Investigation Of The Cytocidal Potential Of Rhinella Jimi Skin Methanol Extracts

dc.creatorBrito S.V.
dc.creatorSales D.L.
dc.creatorCosta J.G.M.
dc.creatorRodrigues F.F.G.
dc.creatorFerreira F.S.
dc.creatorAngelico E.C.
dc.creatorCarvalho J.E.
dc.creatorAlmeida W.O.
dc.creatorAnjos L.A.
dc.creatorCoutinho H.D.M.
dc.date2012
dc.date2015-06-26T20:30:37Z
dc.date2015-11-26T14:32:04Z
dc.date2015-06-26T20:30:37Z
dc.date2015-11-26T14:32:04Z
dc.date.accessioned2018-03-28T21:35:25Z
dc.date.available2018-03-28T21:35:25Z
dc.identifier
dc.identifierPharmaceutical Biology. , v. 50, n. 8, p. 1026 - 1030, 2012.
dc.identifier13880209
dc.identifier10.3109/13880209.2012.655858
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84863702417&partnerID=40&md5=c29cb27b5a5eabbe063913e6164e18f6
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/97401
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/97401
dc.identifier2-s2.0-84863702417
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1247516
dc.descriptionContext: Amphibian skins have wide variety of biologically active compounds associated with the natural defenses of these animals. Objectives: To study the in vitro anticancer activity of methanol extracts of the skin of Rhinella jimi Stevaux (Anura: Bufonidae). Material and methods: The extract was obtained by cold methanol extraction for 96 h using dried skins (295 mg). The methanol skin extract was dried under reduced pressure, giving a 5.5% yield. In order to test for growth-inhibitory activity, in vitro tests were performed with the following cancer cell lines using concentrations ranging between 0.25-250 μg/mL of the extract by 48 h: K562 (leukemia), MCF-7 (breast), NCI-ADR (breast with MDR phenotype), UACC-62 (melanoma), NCI460 (lung), PCO3 (prostate), HT-29 (colon), OVCAR (ovary), and 786-0 (kidney). Results: The methanol extract of R. jimi produced a growth inhibition in a dose-dependent manner against the most of the assayed cell lines. In addition to the growth inhibition, the extract induced the cell death in the ovary and colon lines (EC50 0.125 and 0.2 μg/mL, respectively), demonstrating 100% of inhibition with 2.5 μg/mL. However, prostate and leukemia cell lines demonstrated less sensitivity, with EC50 of 24 and 235 μg/mL, respectively. This is the first report about the anticancer activity by natural products from the skin of R. jimi. Conclusions: The methanol extracts of R. jimi significantly affected the growth of several cell lines, demonstrating that these compounds are a potential source of substances that could be utilized in cancer treatments. © 2012 Informa Healthcare USA, Inc.
dc.description50
dc.description8
dc.description1026
dc.description1030
dc.descriptionAkiyama, M., Ogura, M., Iwai, M., Iijima, M., Numazawa, S., Yoshida, T., Effect of bufalin on growth and differentiation of human skin carcinoma cells in vitro (1999) Hum. Cell, 12, pp. 205-209
dc.descriptionAnjos, L.A., Silva, L.E.M., Almeida, W.O., Costa, J.G.M., Vasconcellos, A., Chaunus jimi (NCN) endoparasites (2008) Herpetolo. Rev., 39, pp. 337-338
dc.descriptionCoutinho, H.D., Lobo, K.M., Bezerra, D.A., Lobo, I., Peptides and proteins with antimicrobial activity (2008) Indian J. Pharmacol, 40, pp. 3-9
dc.descriptionDaly, J.W., Noimai, N., Kongkathip, B., Kongkathip, N., Wilham, J.M., Garraffo, H.M., Kaneko, T., Chan-Ard, T., Biologically active substances from amphibians: Preliminary studies on anurans from twenty-one genera of Thailand (2004) Toxicon, 44, pp. 805-815
dc.descriptionDaly, J.W., Spande, T.F., Garraffo, H.M., Alkaloids from amphibian skin: A tabulation of over eight-hundred compounds (2005) J. Nat. Prod., 68, pp. 1556-1575
dc.descriptionDoyle, J., Brinkworth, C.S., Wegener, K.L., Carver, J.A., Llewellyn, L.E., Olver, I.N., Bowie, J.H., Tyler, M.J., Nnos inhibition, antimicrobial and anticancer activity of the amphibian skin peptide, citropin 1.1 and synthetic modifications The solution structure of a modified citropin 1.1 (2003) Eur. J. Biochem., 270, pp. 1141-1153
dc.descriptionGiri, B., Gomes, A., Debnath, A., Saha, A., Biswas, A.K., Dasgupta, S.C., Gomes, A., Antiproliferative, cytotoxic and apoptogenic activity of Indian toad (Bufo melanostictus, Schneider) skin extract on U937 and K562 cells (2006) Toxicon, 48, pp. 388-400
