Artículos de revistas
Overlapped Sequence Types (sts) And Serogroups Of Avian Pathogenic (apec) And Human Extra-intestinal Pathogenic (expec) Escherichia Coli Isolated In Brazil
Registro en:
Plos One. Public Library Of Science, v. 9, n. 8, p. - , 2014.
19326203
10.1371/journal.pone.0105016
2-s2.0-84905833533
Autor
Maluta R.P.
Logue C.M.
Casas M.R.T.
Meng T.
Guastalli E.A.L.
Rojas T.C.G.
Montelli A.C.
Sadatsune T.
Ramos M.D.C.
Nolan L.K.
Silveira W.D.D.
Institución
Resumen
Avian pathogenic Escherichia coli (APEC) strains belong to a category that is associated with colibacillosis, a serious illness in the poultry industry worldwide. Additionally, some APEC groups have recently been described as potential zoonotic agents. In this work, we compared APEC strains with extraintestinal pathogenic E. coli (ExPEC) strains isolated from clinical cases of humans with extra-intestinal diseases such as urinary tract infections (UTI) and bacteremia. PCR results showed that genes usually found in the ColV plasmid (tsh, iucA, iss, and hlyF) were associated with APEC strains while fyuA, irp-2, fepC sitDchrom, fimH, crl, csgA, afa, iha, sat, hlyA, hra, cnf1, kpsMTII, clpVSakai and malX were associated with human ExPEC. Both categories shared nine serogroups (O2, O6, O7, O8, O11, O19, O25, O73 and O153) and seven sequence types (ST10, ST88, ST93, ST117, ST131, ST155, ST359, ST648 and ST1011). Interestingly, ST95, which is associated with the zoonotic potential of APEC and is spread in avian E. coli of North America and Europe, was not detected among 76 APEC strains. When the strains were clustered based on the presence of virulence genes, most ExPEC strains (71.7%) were contained in one cluster while most APEC strains (63.2%) segregated to another. In general, the strains showed distinct genetic and fingerprint patterns, but avian and human strains of ST359, or ST23 clonal complex (CC), presented more than 70% of similarity by PFGE. The results demonstrate that some "zoonotic-related" STs (ST117, ST131, ST10CC, ST23CC) are present in Brazil. Also, the presence of moderate fingerprint similarities between ST359 E. coli of avian and human origin indicates that strains of this ST are candidates for having zoonotic potential. © 2014 Maluta et al. 9 8
Dziva, F., Stevens, M.P., Colibacillosis in poultry: Unravelling the molecular basis of virulence of avian pathogenic Escherichia coli in their natural hosts (2008) Avian Pathology, 37 (4), pp. 355-366. , DOI 10.1080/03079450802216652, PII 794893305 Ewers, C., Li, G., Wilking, H., Kiessling, S., Alt, K., Antao E.-M, Laturnus, C., Wieler, L.H., Avian pathogenic, uropathogenic, and newborn meningitis-causing Escherichia coli: How closely related are they? (2007) International Journal of Medical Microbiology, 297 (3), pp. 163-176. , DOI 10.1016/j.ijmm.2007.01.003, PII S1438422107000173 Moulin-Schouleur, M., Reperant, M., Laurent, S., Bree, A., Mignon-Grasteau, S., Germon, P., Rasschaert, D., Schouler, C., Extraintestinal pathogenic Escherichia coli strains of avian and human origin: Link between phylogenetic relationships and common virulence patterns (2007) Journal of Clinical Microbiology, 45 (10), pp. 3366-3376. , DOI 10.1128/JCM.00037-07 Rodriguez-Siek, K.E., Giddings, C.W., Doetkott, C., Johnson, T.J., Fakhr, M.K., Nolan, L.K., Comparison of Escherichia coli isolates implicated in human urinary tract infection and avian colibacillosis (2005) Microbiology, 151 (6), pp. 2097-2110. , DOI 10.1099/mic.0.27499-0 Mellata, M., Human and avian extraintestinal pathogenic Escherichia coli: Infections, zoonotic risks, and antibiotic resistance trends (2013) Foodborne Pathog Dis, 10, pp. 916-932 Moreno, E., Andreu, A., Perez, T., Sabate, M., Johnson, J.R., Prats, G., Relationship between Escherichia coli strains causing urinary tract infection in women and the dominant faecal flora of the same hosts (2006) Epidemiology and Infection, 134 (5), pp. 1015-1023. , DOI 10.1017/S0950268806005917, PII S0950268806005917 Yamamoto, S., Tsukamoto, T., Terai, A., Kurazono, H., Takeda, Y., Yoshida, O., Genetic evidence supporting the fecal-perineal-urethral hypothesis in cystitis caused by Escherichia coli (1997) Journal of Urology, 157 (3), pp. 1127-1129. , DOI 10.1016/S0022-5347(01)65154-1 Russo, T.A., Johnson, J.R., Proposal for a new inclusive designation for extraintestinal pathogenic isolates of Escherichia coli: ExPEC (2000) Journal of Infectious Diseases, 181 (5), pp. 1753-1754. , DOI 10.1086/315418 Kaper, J.B., Nataro, J.P., Mobley, H.L.T., Pathogenic Escherichia coli (2004) Nature Reviews Microbiology, 2 (2), pp. 123-140. , DOI 10.1038/nrmicro818 Johnson, T.J., Wannemuehler, Y., Johnson, S.J., Stell, A.L., Doetkott, C., Comparison of extraintestinal pathogenic Escherichia coli strains from human and avian sources reveals a mixed subset representing potential zoonotic pathogens (2008) Appl Environ Microbiol, 74, pp. 7043-7050 Danzeisen, J.L., Wannemuehler, Y., Nolan, L.K., Johnson, T.J., Comparison of multilocus sequence analysis and virulence genotyping of Escherichia coli from live birds, retail poultry meat, and human extraintestinal infection (2013) Avian Dis, 57, pp. 104-108 Vincent, C., Boerlin, P., Daignault, D., Dozois, C.M., Dutil, L., Food reservoir for Escherichia coli causing urinary tract infections (2010) Emerg Infect Dis, 16, pp. 88-95 Maluta, R.P., Gatti, M.S.V., Joazeiro, P.P., De Paiva, J.B., Rojas, T.C.G., Avian extra-intestinal Escherichia coliexhibits enterotoxigenic-like activity in the in vivo rabbit ligated ileal loop assay (2014) Foodborne Pathogens and Disease, 11, pp. 484-489 Arbeit, D.J., Laboratory procedures for the epidemiologic analysis of microorganisms (1995) Manual of Clinical Microbiology. 6th Ed, pp. 190-208. , Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, editors. Washington, D.C.: American Society for Microbiology Orskov, F., Orskov, I., Serotyping of Escherichia coli (1984) Methods Microbiol, 14, pp. 43-112 Machado, J., Grimont, F., Grimont, P.A., Identification of Escherichia coli flagellar types by restriction of the amplified fliC gene (2000) Res Microbiol, 151, pp. 535-546 Borges, C.A., Beraldo, L.G., Maluta, R.P., Cardozo, M.V., Guth, B.E.C., Shiga Toxigenic and Atypical Enteropathogenic Escherichia coli in the Feces and Carcasses of Slaughtered Pigs (2012) Foodborne Pathogens and Disease, 9, pp. 1119-1125 Clermont, O., Bonacorsi, S., Bingen, E., Rapid and simple determination of the Escherichia coli phylogenetic group (2000) Appl Environ Microbiol, 66, pp. 4555-4558 De Hoon, M.J.L., Imoto, S., Nolan, J., Miyano, S., Open source clustering software (2004) Bioinformatics, 20 (9), pp. 1453-1454. , DOI 10.1093/bioinformatics/bth078 Saldanha, A.J., Java Treeview-extensible visualization of microarray data (2004) Bioinformatics, 20, pp. 3246-3248 Wirth, T., Falush, D., Lan, R., Colles, F., Mensa, P., Sex and virulence in Escherichia coli: An evolutionary perspective (2006) Mol Microbiol, 60, pp. 1136-1151 Tamura, K., Nei, M., Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees (1993) Molecular Biology and Evolution, 10 (3), pp. 512-526 Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods (2011) Mol Biol Evol, 28, pp. 2731-2739 Smith, J.L., Fratamico, P.M., Gunther, N.W., Extraintestinal