Artículos de revistas
Unraveling The Variability And Genetic Structure Of Barker Frog Physalaemus Cuvieri (leiuperinae) Populations From Different Regions Of Brazil
Registro en:
Genetics And Molecular Research. Fundacao De Pesquisas Cientificas De Ribeirao Preto, v. 13, n. 3, p. 8055 - 8065, 2014.
16765680
10.4238/2014.February.21.4
2-s2.0-84907843034
Autor
Conte M.
Targueta C.P.
Zucchi M.I.
Souza A.P.
Recco-Pimentel S.M.
Institución
Resumen
The barker frog Physalaemus cuvieri is widely distributed in South America and is found in all regions of Brazil. Significant intraspecific morphological variation in this species has been reported. To determine the genetic structure of the natural Brazilian populations of P. cuvieri, 10 different populations geographically separated by 99.41 to 2936.75 km were evaluated using 10 polymorphic microsatellite loci. In addition, mitochondrial DNA data were analyzed to determine genetic distance between the populations. The genetic variation was found to be significant in most of the populations (HE ranged from 0.40 to 0.59, and allelic richness ranged from 2.07 to 3.54). An FST value of 0.27 indicated that high genetic structure was present among the P. cuvieri populations. STRUCTURE. 13 3 8055 8065 Barber, P.H., Patterns of gene flow and population genetic structure in the canyon treefrog, Hyla arenicolor (Cope) (1999) Mol. Ecol, 8, pp. 563-576 Barreto, L., Andrade, G.V., Aspects of the reproductive biology of Physalaemus cuvieri (Anura: Leptodactylidae) in northeastern Brazil (1995) Amphibia-Reptilia, 16, pp. 67-76 Barrio, A., El gênero Physalaemus (Anura, Leptodactylidae) en la Argentina (1965) Physis, 25, pp. 421-448 Berven, K.A., Grudzien, T.A., Dispersal in the Wood Frog (Rana sylvatica): Implications for genetic population structure (1990) Evolution, 44, pp. 2047-2056 Burns, E.L., Eldridge, M.D., Houlden, B.A., Microsatellite variation and population structure in a declining Australian Hylid Litoria aurea (2004) Mol. Ecol, 13, pp. 1745-1757 Conte, M., Cançado, L.J., Laborda, P.R., Zucchi, M.I., Isolation and characterization of polymorphic microsatellites for the natural populations of barker frog Physalaemus cuvieri (2009) Conserv. Genet, 10, pp. 1849-1852 Conte, M., Zucchi, M.I., Andrade, G.V., Souza, A.P., Study of closely related species within the Physalaemus cuvieri group (Anura): Contribution of microsatellite markers (2011) Genet. Mol. Res, 10, pp. 1434-1444 Creste, S., Tulmann, N.A., Figueira, A., Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining (2001) Plant Mol. Biol. Rep, 19, pp. 299-306 Driscoll, D.A., Genetic structure, metapopulation processes and evolution influence the conservation strategies for two endangered frog species (1998) Biol. Conserv, 83, pp. 43-54 Driscoll, D.A., Genetic structure of the frog Geocrinia lutea and Geocrinia rosea reflects extreme population divergence and range changes, not dispersal barriers (1998) Evolution, 52, pp. 1147-1157 Ersts, P.J., Geographic Distance Matrix Generator (version 1.2.3) American Museum of Natural History (2007) Center for Biodiversity and Conservation, (2011). , http://biodiversityinformatics.amnh.org/open_source/gdmg, Available at, Accessed October 8 Evanno, G., Regnaut, S., Goudet, J., Detecting the number of clusters of individuals using the software Structure: A simulation study (2005) Mol. Ecol, 14, pp. 2611-2620 Ficetola, G.F., De Bernardi, F., Amphibians in a human-dominated landscape: The community structure is related to habitat features and isolation (2004) Biol. Conserv, 119, pp. 219-230 Fouquet, A., Gilles, A., Vences, M., Marty, C., Underestimation of species richness in Neotropical frogs revealed by mtDNA analyses (2007) PLoS One, 2 Fouquet, A., Vences, M., Salducci, M.D., Meyer, A., Revealing cryptic diversity using molecular phylogenetics and phylogeography in frogs of the Scinax ruber and Rhinella margaritifera species groups (2007) Mol. Phylogenet. Evol, 43, pp. 567-582 Frost, D.R., (2013) Amphibians Species of the World: An Online, , http://research.amnh.org/vz/herpetology/amphibia/, Reference. Version 5.6 (January 9, 2011). Electronic Database Available at , American Museum of Natural History, New York Frost, D.R., Grant, T., Faivovich, J., Bain, R., The Amphibian Tree of Life (2006) Bulletin American Museum of Natural History, (297). , Research Library, New York Funk, W.C., Blouin, M.S., Corn, P.S., Maxell, B.A., Population structure of Columbia spotted frogs (Rana luteiventris) is strongly affected by the landscape (2005) Mol. Ecol, 14, pp. 483-496 Funk, W.C., Cannatella, D.C., Ryan, M.J., Genetic divergence is more tightly related to call variation than landscape features in the Amazonian frogs Physalaemus petersi and P. freibergi (2009) J. Evol. Biol, 22, pp. 1839-1853 (2009), http://www.globalamphibians.org, (2009). 