dc.descriptionGomes, A., Giri, B., Kole, L., Saha, A., Debnath, A., Gomes, A., A crystalline compound (BM-ANF1) from the Indian toad (Bufo melanostictus, Schneider) skin extract, induced antiproliferation and apoptosis in leukemic and hepatoma cell line involving cell cycle proteins (2007) Toxicon, 50, pp. 835-849
dc.descriptionGomes, A., Giri, B., Saha, A., Mishra, R., Dasgupta, S.C., Debnath, A., Gomes, A., Bioactive molecules from amphibian skin: Their biological activities with reference to therapeutic potentials for possible drug development (2007) Indian J. Exp. Biol., 45, pp. 579-593
dc.descriptionHalliday, D.C., Venables, D., Moore, D., Shanmuganathan, T., Pallister, J., Robinson, A.J., Hyatt, A., Cane toad toxicity: An assessment of extracts from early developmental stages and adult tissues using MDCK cell culture (2009) Toxicon, 53, pp. 385-391
dc.descriptionHashimoto, S., Jing, Y., Kawazoe, N., Masuda, Y., Nakajo, S., Yoshida, T., Kuroiwa, Y., Nakaya, K., Bufalin reduces the level of topoisomerase II in human leukemia cells and affects the cytotoxicity of anticancer drugs (1997) Leuk Res., 21, pp. 875-883
dc.descriptionHenriques, A.T., Limberger, R.P., Kerberv, A., Moreno, P.R.H., Alcaloides: Generalidades e aspectos basicos (2007) Farmacognosia da Planta ao Medicamento, pp. 651-666. , Simoes CMO, Schenkel EP, Gosmann G, Mello JCP, Mentz LA, Petrovick PR, eds. Porto Alegre: Ed UFRGS
dc.description(2005) Perfil Basico MunicipalFortaleza: Governo Do Estado Do Ceara - Secretaria Do Planejamento e Coordenacao, , IPECE - Instituto De Pesquisa Estrategica Economica Do Ceara
dc.descriptionJared, C., Antoniazzi, M.M., Jordao, A.E., Silva, J.R., Greven, H., Rodrigues, M.T., Parotoid macroglands in toad (Rhinella jimi): Their structure and functioning in passive defence (2009) Toxicon, 54, pp. 197-207
dc.descriptionKamano, Y., Nogawa, T., Yamashita, A., Hayashi, M., Inoue, M., Drasar, P., Pettit, G.R., Isolation and structure of a 20,21-epoxybufenolide series from Ch'an Su (2002) J. Nat. Prod., 65, pp. 1001-1005
dc.descriptionMaciel, N.M., Schwartz, C.A., Rodrigues Pires Jr., O., Sebben, A., Castro, M.S., Sousa, M.V., Fontes, W., Ferroni Schwartz, E.N., Composition of indolealkylamines of Bufo rubescens cutaneous secretions compared to six other Brazilian bufonids with phylogenetic implications (2003) Comp. Biochem. Physiol. B. Biochem. Mol. Biol., 134, pp. 641-649
dc.descriptionMatos, F.J.A., (1997) Introducao a Fitoquimica Experimental Fortaleza, , ED-UFC
dc.descriptionMonks, A., Scudiero, D., Skehan, P., Shoemaker, R., Paull, K., Vistica, D., Hose, C., Vaigro-Wolff, A., Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines (1991) J. Natl. Cancer Inst., (83), pp. 757-766
dc.descriptionMortari, M.R., Schwartz, E.N., Schwartz, C.A., Pires Jr., O.R., Santos, M.M., Bloch Jr., C., Sebben, A., Main alkaloids from the Brazilian dendrobatidae frog Epipedobates flavopictus: Pumiliotoxin 251D, histrionicotoxin and decahydroquinolines (2004) Toxicon, 43, pp. 303-310
dc.descriptionNasu, K., Nishida, M., Ueda, T., Takai, N., Bing, S., Narahara, H., Miyakawa, I., Bufalin induces apoptosis and the G0/G1 cell cycle arrest of endometriotic stromal cells: A promising agent for the treatment of endometriosis (2005) Mol. Hum. Reprod., 11, pp. 817-823
dc.descriptionSilva, G.R., Santos, C.L., Alves, M.R., Sousa, S.D.V., Annuziata, B.B., Anfibios das dunas litoraneas do extremo norte do Piaui, Brasil (2007) Sitientibus, 7, pp. 334-340
dc.descriptionSkehan, P., Storeng, R., Scudiero, D., Monks, A., McMahon, J., Vistica, D., Warren, J.T., Boyd, M.R., New colorimetric cytotoxicity assay for anticancer-drug screening (1990) J. Natl. Cancer Inst., 82, pp. 1107-1112
dc.descriptionStevaux, M.N., The new species of bufo (anura, bufonidae) in the Northeastern Brazil (2002) Rev. Bras. Zool., 19, pp. 235-242
dc.descriptionTempone, A.G., Pimenta, D.C., Lebrun, I., Sartorelli, P., Taniwaki, N.N., De Andrade Jr., H.F., Antoniazzi, M.M., Jared, C., Antileishmanial and antitrypanosomal activity of bufadienolides isolated from the toad Rhinella jimi parotoid macrogland secretion (2008) Toxicon, 52, pp. 13-21
dc.descriptionYeh, J.Y., Huang, W.J., Kan, S.F., Wang, P.S., Effects of bufalin and cinobufagin on the proliferation of androgen dependent and independent prostate cancer cells (2003) Prostate, 54, pp. 112-124
dc.languageen
dc.publisher
dc.relationPharmaceutical Biology
dc.rightsfechado
dc.sourceScopus
dc.titleInvestigation Of The Cytocidal Potential Of Rhinella Jimi Skin Methanol Extracts
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución