Artículos de revistas
Integrated Proteomics Identified Up-regulated Focal Adhesion-mediated Proteins In Human Squamous Cell Carcinoma In An Orthotopic Murine Model
Registro en:
Plos One. Public Library Of Science, v. 9, n. 5, p. - , 2014.
19326203
10.1371/journal.pone.0098208
2-s2.0-84901473166
Autor
Granato D.C.
Zanetti M.R.
Kawahara R.
Yokoo S.
Domingues R.R.
Aragao A.Z.
Agostini M.
Carazzolle M.F.
Vidal R.O.
Flores I.L.
Korvala J.
Cervigne N.K.
Silva A.R.S.
Coletta R.D.
Graner E.
Sherman N.E.
Paes Leme A.F.
Institución
Resumen
Understanding the molecular mechanisms of oral carcinogenesis will yield important advances in diagnostics, prognostics, effective treatment, and outcome of oral cancer. Hence, in this study we have investigated the proteomic and peptidomic profiles by combining an orthotopic murine model of oral squamous cell carcinoma (OSCC), mass spectrometry-based proteomics and biological network analysis. Our results indicated the up-regulation of proteins involved in actin cytoskeleton organization and cell-cell junction assembly events and their expression was validated in human OSCC tissues. In addition, the functional relevance of talin-1 in OSCC adhesion, migration and invasion was demonstrated. Taken together, this study identified specific processes deregulated in oral cancer and provided novel refined OSCC-targeting molecules. © 2014 Granato et al. 9 5
Siegel, R., Naishadham, D., Jemal, A., Cancer statistics, 2013 (2013) CA Cancer J Clin, 63, pp. 11-30 Wong, D.T.W., Todd, R., Tsuji, T., Donoff, R.B., Molecular biology of human oral cancer (1996) Critical Reviews in Oral Biology and Medicine, 7 (4), pp. 319-328 Warnakulasuriya, S., Global epidemiology of oral and oropharyngeal cancer (2009) Oral Oncol, 45, pp. 309-316 Choi, S., Myers, J.N., Molecular pathogenesis of oral squamous cell carcinoma: Implications for therapy (2008) J Dent Res, 87, pp. 14-32 Panis, C., Pizzatti, L., Herrera, A.C., Cecchini, R., Abdelhay, E., Putative circulating markers of the early and advanced stages of breast cancer identified by high-resolution label-free proteomics (2013) Cancer Lett, 330, pp. 57-66 Fujita, Y., Nakanishi, T., Miyamoto, Y., Hiramatsu, M., Mabuchi, H., Proteomics-based identification of autoantibody against heat shock protein 70 as a diagnostic marker in esophageal squamous cell carcinoma (2008) Cancer Lett, 263, pp. 280-290 Mognetti, B., Di Carlo, F., Berta, G.N., Animal models in oral cancer research (2006) Oral Oncol, 42, pp. 448-460 Hwang, Y.S., Zhang, X., Park, K.K., Chung, W.Y., An orthotopic and osteolytic model with a newly established oral squamous cell carcinoma cell line (2012) Arch Oral Biol, 58, pp. 218-225 Boukamp, P., Petrussevska, R.T., Breitkreutz, D., Hornung, J., Markham, A., Fusenig, N.E., Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line (1988) Journal of Cell Biology, 106 (3), pp. 761-771 Agostini, M., Almeida, L.Y., Bastos, D.C., Ortega, R.M., Moreira, F.S., The fatty acid synthase inhibitor orlistat reduces the growth and metastasis of orthotopic tongue oral squamous cell carcinomas (2014) Mol Cancer Ther, 13, pp. 585-595 Villen, J., Gygi, S.P., The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry (2008) Nat Protoc, 3, pp. 1630-1638 Paes Leme, A.F., Sherman, N.E., Smalley, D.M., Sizukusa, L.O., Oliveira, A.K., Hemorrhagic activity of HF3, a snake venom metalloproteinase: Insights from the proteomic analysis of mouse skin and blood plasma (2012) J Proteome Res, 11, pp. 279-291 Aragao, A.Z., Nogueira, M.L., Granato, D.C., Simabuco, F.M., Honorato, R.V., Identification of novel interaction between ADAM17 (a disintegrin and metalloprotease 17) and thioredoxin-1 (2012) J Biol Chem, 287, pp. 43071-43082 (2009) R: A Language and Environment for Statistical Computing. 2.11.1 Ed., , Team RDC Vienna: R Foundation for Statistical Computing Zhang, B., VerBerkmoes, N.C., Langston, M.A., Uberbacher, E., Hettich, R.L., Samatova, N.F., Detecting differential and correlated protein expression in label-free shotgun proteomics (2006) Journal of Proteome Research, 5 (11), pp. 2909-2918. , DOI 10.1021/pr0600273 Cox, J., Mann, M., MaxQuant enables high peptide identification rates, individualized p.P.B.