Advantages And Disadvantages Of C18 And C30 Columns For Hplc Separation Of Carotenoids [vantagens E Desvantagens Das Colunas C18 E C30 Para A Separação De Carotenóides Por Clae]

dc.creatorNunes I.L.
dc.creatorMercadante A.Z.
dc.date2006
dc.date2015-06-30T18:16:53Z
dc.date2015-11-26T14:28:56Z
dc.date2015-06-30T18:16:53Z
dc.date2015-11-26T14:28:56Z
dc.date.accessioned2018-03-28T21:32:09Z
dc.date.available2018-03-28T21:32:09Z
dc.identifier
dc.identifierRevista Brasileira De Ciencias Farmaceuticas/brazilian Journal Of Pharmaceutical Sciences. , v. 42, n. 4, p. 539 - 546, 2006.
dc.identifier15169332
dc.identifier
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-33947616337&partnerID=40&md5=3641bb2b3be15f81d0249f7f23581cca
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/103815
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/103815
dc.identifier2-s2.0-33947616337
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1246719
dc.descriptionSeveral studies have demonstrated a high association between dietary intake or plasma levels of carotenoids and the decrease of risk or the protection against some diseases. Taking this into consideration, as well as the high susceptibility of these compounds to light and heat, leading to the formation of cis isomers with lower biological activity, it is important to develop systems that allow the separation of such compounds in foods. This work evaluated the separation of the geometric isomers of lycopene and of the position isomers, lutein and zeaxanthin, by high performance liquid chromatography (HPLC) using C18 (monomeric, 4 mm, 300 x 3.9 mm) and C30 (polymeric 3 mm, 250 x 4.6 mm) columns and many different mobile phases, with either isocratic or gradient elution. The carotenoids were identified by their spectral characteristics and co-chromatography with standards. The best chromatographic conditions were achieved with the C30 column, temperature set at 33°C and as mobile phase an isocratic elution of methanol (0.1% triethylamine)/tert-butyl methyl ether (50:50) to separate lycopene isomers and (95:5) for lutein and zeaxanthin, both at 1 mL/min. However, for quantitative analysis, it is necessary to evaluate the peak area repeatability on the C 30 column. In addition, the monomeric C18 column can be employed for separation of lutein and zeaxanthin.
dc.description42
dc.description4
dc.description539
dc.description546
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dc.languagept
dc.publisher
dc.relationRevista Brasileira de Ciencias Farmaceuticas/Brazilian Journal of Pharmaceutical Sciences
dc.rightsfechado
dc.sourceScopus
dc.titleAdvantages And Disadvantages Of C18 And C30 Columns For Hplc Separation Of Carotenoids [vantagens E Desvantagens Das Colunas C18 E C30 Para A Separação De Carotenóides Por Clae]
dc.typeArtículos de revistas


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