 B, pp. 553-557
dc.descriptionWinder, Wax, Been, Rapid foot volume measurement of unanesthetized rats and the question on the phenylbutazone effect on anaphylactoid edema (1957) Archs. int. Pharmacodyn. Ther., 112, pp. 174-187
dc.languageen
dc.publisher
dc.relationToxicon
dc.rightsfechado
dc.sourceScopus
dc.titleIsolation And Characterization Of Hemorrhagic, Myonecrotic And Edema-inducing Toxins From Bothrops Insularis (jararaca Ilhoa) Snake Venom
dc.typeArtículos de revistas


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