2009 IUCN/Species Survival Commission/CI/CABS/Nature Service. Available at, Accessed May 10Goebel, A.M., Donnelly, J.M., Atz, M.E., PCR primers and amplification methods for 12S ribosomal DNA, the control region, cytochrome oxidase I, and cytochrome b in bufonids and other frogs, and an overview of PCR primers which have amplified DNA in amphibians successfully (1999) Mol. Phylogenet. Evol, 11, pp. 163-199 Goudet, J., FSTAT (VERSION 1.2): A Computer Program To Calculate F-Statistics (1995) J. Hered, 86, pp. 485-486 Johansson, M., Primmer, C.R., Merila, J., Does habitat fragmentation reduce fitness and adaptability? A case study of the common frog (Rana temporaria) (2007) Mol. Ecol, 16, pp. 2693-2700 Kraaijeveld-Smit, F.J., Beebee, T.J., Griffiths, R.A., Moore, R.D., Low gene flow but high genetic diversity in the threatened Mallorcan midwife toad Alytes muletensis (2005) Mol.Ecol, 14, pp. 3307-3315 Lewis, P.O., Zaykin, D., (2000) Genetic data analysis: Computer program for the analysis of allelic data, , http://alleyn.eeb.uconn.edu/gda/2000, Version 1.0 (d15). Free program distributed by authors over the Internet from the GDA. Available at Manier, M.K., Arnold, S.J., Ecological correlates of population genetic structure: A comparative approach using a vertebrate metacommunity (2006) Proc. Biol. Sci, 273, pp. 3001-3009 Martins, V.G., Bacci, M., Jr., (2001) Métodos Moleculares Para o Estudo do DNA I - Extração e Amplificação de DNA: Protocolos, , Centro de Estudos de Insetos Sociais, UNESP/Rio Claro Nascimento, L.B., Caramaschi, U., Cruz, C.A.G., Taxonomic review of the species groups of the genus Physalaemus Fitzinger, 1826 with revalidation of the genera Engystomops Jiménez-de-La-Espada, 1872 and Eupemphix Steindachner, 1863 (Amphibia, Anura, Leptodactylidae) (2005) Arq. Mus. Nac. Rio de Janeiro, 63, pp. 297-320 Newman, R.A., Squire, T., Microsatellite variation and fine-scale population structure in the wood frog (Rana sylvatica) (2001) Mol. Ecol, 10, pp. 1087-1100 Pritchard, J.K., Stephens, M., Donnelly, P., Inference of population structure using multilocus genotype data (2000) Genetics, 155, pp. 945-959 Pyron, R.A., Wiens, J.J., A large-scale phylogeny of Amphibia including over 2800 species, and a revised classification of extant frogs, salamanders, and caecilians (2011) Mol. Phylogenet. Evol, 61, pp. 543-583 Quinderé, Y.R.S.D., Tomatis, C., Baldo, D., Lourenço, L.B., Polytypic and polymorphic cytogenetic variations in the widespread anuran Physalaemus cuvieri (Anura, Leiuperidae) with emphasis on nucleolar organizing regions (2009) Biol. Res, 42, pp. 79-92 Robertson, J.M., Duryea, M.C., Zamudio, K.R., Discordant patterns of evolutionary differentiation in two Neotropical treefrogs (2009) Mol. Ecol, 18, pp. 1375-1395 Ron, S.R., Santos, J.C., Cannatella, D.C., Phylogeny of the tungara frog genus Engystomops (= Physalaemus pustulosus species group Anura: Leptodactylidae). Mol. Phylogenet (2006) Evol, 39, pp. 392-403 Rowe, G., Beebee, T.J.C., Burke, T., A microsatellite analysis of natterjack toad, Bufo calamita, metapopulations (2000) Oikos, 88, pp. 641-651 Schmeller, D.S., Merilä, J., Demographic and genetic estimates of effective population and breeding size in the amphibian Rana temporaria (2007) Conserv. Biol, 21, pp. 142-151 Seppä, P., Laurila, A., Genetic structure of island populations of the anurans Rana temporaria and Bufo bufo (1999) Heredity, 82, pp. 309-317 Shaffer, H.B., Fellers, G.M., Magee, A., Voss, S.R., The genetics of amphibian declines: Population substructure and molecular differentiation in the yosemite toad, Bufo canorus (Anura, Bufonidae) based on single-strand conformation polymorphism analysis (SSCP) and mitochondrial DNA sequence data (2000) Mol. Ecol, 9, pp. 245-257 Silva, A.P.Z., Haddad, C.F.B., Kasahara, S., Nucleolus organizer regions in Physalaemus cuvieri (Anura, Leptodactylidae), with evidence of a unique case of Ag-NOR variability (1999) Hereditas, 131, pp. 135-141 Tamura, K., Dudley, J., Nei, M., Kumar, S., MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 (2007) Mol. Biol. Evol, 24, pp. 1596-1599 Telles, M.P., Bastos, R.P., Soares, T.N., Resende, L.V., RAPD variation and population genetic structure of Physalaemus cuvieri (Anura: Leptodactylidae) in Central Brazil (2006) Genetica, 128, pp. 323-332 Veith, M., Molecular markers and species delimitation: Examples from the European batrachofauna (1996) Amphibia- Reptilia, 7, pp. 303-314 Vences, M., Thomas, M., Van Der Meijden, A., Chiari, Y., Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians (2005) Front. Zool, 2, p. 5 Wright, S., The genetical structure of populations (1951) Ann. Eugenics, 15, pp. 321-354