Effect Of Betamethasone And Diclofenac Sodium On Serum And Tissue Concentration Of Amoxicillin. In Vivo Study In Rats

dc.creatorMattos Filho T.R.
dc.creatorJunqueira M.D.S.
dc.creatorGroppo F.C.
dc.creatorMotta R.H.L.
dc.creatorPerazzo F.F.
dc.date2006
dc.date2015-06-30T18:18:28Z
dc.date2015-11-26T14:29:12Z
dc.date2015-06-30T18:18:28Z
dc.date2015-11-26T14:29:12Z
dc.date.accessioned2018-03-28T21:32:25Z
dc.date.available2018-03-28T21:32:25Z
dc.identifier
dc.identifierJournal Of Applied Oral Science. , v. 14, n. 5, p. 319 - 323, 2006.
dc.identifier16787757
dc.identifier
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-33846464207&partnerID=40&md5=3915cd0603dccc791c7d7989a40a8b9b
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/103910
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/103910
dc.identifier2-s2.0-33846464207
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1246778
dc.descriptionObjective: Antimicrobial agents in combination with anti-inflammatory drugs have been usually prescribed in both Medicine and Dentistry. However, few scientific reports support this clinical practice. The aim of this study was to evaluate the effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin in rats. Methods: Four polyurethane sponges were implanted in the back skin of 48 rats. After seven days the animals were divided into 6 groups (n=8). Group 1: amoxicillin (25 mg/kg); G2: diclofenac sodium (2.5 mg/kg); G3: betamethasone (0.1 mg/kg); G4: diclofenac sodium and amoxicillin; G5: betamethasone and amoxicillin; and G6: 0.9% sodium chloride solution (1.0 mL - control group). All drugs were administered in a single dose. After 90 minutes, the granulomatous tissues of each animal were surgically removed and weighed. Blood was collected from cervical plexus, centrifuged and 10μL of serum was placed on paper discs. In order to estimate amoxicillin concentration, serum and granulomatous tissues were separately submitted to microbiological assay, which used 108cfu/mL of Staphylococcus aureus ATCC 25923 (penicillin-susceptible strain). After incubation (18 hours, 37°C) the inhibition zones were measured and compared to a regression curve. Results: No inhibition zones were observed for groups 2, 3 and 6. Tissue and serum concentrations of both G1 (4.14μ g/g and 2.06μ g/mL, respectively) and G5 (3.87μ g/g and 1.70μ g/mL, respectively) showed statistically significant differences (Kruskal-Wallis, p<0.05) in comparison to G4 (1.45μ g/g and 0.41μ g/mL, respectively). G1 and G5 did not differ significantly (p>0.05). Conclusion: Considering single doses, betamethasone did not interfere with amoxicillin levels but diclofenac sodium reduced both tissue and serum levels of amoxicillin in rats.
dc.description14
dc.description5
dc.description319
dc.description323
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dc.languageen
dc.publisher
dc.relationJournal of Applied Oral Science
dc.rightsaberto
dc.sourceScopus
dc.titleEffect Of Betamethasone And Diclofenac Sodium On Serum And Tissue Concentration Of Amoxicillin. In Vivo Study In Rats
dc.typeArtículos de revistas


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