Brasil | Artículos de revistas

The Zn2 Position In Metallo-β-lactamases Is Critical For Activity: A Study On Chimeric Metal Sites On A Conserved Protein Scaffold

dc.creatorGonzalez J.M.
dc.creatorMedrano Martin F.J.
dc.creatorCostello A.L.
dc.creatorTierney D.L.
dc.creatorVila A.J.
dc.date2007
dc.date2015-06-30T18:36:49Z
dc.date2015-11-26T14:30:10Z
dc.date2015-06-30T18:36:49Z
dc.date2015-11-26T14:30:10Z
dc.date.accessioned2018-03-28T21:33:27Z
dc.date.available2018-03-28T21:33:27Z
dc.identifier
dc.identifierJournal Of Molecular Biology. , v. 373, n. 5, p. 1141 - 1156, 2007.
dc.identifier222836
dc.identifier10.1016/j.jmb.2007.08.031
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-35148836146&partnerID=40&md5=f2f64e68012be4b534fdacd6c6eb1063
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/103990
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/103990
dc.identifier2-s2.0-35148836146
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1247032
dc.descriptionMetallo-β-lactamases (MβLs) are bacterial Zn(II)-dependent hydrolases that confer broad-spectrum resistance to β-lactam antibiotics. These enzymes can be subdivided into three subclasses (B1, B2 and B3) that differ in their metal binding sites and their characteristic tertiary structure. To date there are no clinically useful pan-MβL inhibitors available, mainly due to the unawareness of key catalytic features common to all MβL brands. Here we have designed, expressed and characterized two double mutants of BcII, a di-Zn(II) B1-MβL from Bacillus cereus, namely BcII-R121H/C221D (BcII-HD) and BcII-R121H/C221S (BcII-HS). These mutants display modified environments at the so-called Zn2 site or DCH site, reproducing the metal coordination environments of structurally related metallohydrolases. Through a combination of structural and functional studies, we found that BcII-HD is an impaired β-lactamase even as a di-Zn(II) enzyme, whereas BcII-HS exhibits the ability to exist as mono or di-Zn(II) species in solution, with different catalytic performances. We show that these effects result from an altered position of Zn2, which is incapable of providing a productive interaction with the substrate β-lactam ring. These results indicate that the position of Zn2 is essential for a productive substrate binding and hydrolysis. © 2007 Elsevier Ltd. All rights reserved.
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dc.languageen
dc.publisher
dc.relationJournal of Molecular Biology
dc.rightsfechado
dc.sourceScopus
dc.titleThe Zn2 Position In Metallo-β-lactamases Is Critical For Activity: A Study On Chimeric Metal Sites On A Conserved Protein Scaffold
dc.typeArtículos de revistas


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