Artículos de revistas
Effectiveness, Against Tuberculosis, Of Pseudo-ternary Complexes: Peptide-dna-cationic Liposome
Registro en:
Journal Of Colloid And Interface Science. , v. 373, n. 1, p. 102 - 109, 2012.
219797
10.1016/j.jcis.2011.09.040
2-s2.0-84858281305
Autor
Rosada R.S.
Silva C.L.
Santana M.H.A.
Nakaie C.R.
De la Torre L.G.
Institución
Resumen
We report the effects of a synthetic peptide designed to act as a nuclear localization signal on the treatment of tuberculosis. The peptide contains 21 amino acid residues with the following specific domains: nuclear localization signal from SV 40T, cationic shuttle sequence, and cysteamide group at the C-terminus. The peptide was complexed with the plasmid DNAhsp65 and incorporated into cationic liposomes, forming a pseudo-ternary complex. The same cationic liposomes, composed of egg chicken l-α-phosphatidylcholine, 1,2-dioleoyl-3-trimethylammonium-propane, and 1,2-dioleoyl-3-trimethylammonium-propane (2:1:1. M), were previously evaluated as a gene carrier for tuberculosis immunization protocols with DNAhsp65. The pseudo-ternary complex presented a controlled size (250. nm), spherical-like shape, and various lamellae in liposomes as evaluated by transmission electron microscopy. 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