-range mass accuracies and proteome-wide protein quantification (2008) Nat Biotechnol, 26, pp. 1367-1372 Kanehisa, M., Araki, M., Goto, S., Hattori, M., Hirakawa, M., Itoh, M., Katayama, T., Yamanishi, Y., KEGG for linking genomes to life and the environment (2008) Nucleic Acids Research, 36 (SUPPL. 1), pp. D480-D484. , DOI 10.1093/nar/gkm882 Dennis Jr., G., Sherman, B.T., Hosack, D.A., Yang, J., Gao, W., DAVID: Database for Annotation, Visualization, and Integrated Discovery (2003) Genome Biol, 4, pp. P3 Wang, Z., Jiang, L., Huang, C., Li, Z., Chen, L., Comparative proteomics approach to screening of potential diagnostic and therapeutic targets for oral squamous cell carcinoma (2008) Mol Cell Proteomics, 7, pp. 1639-1650 Wetting, H.L., Hadler-Olsen, E., Magnussen, S., Rikardsen, O., Steigen, S.E., S100A4 expression in xenograft tumors of human carcinoma cell lines is induced by the tumor microenvironment (2011) Am J Pathol, 178, pp. 2389-2396 Myers, J.N., Holsinger, F.C., Jasser, S.A., Bekele, B.N., Fidler, I.J., An orthotopic nude mouse model of oral tongue squamous cell carcinoma (2002) Clinical Cancer Research, 8 (1), pp. 293-298 Heath, C.H., Deep, N.L., Sweeny, L., Zinn, K.R., Rosenthal, E.L., Use of panitumumab-IRDye800 to image microscopic head and neck cancer in an orthotopic surgical model (2012) Ann Surg Oncol, 19, pp. 3879-3887 Wang, Y., McNiven, M.A., Invasive matrix degradation at focal adhesions occurs via protease recruitment by a FAK-p130Cas complex (2012) J Cell Biol, 196, pp. 375-385 Lai, M.T., Hua, C.H., Tsai, M.H., Wan, L., Lin, Y.J., Talin-1 overexpression defines high risk for aggressive oral squamous cell carcinoma and promotes cancer metastasis (2011) J Pathol, 224, pp. 367-376 Drees, F., Pokutta, S., Yamada, S., Nelson, W.J., Weis, W.I., Alpha-catenin is a molecular switch that binds E-cadherin-beta-catenin and regulates actin-filament assembly (2005) Cell, 123 (5), pp. 903-915. , DOI 10.1016/j.cell.2005.09.021, PII S009286740500975X Kiema, T., Lad, Y., Jiang, P., Oxley, C.L., Baldassarre, M., Wegener, K.L., Campbell, I.D., Calderwood, D.A., The molecular basis of filamin binding to integrins and competition with talin (2006) Molecular Cell, 21 (3), pp. 337-347. , DOI 10.1016/j.molcel.2006.01.011, PII S1097276506000311 Yue, J., Lu, H., Liu, J., Berwick, M., Shen, Z., Filamin-A as a marker and target for DNA damage based cancer therapy (2012) DNA Repair (Amst), 11, pp. 192-200 Warburg, O., On the origin of cancer cells (1956) Science, 123, pp. 309-314 Jones, R.G., Thompson, C.B., Tumor suppressors and cell metabolism: A recipe for cancer growth (2009) Genes Dev, 23, pp. 537-548 Hanahan, D., Weinberg, R.A., Hallmarks of cancer: The next generation (2011) Cell, 144, pp. 646-674 Israel, M., Schwartz, L., The metabolic advantage of tumor cells (2011) Mol Cancer, 10, p. 70 Cantor, J.R., Sabatini, D.M., Cancer cell metabolism: One hallmark, many faces (2012) Cancer Discov, 2, pp. 881-898 Shen, Y., Tolic, N., Xie, F., Zhao, R., Purvine, S.O., Effectiveness of CID, HCD, and ETD with FT MS/MS for degradomic- peptidomic analysis: Comparison of peptide identification methods (2011) J Proteome Res, 10, pp. 3929-3943 Assinder, S.J., Stanton, J.A., Prasad, P.D., Transgelin: An actin-binding protein and tumour suppressor (2009) Int J Biochem Cell Biol, 41, pp. 482-486 Lynch, C.D., Lazar, A.M., Iskratsch, T., Zhang, X., Sheetz, M.P., Endoplasmic spreading requires coalescence of vimentin intermediate filaments at force-bearing adhesions (2013) Mol Biol Cell, 24, pp. 21-30 Gelman, J.S., Sironi, J., Castro, L.M., Ferro, E.S., Fricker, L.D., Peptidomic analysis of human cell lines (2011) J Proteome Res, 10, pp. 1583-1592 Findeisen, P., Neumaier, M., Functional protease profiling for diagnosis of malignant disease (2012) Proteomics Clin Appl, 6, pp. 60-78 Doucet, A., Butler, G.S., Rodriguez, D., Prudova, A., Overall, C.M., Metadegradomics: Toward in vivo quantitative degradomics of proteolytic post-translational modifications of the cancer proteome (2008) Mol Cell Proteomics, 7, pp. 1925-1951