dc.creator | Elton J.R.V. | |
dc.creator | Vinicius S.N. | |
dc.creator | Marcelo S.D.S. | |
dc.creator | Marcela S. | |
dc.creator | Peter J.M. | |
dc.creator | Maria I.N.C. | |
dc.date | 2014 | |
dc.date | 2015-06-25T17:54:58Z | |
dc.date | 2015-11-26T14:37:13Z | |
dc.date | 2015-06-25T17:54:58Z | |
dc.date | 2015-11-26T14:37:13Z | |
dc.date.accessioned | 2018-03-28T21:41:32Z | |
dc.date.available | 2018-03-28T21:41:32Z | |
dc.identifier | | |
dc.identifier | Plos One. Public Library Of Science, v. 9, n. 11, p. - , 2014. | |
dc.identifier | 19326203 | |
dc.identifier | 10.1371/journal.pone.0112061 | |
dc.identifier | http://www.scopus.com/inward/record.url?eid=2-s2.0-84911490342&partnerID=40&md5=e72af47489da811dd47b4117e1287ebe | |
dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/86764 | |
dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/86764 | |
dc.identifier | 2-s2.0-84911490342 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1249072 | |
dc.description | Telomerase RNAs (TERs) are highly divergent between species, varying in size and sequence composition. Here, we identify a candidate for the telomerase RNA component of Leishmania genus, which includes species that cause leishmaniasis, a neglected tropical disease. Merging a thorough computational screening combined with RNA-seq evidence, we mapped a non-coding RNA gene localized in a syntenic locus on chromosome 25 of five Leishmania species that shares partial synteny with both Trypanosoma brucei TER locus and a putative TER candidate-containing locus of Crithidia fasciculata . Using targetdriven molecular biology approaches, we detected a ∼ 2,100 nt transcript (LeishTER) that contains a 5′ spliced leader (SL) cap, a putative 3′ polyA tail and a predicted C/D box snoRNA domain. LeishTER is expressed at similar levels in the logarithmic and stationary growth phases of promastigote forms. A 5′SL capped LeishTER co-immunoprecipitated and colocalized with the telomerase protein component (TERT) in a cell cycle-dependent manner. Prediction of its secondary structure strongly suggests the existence of a bona fide single-stranded template sequence and a conserved C[U/C]GUCA motif-containing helix II, representing the template boundary element. This study paves the way for further investigations on the biogenesis of parasite TERT ribonucleoproteins (RNPs) and its role in parasite telomere biology. | |
dc.description | 9 | |
dc.description | 11 | |
dc.description | | |
dc.description | | |
dc.description | Vickerman, K., Preston, T.M., Spindle microtubules in the dividing nuclei of trypanosomes (1970) J Cell Sci, 6, pp. 365-383 | |
dc.description | Solari, A.J., The 3-dimensional fine structure of the mitotic spindle in Trypanosoma cruzi (1980) Chromosoma, 78, pp. 239-255 | |
dc.description | Cano, M.I., Telomere biology of trypanosomatids: More questions than answers (2001) Trends Parasitol, 17, pp. 425-429 | |
dc.description | Blackburn, E.H., Challoner, P.B., Identification of a telomeric DNA sequence in Trypanosoma brucei (1984) Cell, 36, pp. 447-457 | |
dc.description | Fu, G., Barker, D.C., Characterisation of Leishmania telomeres reveals unusual telomeric repeats and conserved telomere-associated sequence (1998) Nucleic Acids Res, 26, pp. 2161-2167 | |
dc.description | Conte, F.F., Cano, M.I., Genomic organization of telomeric and subtelomeric sequences of Leishmania (Leishmania) amazonensis (2005) Int J Parasitol, 35, pp. 1435-1443 | |
dc.description | Yu, G.L., Bradley, J.D., Attardi, L.D., Blackburn, E.H., In vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAs (1990) Nature, 344, pp. 126-132 | |
dc.description | Feng, J., Funk, W.D., Wang, S.S., Weinrich, S.L., Avilion, A.A., The RNA component of human telomerase (1995) Science, 269, pp. 1236-1241 | |
dc.description | Cano, M.I., Dungan, J.M., Agabian, N., Blackburn, E.H., Telomerase in kinetoplastid parasitic protozoa (1999) Proc Natl Acad Sci U S A, 96, pp. 3616-3621 | |
