One and two step extraction processes were used to obtain extracts from two samples of alecrim do campo leaves (Baccharis dracunculifolia L.). In the one step process, supercritical carbon dioxide (scCO 2), ethanol and water were used as solvents. In the two step process, scCO 2 was used as a solvent in the first step followed by ethanol or water as the solvent in a second step on the residue resulting from scCO 2 extraction. Extraction yields, total polyphenol, total flavonoid and Artepillin C contents, antioxidant activity by DPPH radical scavenging and β-carotene bleaching methods were measured in all extracts. The supercritical extract showed a higher concentration and yield of Artepillin C, low antioxidant activity by the DPPH method and high antioxidant activity by the β-carotene method. In general the aqueous and ethanol extracts were more concentrated in polyphenol compounds and a second extraction step produced high yields with high concentrations of phenolic compounds. © 2011 Elsevier B.V. All rights reserved.63 3139Frizzo, C.D., Atti-Serafini, L., Laguna, S.E., Cassel, E., Lorenzo, D., Dellacassa, E., Essential oil variability in Baccharis uncinella DC and Baccharis dracunculifolia DC growing wild in southern Brazil, Bolivia and Uruguay (2008) Flavour and Fragrance Journal, 23 (2), pp. 99-106. , DOI 10.1002/ffj.1862Lago, J.H.G., Romoff, P., Fávero, O.A., Soares, M.G., Baraldi, P.T., Correa, A.G., Souza, F.O., Composition of essential oils from the leaves of six species of the Baccharis genus from "campos de altitude" of the Atlantic forest of São Paulo (2008) Quimica Nova, 31, pp. 727-730Da Silva Filho, A.A., De Sousa, J.P.B., Soares, S., Furtado, N.A.J.C., Andrade E Silva, M.L., Cunha, W.R., Gregorio, L.E., Bastos, J.K., Antimicrobial activity of the extract and isolated compounds from Baccharis dracunculifolia D. C. (Asteraceae) (2008) Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 63 (1-2), pp. 40-46Cassel, E., Frizzo, C.D., Vanderlinde, R., Atti-Serafini, L., Lorenzo, D., Dellacassa, E., Extraction of Baccharis oil by supercritical CO 2 (2000) Industrial & Engineering Chemistry Research, 39, pp. 4803-4805Weyerstahl, P., Christiansen, C., Marschall, H., Constituents of Brazilian vassoura oil (1996) Flavour and Fragrance Journal, 11 (1), pp. 15-23Alencar, S.M., Aguiar, C.L., Paredes-Guzman, J., Park, Y.K., Chemical composition of Baccharis dracunculifolia, the botanical source of propolis from the states of São Paulo and Minas Gerais (2005) Ciencia Rural, 35, pp. 909-915Park, Y.K., Paredes-Guzman, J.F., Aguiar, C.L., Alencar, S.M., Fujiwara, F.Y., Chemical constituents in Baccharis dracunculifolia as the main botanical origin of southeastern Brazilian Propolis (2004) J. Agricultural Food Chemistry, 52, pp. 1100-1103Funari, C.S., Ferro, V.O., Propolis analysis (2006) Ciência e Tecnologia de Alimentos, 26, pp. 171-178Teixeira, E.W., Message, D., Negri, G., Salatino, A., Stringheta, P.C., Seasonal variation, chemical composition and antioxidant activity of Brazilian propolis samples (2010) Evidence Based Complementary and Alternative Medicine, 7, pp. 307-315Paulino, N., Abreu, S.R.L., Uto, Y., Koyama, D., Nagasawa, H., Hori, H., Dirsch, V.M., Bretz, W.A., Anti-inflammatory effects of a bioavailable compound, Artepillin C, in Brazilian propolis (2008) European