A New Amperometric Biosensor For Salicylate Based On Salicylate Hydroxylase Immobilized On Polipyrrole Film Doped With Hexacyanoferrate

dc.creatorMilagres B.G.
dc.creatorDe Oliveira Neto G.
dc.creatorKubota L.T.
dc.creatorYamanaka H.
dc.date1997
dc.date2015-06-30T14:47:15Z
dc.date2015-11-26T14:34:31Z
dc.date2015-06-30T14:47:15Z
dc.date2015-11-26T14:34:31Z
dc.date.accessioned2018-03-28T21:37:55Z
dc.date.available2018-03-28T21:37:55Z
dc.identifier
dc.identifierAnalytica Chimica Acta. , v. 347, n. 01/02/15, p. 35 - 41, 1997.
dc.identifier32670
dc.identifier10.1016/S0003-2670(97)00105-0
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0030842924&partnerID=40&md5=c379cd91bbeb14f255c6050a41f3375e
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/99966
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/99966
dc.identifier2-s2.0-0030842924
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1248127
dc.descriptionAn amperometric biosensor for salicylate detection was developed by immobilizing salicylate hydroxylase via glutaraldehyde onto a polypyrrole film doped with hexacyanoferrate, supported on a glassy carbon electrode surface. The sensor monitors the catechol produced in the enzymatic reaction on the film surface, at an applied potential of 150 mV vs. SCE. A [NADH]/[salicylate] ratio between 2 and 4 gave the best response. The biosensor presented the best performance in a solution with pH=7.4. The response time was about 40 s. A linear range of response was observed for salicylate concentrations between 1.0x10-5 and 1.0x10-4 mol l-1 and the equation adjusted for this curve was I=(-0.04±0.01)+(11.4±0.2)[salicylate] with a correlation coefficient of 0.999 for n=6. The biosensor retains its activity for at least 10 days despite daily use. The results obtained using the biosensor for salicylate determination, in three different samples of antithermic drugs, presented a good correlation with the standard colorimetric method.
dc.description347
dc.description01/02/15
dc.description35
dc.description41
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dc.languageen
dc.publisher
dc.relationAnalytica Chimica Acta
dc.rightsfechado
dc.sourceScopus
dc.titleA New Amperometric Biosensor For Salicylate Based On Salicylate Hydroxylase Immobilized On Polipyrrole Film Doped With Hexacyanoferrate
dc.typeArtículos de revistas


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