dc.description | Munoz, D.P., Collins, K., Biochemical properties of Trypanosoma cruzi telomerase (2004) Nucleic Acids Res, 32, pp. 5214-5222 | |
dc.description | Giardini, M.A., Fernandez, M.F., Lira, C.B., Cano, M.I., Leishmania amazonensis: Partial purification and study of the biochemical properties of the telomerase reverse transcriptase activity from promastigote-stage (2011) Exp Parasitol, 127, pp. 243-248 | |
dc.description | Giardini, M.A., Lira, C.B., Conte, F.F., Camillo, L.R., De Siqueira Neto, J.L., The putative telomerase reverse transcriptase component of Leishmania amazonensis: Gene cloning and characterization (2006) Parasitol Res, 98, pp. 447-454 | |
dc.description | Sykorova, E., Fajkus, J., Structure-function relationships in telomerase genes (2009) Biol Cell, 101, pp. 375-392. , 371 p following 392 | |
dc.description | Xia, J., Peng, Y., Mian, I.S., Lue, N.F., Identification of functionally important domains in the N-terminal region of telomerase reverse transcriptase (2000) Mol Cell Biol, 20, pp. 5196-5207 | |
dc.description | Armbruster, B.N., Banik, S.S., Guo, C., Smith, A.C., Counter, C.M., N-terminal domains of the human telomerase catalytic subunit required for enzyme activity in vivo (2001) Mol Cell Biol, 21, pp. 7775-7786 | |
dc.description | Lai, C.K., Mitchell, J.R., Collins, K., RNA binding domain of telomerase reverse transcriptase (2001) Mol Cell Biol, 21, pp. 990-1000 | |
dc.description | Lingner, J., Hughes, T.R., Shevchenko, A., Mann, M., Lundblad, V., Reverse transcriptase motifs in the catalytic subunit of telomerase (1997) Science, 276, pp. 561-567 | |
dc.description | Nakamura, T.M., Morin, G.B., Chapman, K.B., Weinrich, S.L., Andrews, W.H., Telomerase catalytic subunit homologs from fission yeast and human (1997) Science, 277, pp. 955-959 | |
dc.description | Weinrich, S.L., Pruzan, R., Ma, L., Ouellette, M., Tesmer, V.M., Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT (1997) Nat Genet, 17, pp. 498-502 | |
dc.description | Counter, C.M., Meyerson, M., Eaton, E.N., Weinberg, R.A., The catalytic subunit of yeast telomerase (1997) Proc Natl Acad Sci U S A, 94, pp. 9202-9207 | |
dc.description | Sandhu, R., Sanford, S., Basu, S., Park, M., Pandya, U.M., A trans-spliced telomerase RNA dictates telomere synthesis in Trypanosoma brucei (2013) Cell Res, 23, pp. 537-551 | |
dc.description | Gupta, S.K., Kolet, L., Doniger, T., Biswas, V.K., Unger, R., The Trypanosoma brucei telomerase RNA (TER) homologue binds core proteins of the C/D snoRNA family (2013) FEBS Lett, 587, pp. 1399-1404 | |
dc.description | Theimer, C.A., Feigon, J., Structure and function of telomerase RNA (2006) Curr Opin Struct Biol, 16, pp. 307-318 | |
dc.description | Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Gapped BLAST and PSI-BLAST: A new generation of protein database search programs (1997) Nucleic Acids Res, 25, pp. 3389-3402 | |
dc.description | Chiurillo, M.A., Beck, A.E., Devos, T., Myler, P.J., Stuart, K., Cloning and characterization of Leishmania donovani telomeres (2000) Exp Parasitol, 94, pp. 248-258 | |
dc.description | Carver, T.J., Rutherford, K.M., Berriman, M., Rajandream, M.A., Barrell, B.G., ACT: The artemis comparison tool (2005) Bioinformatics, 21, pp. 3422-3423 | |
dc.description | Thompson, J.D., Higgins, D.G., Gibson, T.J., CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice (1994) Nucleic Acids Res, 22, pp. 4673-4680 | |
dc.description | Waterhouse, A.M., Procter, J.B., Martin, D.M., Clamp, M., Barton, G.J., Jalview Version 2-A multiple sequence alignment editor and analysis workbench (2009) Bioinformatics, 25, pp. 1189-1191 | |
dc.description | Bernhart, S.H., Hofacker, I.L., Will, S., Gruber, A.R., Stadler, P.F., RNAalifold: Improved consensus structure prediction for RNA alignments (2008) BMC Bioinformatics, 9, p. 474 | |