J. Pharmacology, 587, pp. 296-301Kimoto, T., Aga, M., Hino, K., Koya-Miyata, S., Yamamoto, Y., Micallef, M.J., Hanaya, T., Kurimoto, M., Apoptosis of human leukemia cells induced by Artepillin C, an active ingredient of Brazilian propolis (2001) Anticancer Research, 21 (1 A), pp. 221-228Kimoto, T., Koya-Miyata, S., Hino, K., Micallef, M.J., Hanaya, T., Arai, S., Ikeda, M., Kurimoto, M., Pulmonary carcinogenesis induced by ferric nitrilotriacetate in mice and protection from it by Brazilian propolis and artepillin C (2001) Virchows Archiv, 438 (3), pp. 259-270. , DOI 10.1007/s004280000350Shimizu, K., Das, S.K., Hashimoto, T., Sowa, Y., Yoshida, T., Sakai, T., Matsuura, Y., Kanazawa, K., Artepillin C in Brazilian propolis induces G 0/G 1 arrest via stimulation of Cip1/p21 expression in human colon cancer cells (2005) Molecular Carcinogenesis, 44 (4), pp. 293-299. , DOI 10.1002/mc.20148Piantino, C.R., Aquino, F.W.B., Follegatti-Romero, L.A., Cabral, F.A., Supercritical extraction of phenolic compounds from Baccharis dracunculifolia (2008) J. Supercritical Fluids, 47, pp. 209-214Mukhopadhyay, M., (2000) Natural Extracts Using Supercritical Carbon Dioxide, pp. 225-248. , CRC Press Boca RatonMuchuweti, M., Mupure, C., Ndhlala, A., Murenje, T., Benhura, M.A.N., Screening of antioxidant and radical scavenging activity of Vigna ungiculata, Bidens pilosa and Cleome gynandra (2007) American Journal of Food Technology, 2 (3), pp. 161-168. , http://www.academicjournals.net/fulltext/ajft/2007/161-168.pdfMartinez-Correa, H.A., Magalhães, P.M., Queiroga, C.L., Peixoto, C.A., Oliveira, A.L., Cabral, F.A., Extracts from pitanga (Eugenia uniflora L.) leaves: Influence of extraction process on antioxidant properties and yield of phenolic compounds (2011) J. Supercritical Fluids, 55 (3), pp. 998-1006(1997) Method of Determining and Expressing Fineness of Feed Materials by Sieving, p. 547. , A.S.A.E. Standards, ASAE S319.3Uquiche, E., Del Valle, J.M., Ortiz, J., Supercritical carbon dioxide extraction of red pepper (Capsicum annuum L.) oleoresin (2004) J. Food Engineering, 65, pp. 55-66Rahman, M.S., Perera, C.O., Chen, X.D., Driscoll, R.H., Potluri, P.L., Density, shrinkage and porosity of calamari mantle meat during air drying in a cabinet dryer as a function of water content (1996) Journal of Food Engineering, 30 (1-2), pp. 135-145. , PII S0260877496000131Cseke, L.J., Setzer, W.N., Vogler, B., Kirakosyan, A., Kaufman, P.B., Traditional, analytical and preparative separations of natural products (2006) Natural Products from Plants, pp. 263-318. , L.J. Cseke, A. Kirakosyan, P.B. Kaufman, S.L. Warber, J.A. Duke, H.L. Brielman, 2nd ed. CRC Press Boca RatonSingleton, V.L., Orthofer, R., Lamuela-Raventos, R.M., Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent (1998) Methods in Enzymology, 299, pp. 152-178. , DOI 10.1016/S0076-6879(99)99017-1Zhishen, J., Mengcheng, T., Jianming, W., The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals (1999) Food Chemistry, 64 (4), pp. 555-559. , DOI 10.1016/S0308-8146(98)00102-2, PII S0308814698001022Adams, R.P., (1995) Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, p. 469. , Allured Publishing CorporationMensor, L.L., Menezes, F.S., Leitão, G.G., Reis, A.S., Dos Santos, T.C., Coube, C.S., Leitão, S.G., Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method (2001) Phytotherapy Research, 15, pp. 127-130Marco, J.G., A rapid determination for evaluation of antioxidants (1968) J. the American Oil Chemists' Society, 45, pp. 594-598Miller, H.E., A simplified method for the evaluation of antioxidant (1971) J. the American Oil Chemists Society, 48, p. 91Grigonis, D., Venskutonis, P.R., Sivik, B., Sandahl, M., Eskilsson, C.S., Comparison of different extraction techniques for isolation of antioxidants from sweet grass (Hierochloe odorata) (2005) Journal of Supercritical Fluids, 33 (3), pp. 223-233. , DOI 10.1016/j.supflu.2004.08.006, PII S0896844604001482Massignani, J.J., Lemos, M., Maistro, E.L., Schaphauser, H.P., Jorge, R.F., Sousa, J.P.B., Bastos, J.K., Andrade, S.F., Antiulcerogenic activity of the essential oil of Baccharis dracunculifolia on different experimental models in rats (2009) Phytotherapy Research, 23, pp. 1355-1360Queiroga, C.L., Bastos, J.K., De Sousa, J.P.B., De Magalhaes, P.M., Comparison of the chemical composition of the essential oil and the water soluble oil of Baccharis dracunculifolia DC. (Asteraceae) (2008) Journal of Essential Oil Research, 20 (2), pp. 111-114Gobbo-Neto, L., Lopes, N.P., Plantas medicinais: Fatores de influência no conteúdo de metabólitos secundários (2007) Química Nova, 32, pp. 374-381Lopes, S.C.P., Blanco, Y.C., Justo, G.Z., Nogueira, P.A., Rodrigues, F.L.S., Goelnitz, U., Wunderlich, G., Costa, F.T.M., Violacein extracted from chromobacterium violaceum inhibits plasmodium growth in vitro and in vivo (2009) Antimicrobial Agents and Chemotherapy, 53, pp. 2149-2152Loayza, I., Abujder, D., Aranda, R., Jakupovic, J., Collin, G., Deslauriers, H., Jean, F., Essential oils of Baccharis salicifolia, Baccharis latifolia and Baccharis dracunculifolia (1995) Phytochemistry, 38, pp. 381-389Schossler, P., Schneider, G.L., Wunsch, D., Soares, G.L.G., Zini, C.A., Volatile compounds of baccharis punctulata, baccharis dracunculifolia and eupatorium laevigatum obtained using solid phase microextraction and hydrodistillation (2009) J. the Brazilian Chemical Society, 20, pp. 277-287Moon, J.K., Shibamoto, T., Antioxidant assays for plant and food components (2009) J. Agricultural and Food Chemistry, 57, pp. 1655-1666Czyewski, E., Barbisan, E.M., Rosa, R.D.L., Cansian, R.L., Emmerich, D.J., Loch, C., Paroul, N., Atividade Antioxidante dos Extratos Etanólicos de Carqueja (Baccharistrimera) e Vassoura (Baccharis dracunculifolia) (2008) Proceedings of XVI Encontro de Química da Região sul, p. 273. , G. Machado, Blumenau, BrazilFrankel, E.N., Huang, S.W., Kanner, J., German, J.B., Interfacial phenomena in the evaluation of antioxidants: Bulk oils vs. emulsions (1994) J. Agricultural and Food Chemistry, 42, pp. 1054-1059Koleva, I.I., Niederlander, H.A.G., Van Beek, T.A., An on-line HPLC method for detection of radical scavenging compounds in complex mixtures (2000) Analytical Chemistry, 72 (10), pp. 2323-2328. , DOI 10.1021/ac9912451Pouvreau, J.B., Moranais, M., Taran, F., Rosa, P., Dufossé, L., Guérard, F., Pin, S., Pondaven, P., Antioxidant and free radical scavenging properties of marennine, a blue-green polyphenolic pigment from the diatom Haslea ostrearia (Gaillon/Bory) Simonsen responsible for the natural greening of cultured oysters (2008) J. Agricultural and Food Chemistry, 56, pp. 6278-6286