dc.creatorGodinho O.E.S.
dc.creatorAleixo L.M.
dc.creatorHora Alves J.P.
dc.date1982
dc.date2015-06-30T13:43:58Z
dc.date2015-11-26T14:39:08Z
dc.date2015-06-30T13:43:58Z
dc.date2015-11-26T14:39:08Z
dc.date.accessioned2018-03-28T21:44:37Z
dc.date.available2018-03-28T21:44:37Z
dc.identifier
dc.identifierAnalytical Biochemistry. , v. 123, n. 2, p. 244 - 248, 1982.
dc.identifier32697
dc.identifier10.1016/0003-2697(82)90441-9
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0020160131&partnerID=40&md5=bd93482829fd2504d700b4e2b48396c4
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/98736
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/98736
dc.identifier2-s2.0-0020160131
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1249871
dc.descriptionThe potentiometric acid-base titration curve of fully protonated lysozyme at ionic strengths of 0.10 and 1.0 m has been performed. The stoichiometry and the pKa values of each titratable group have been determined through the linearization of titration curves. Two types of carboxylic groups with pKa values of 3.76 and 5.02, the imidazole group with pKa 7.37 and the amine group with pKa 9.63, have been identified at an ionic strength of 0.10 m at 25.0°C. The number of titratable groups found per mole of protein has been 5.12 and 5.60 for the two types of carboxylic groups, 1.13 for the imidazole group, and 3.19 for the amino groups. The endpoint of the titration of the protein obtained by this method accords quite well with the endpoint obtained by the use of Gran function applied to the excess of strong base. © 1982.
dc.description123
dc.description2
dc.description244
dc.description248
dc.descriptionGodinho, Aleixo, (1981) Anal. Biochem, 112, pp. 323-328
dc.descriptionCannan, Kibrick, Palmer, THE AMPHOTERIC PROPERTIES OF EGG ALBUMIN (1941) Annals of the New York Academy of Sciences, 41, pp. 243-266
dc.descriptionTanford, Wagner, (1954) J. Amer. Chem. Soc, 76, pp. 3331-3336
dc.descriptionBeichok, Warner, Denaturation and Electrophoretic Behavior of Lysozyme1,2 (1959) Journal of the American Chemical Society, 81, pp. 1892-1897
dc.descriptionPehrsson, Ingman, Axel, (1976) Talanta, 23, pp. 769-780
dc.descriptionGran, (1952) Analyst (London), 77, pp. 661-671
dc.descriptionCanfield, (1963) J. Biol. Chem, 238, pp. 2698-2707
dc.descriptionNozaki, Tanford, (1967) J. Biol. Chem, 242, pp. 4731-4735
dc.descriptionGodinho, O. E. S., Aleixo, L. M., and Hora Alves, J. P, paper to be published
dc.languageen
dc.publisher
dc.relationAnalytical Biochemistry
dc.rightsfechado
dc.sourceScopus
dc.titleCharacterization And Determination Of Titratable Groups Of Proteins By Linearization Of Titration Curves. Ii. Application To Lysozyme
dc.typeArtículos de revistas


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