dc.description | Zuker, M., Mfold web server for nucleic acid folding and hybridization prediction (2003) Nucleic Acids Res, 31, pp. 3406-3415 | |
dc.description | Rivas, E., Eddy, S.R., A dynamic programming algorithm for RNA structure prediction including pseudoknots (1999) J Mol Biol, 285, pp. 2053-2068 | |
dc.description | Sperschneider, J., Datta, A., KnotSeeker: Heuristic pseudoknot detection in long RNA sequences (2008) Rna, 14, pp. 630-640 | |
dc.description | Harmanci, A.O., Sharma, G., Mathews, D.H., TurboFold: Iterative probabilistic estimation of secondary structures for multiple RNA sequences (2011) BMC Bioinformatics, 12, p. 108 | |
dc.description | Kapler, G.M., Coburn, C.M., Beverley, S.M., Stable transfection of the human parasite Leishmania major delineates a 30-kilobase region sufficient for extrachromosomal replication and expression (1990) Mol Cell Biol, 10, pp. 1084-1094 | |
dc.description | Ambit, A., Woods, K.L., Cull, B., Coombs, G.H., Mottram, J.C., Morphological events during the cell cycle of Leishmania major (2011) Eukaryot Cell, 10, pp. 1429-1438 | |
dc.description | Chiurillo, M.A., Ramirez, J.L., Charaterization of Leishmania major Friedlin telomeric terminus (2002) Mem Inst Oswaldo Cruz, 97, pp. 343-346 | |
dc.description | Fernandes, A.P., Nelson, K., Beverley, S.M., Evolution of nuclear ribosomal RNAs in kinetoplastid protozoa: Perspectives on the age and origins of parasitism (1993) Proc Natl Acad Sci U S A, 90, pp. 11608-11612 | |
dc.description | Jackson, A.P., The evolution of amastin surface glycoproteins in trypanosomatid parasites (2010) Mol Biol Evol, 27, pp. 33-45 | |
dc.description | Cros, M.J., De Monte, A., Mariette, J., Bardou, P., Grenier-Boley, B., RNAspace.Org: An integrated environment for the prediction, annotation, and analysis of ncRNA (2011) Rna, 17, pp. 1947-1956 | |
dc.description | Darzacq, X., Jady, B.E., Verheggen, C., Kiss, A.M., Bertrand, E., Cajal body-specific small nuclear RNAs: A novel class of 2'-O-methylation and pseudouridylation guide RNAs (2002) Embo J, 21, pp. 2746-2756 | |
dc.description | Kolev, N.G., Franklin, J.B., Carmi, S., Shi, H., Michaeli, S., The transcriptome of the human pathogen Trypanosoma brucei at single-nucleotide resolution (2010) PLoS Pathog, 6, p. e1001090 | |
dc.description | Bianchi, A., Shore, D., How telomerase reaches its end: Mechanism of telomerase regulation by the telomeric complex (2008) Mol Cell, 31, pp. 153-165 | |
dc.description | Mattick, J.S., The genetic signatures of noncoding RNAs (2009) PLoS Genet, 5, p. e1000459 | |
dc.description | Romero, D.P., Blackburn, E.H., A conserved secondary structure for telomerase RNA (1991) Cell, 67, pp. 343-353 | |
dc.description | Greider, C.W., Blackburn, E.H., A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis (1989) Nature, 337, pp. 331-337 | |
dc.description | El-Sayed, N.M., Myler, P.J., Blandin, G., Berriman, M., Crabtree, J., Comparative genomics of trypanosomatid parasitic protozoa (2005) Science, 309, pp. 404-409 | |
dc.description | Longhese, M.P., Bonetti, D., Manfrini, N., Clerici, M., Mechanisms and regulation of DNA end resection (2010) Embo J, 29, pp. 2864-2874 | |
dc.description | Laurentino, E.C., Ruiz, J.C., Fazelinia, G., Myler, P.J., Degrave, W., A survey of Leishmania braziliensis genome by shotgun sequencing (2004) Molecular and Biochemical Parasitology, 137, pp. 81-86 | |
dc.description | Vasconcelos, E.J., Terrao, M.C., Ruiz, J.C., Vencio, R.Z., Cruz, A.K., In silico identification of conserved intercoding sequences in Leishmania genomes: Unraveling putative cis-regulatory elements (2012) Mol Biochem Parasitol, 183, pp. 140-150 | |
dc.description | Rastrojo, A., Carrasco-Ramiro, F., Martin, D., Crespillo, A., Reguera, R.M., The transcriptome of Leishmania major in the axenic promastigote stage: Transcript annotation and relative expression levels by RNA-seq (2013) BMC Genomics, 14, p. 223 | |
