| dc.creator | de Carvalho R.A. | |
| dc.creator | Torres T.T. | |
| dc.creator | de Azeredo-Espin A.M.L. | |
| dc.date | 2006 | |
| dc.date | 2015-06-30T18:12:51Z | |
| dc.date | 2015-11-26T14:27:27Z | |
| dc.date | 2015-06-30T18:12:51Z | |
| dc.date | 2015-11-26T14:27:27Z | |
| dc.date.accessioned | 2018-03-28T21:30:35Z | |
| dc.date.available | 2018-03-28T21:30:35Z | |
| dc.identifier | | |
| dc.identifier | Veterinary Parasitology. , v. 140, n. 03/04/15, p. 344 - 351, 2006. | |
| dc.identifier | 3044017 | |
| dc.identifier | 10.1016/j.vetpar.2006.04.010 | |
| dc.identifier | http://www.scopus.com/inward/record.url?eid=2-s2.0-33750725686&partnerID=40&md5=aab189994fc7218231df4542218a6dff | |
| dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/103523 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/103523 | |
| dc.identifier | 2-s2.0-33750725686 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1246344 | |
| dc.description | Cochliomyia hominivorax (Calliphoridae) is one of the most important myiasis-causing flies and is responsible for severe economic losses to the livestock industry throughout the Neotropical region. In Brazil, C. hominivorax has been controlled mainly with organophosphate (OP) insecticides, although the inappropriate use of these chemicals can result in the selection of resistant flies. Changes in carboxylesterase activity have been associated with OP insecticides in some arthopodan species. In this work, we isolated and characterized part of the E3 gene in C. hominivorax (ChαE7), which contained the same substitutions responsible for the acquisition of OP hydrolase activity in Lucilia cuprina (Calliphoridae). Digestion of the polymerase chain reaction products with a restriction enzyme that specifically recognized the mutation site unambiguously differentiated wild and mutated esterase alleles. The PCR-RFLP assay therefore provided a fast, reliable DNA-based method for identifying C. hominivorax individuals with a mutation in the esterase gene. Further bioassays to determine the association of this mutation with OP resistance in C. hominivorax should allow the development of more effective strategies for managing this species. © 2006 Elsevier B.V. All rights reserved. | |
| dc.description | 140 | |
| dc.description | 03/04/15 | |
| dc.description | 344 | |
| dc.description | 351 | |
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| dc.description | Campbell, P.M., Newcomb, R.D., Russel, R.J., Oakeshott, J.G., Two different amino acid substitutions in the ali-esterase E3 confer alternative types of organophosphorus insecticide resistance in the sheep blowfly, Lucilia cuprina (1998) Insect Biochem. Mol. Biol., 28, pp. 139-150 | |
| dc.description | Campbell, P.M., Trott, J.T., Claudianos, C., Smyth, K.A., Russel, R.J., Oakeshott, J.G., Biochemistry of esterases associated with organophosphate resistance with comparisons to putative orthologues in other Diptera (1997) Biochem. Genet., 35, pp. 17-40 | |
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| dc.description | Claudianos, C., Russell, R.J., Oakeshott, J.G., The same amino acid substitution in orthologous esterases confers organophosphate resistance on the house fly and a blowfly (1999) Insect Biochem. Mol. Biol., 29, pp. 675-686 | |
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| dc.description | Newcomb, R.D., Gleeson, D.M., Yong, C.G., Russell, R.J., Oakeshott, J.G., Multiple mutations and gene duplications conferring organophosphorus insecticide resistance have been selected at the Rop-1 locus of the sheep blowfly, Lucilia cuprina (2005) J. Mol. Evol., 60, pp. 207-220 | |
| dc.description | Parker, A.G., Campbell, P.M., Spackman, M.E., Russell, R.J., Oakeshott, J.G., Comparison of an esterase associated with organophosphate resistance in Lucilia cuprina with an orthologue not associated with resistance in Drosophila melanogaster (1996) Pestic. Biochem. Physiol., 55, pp. 85-99 | |
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| dc.description | (1965) Myiasis in Man and Animals in the Old World, , Zumpt F. (Ed), London, Butterworths 267 pp | |
| dc.language | en | |
| dc.publisher | | |
| dc.relation | Veterinary Parasitology | |
| dc.rights | fechado | |
| dc.source | Scopus | |
| dc.title | A Survey Of Mutations In The Cochliomyia Hominivorax (diptera: Calliphoridae) Esterase E3 Gene Associated With Organophosphate Resistance And The Molecular Identification Of Mutant Alleles | |
| dc.type | Artículos de revistas | |