pathogenic Escherichia coli (2007) Foodborne Pathogens and Disease, 4 (2), pp. 134-163. , DOI 10.1089/fpd.2007.0087 Ginns, C.A., Benham, M.L., Adams, L.M., Whithear, K.G., Bettelheim, K.A., Crabb, B.S., Browning, G.F., Colonization of the respiratory tract by a virulent strain of avian Escherichia coli requires carriage of a conjugative plasmid (2000) Infection and Immunity, 68 (3), pp. 1535-1541. , DOI 10.1128/IAI.68.3.1535-1541.2000 Manges, A.R., Johnson, J.R., Food-borne origins of Escherichia coli causing extraintestinal infections (2012) Clin Infect Dis, 55, pp. 712-719 Mora, A., López, C., Herrera, A., Viso, S., Mamani, R., Emerging avian pathogenic Escherichia coli strains belonging to clonal groups O111:H4-DST2085 and O111:H4-D-ST117 with high virulence-gene content and zoonotic potential (2012) Vet Microbiol, 156, pp. 347-352 Dissanayake, D.R., Octavia, S., Lan, R., Population structure and virulence content of avian pathogenic Escherichia coli isolated from outbreaks in Sri Lanka (2014) Vet Microbiol, 168, pp. 403-412 Hussein, A.H., Ghanem, I.A., Eid, A.A., Ali, M.A., Sherwood, J.S., Molecular and phenotypic characterization of Escherichia coli isolated from broiler chicken flocks in Egypt (2013) Avian Dis, 57, pp. 602-611 Pires-dos-Santos, T., Bisgaard, M., Christensen, H., Genetic diversity and virulence profiles of Escherichia coli causing salpingitis and peritonitis in broiler breeders (2013) Vet Microbiol, 162, pp. 873-880 Giufrè, M., Graziani, C., Accogli, M., Luzzi, I., Busani, L., Escherichia coli of human and avian origin: Detection of clonal groups associated with fluoroquinolone and multidrug resistance in Italy (2012) J Antimicrob Chemother, 67, pp. 860-867 Platell, J.L., Johnson, J.R., Cobbold, R.N., Trott, D.J., Multidrug-resistant extraintestinal pathogenic Escherichia coli of sequence type ST131 in animals and foods (2011) Vet Microbiol, 153, pp. 99-108 Rogers, B.A., Sidjabat, H.E., Paterson, D.L., Escherichia coli O25b-ST131: A pandemic, multiresistant, community-associated strain (2011) J Antimicrob Chemother, 66, pp. 1-14 Peirano, G., Asensi, M.D., Pitondo-Silva, A., Pitout, J.D., Molecular characteristics of extended-spectrum b-lactamase-producing Escherichia coli from Rio de Janeiro, Brazil (2011) Clin Microbiol Infect, 17, pp. 1039-1043 Berman, H., Barberino, M.G., Moreira, E.D., Riley, L., Reis, J.N., Distribution of strain type and antimicrobial susceptibility of Escherichia coli causing meningitis in a large urban setting in Brazil (2014) Journal of Clinical Microbiology Kawamura, K., Goto, K., Nakane, K., Arakawa, Y., Molecular Epidemiology of Extended-Spectrum β-Lactamases and Escherichia coli Isolated from Retail Foods Including Chicken Meat in Japan (2014) Foodborne Pathog Dis, 11, pp. 104-110 Mora, A., Herrera, A., Mamani, R., López, C., Alonso, M.P., Recent Emergence of Clonal Group O25b:K1:H4-B2-ST131 ibeA Strains among Escherichia coli Poultry Isolates, Including CTX-M-9-Producing Strains, and Comparison with Clinical Human Isolates (2010) Applied and Environmental Microbiology, 76, pp. 6991-6997 Minarini, L.A.R., Camargo, I.L.B.C., Pitondo-Silva, A., Darini, A.L.C., Multilocus sequence typing of uropathogenic ESBL-producing Escherichia coli isolated in a Brazilian community (2007) Current Microbiology, 55 (6), pp. 524-529. , DOI 10.1007/s00284-007-9026-3 Valverde, A., Cantón, R., Garcillán-Barcia, M.P., Novais, Â., Galán, J.C., Spread of blaCTX-M-14 Is Driven Mainly by IncK Plasmids Disseminated among Escherichia coli Phylogroups A, B1, and D in Spain (2009) Antimicrobial Agents and Chemotherapy, 53, pp. 5204-5212