dc.description | Chakrabarti, K., Pearson, M., Grate, L., Sterne-Weiler, T., Deans, J., Structural RNAs of known and unknown function identified in malaria parasites by comparative genomics and RNA analysis (2007) Rna, 13, pp. 1923-1939 | |
dc.description | Hukezalie, K.R., Wong, J.M., Structure-function relationship and biogenesis regulation of the human telomerase holoenzyme (2013) Febs J, 280, pp. 3194-3204 | |
dc.description | Kachouri-Lafond, R., Dujon, B., Gilson, E., Westhof, E., Fairhead, C., Large telomerase RNA, telomere length heterogeneity and escape from senescence in Candida glabrata (2009) Febs Lett, 583, pp. 3605-3610 | |
dc.description | Muhich, M.L., Boothroyd, J.C., Polycistronic transcripts in trypanosomes and their accumulation during heat shock: Evidence for a precursor role in mRNA synthesis (1988) Mol Cell Biol, 8, pp. 3837-3846 | |
dc.description | Huang, J., Van Der Ploeg, L.H., Maturation of polycistronic pre-mRNA in Trypanosoma brucei: Analysis of trans splicing and poly(A) addition at nascent RNA transcripts from the hsp70 locus (1991) Mol Cell Biol, 11, pp. 3180-3190 | |
dc.description | Mitchell, J.R., Cheng, J., Collins, K., A box H/ACA small nucleolar RNA-like domain at the human telomerase RNA 3' end (1999) Mol Cell Biol, 19, pp. 567-576 | |
dc.description | Fu, D., Collins, K., Distinct biogenesis pathways for human telomerase RNA and H/ACA small nucleolar RNAs (2003) Mol Cell, 11, pp. 1361-1372 | |
dc.description | Leonardi, J., Box, J.A., Bunch, J.T., Baumann, P., TER1, the RNA subunit of fission yeast telomerase (2008) Nat Struct Mol Biol, 15, pp. 26-33 | |
dc.description | Egan, E.D., Collins, K., Specificity and stoichiometry of subunit interactions in the human telomerase holoenzyme assembled in vivo (2010) Mol Cell Biol, 30, pp. 2775-2786 | |
dc.description | Noel, J.F., Larose, S., Abou Elela, S., Wellinger, R.J., Budding yeast telomerase RNA transcription termination is dictated by the Nrd1/Nab3 non-coding RNA termination pathway (2012) Nucleic Acids Res, 40, pp. 5625-5636 | |
dc.description | Egan, E.D., Collins, K., Biogenesis of telomerase ribonucleoproteins (2012) Rna, 18, pp. 1747-1759 | |
dc.description | Wilusz, J.E., Spector, D.L., An unexpected ending: Noncanonical 3' end processing mechanisms (2010) Rna, 16, pp. 259-266 | |
dc.description | Chapon, C., Cech, T.R., Zaug, A.J., Polyadenylation of telomerase RNA in budding yeast (1997) Rna, 3, pp. 1337-1351 | |
dc.description | Chen, J.L., Greider, C.W., An emerging consensus for telomerase RNA structure (2004) Proc Natl Acad Sci U S A, 101, pp. 14683-14684 | |
dc.description | Proctor, J.R., Meyer, I.M., COFOLD: An RNA secondary structure prediction method that takes co-transcriptional folding into account (2013) Nucleic Acids Res, 41, p. e102 | |
dc.description | Autexier, C., Greider, C.W., Boundary elements of the Tetrahymena telomerase RNA template and alignment domains (1995) Genes Dev, 9, pp. 2227-2239 | |
dc.description | Lai, C.K., Miller, M.C., Collins, K., Template boundary definition in Tetrahymena telomerase (2002) Genes Dev, 16, pp. 415-420 | |
dc.description | Richards, R.J., Theimer, C.A., Finger, L.D., Feigon, J., Structure of the Tetrahymena thermophila telomerase RNA helix II template boundary element (2006) Nucleic Acids Res, 34, pp. 816-825 | |
dc.description | Requena, J.M., Lights and shadows on gene organization and regulation of gene expression in Leishmania (2011) Front Biosci, 16, pp. 2069-2085. , (Landmark Ed) | |
dc.description | De Rijk, P., Wuyts, J., De Wachter, R., RnaViz 2: An improved representation of RNA secondary structure (2003) Bioinformatics, 19, pp. 299-300 | |
dc.language | en | |
dc.publisher | Public Library of Science | |
dc.relation | PLoS ONE | |
dc.rights | aberto | |
dc.source | Scopus | |
dc.title | The Putative Leishmania Telomerase Rna (leishter) Undergoes Trans-splicing And Contains A Conserved Template Sequence | |
dc.type | Artículos de revistas | |