Hpv Detection Using Primers My09/my11 And Gp5+/gp6+ In Patients With Cytologic And/or Colposcopic Changes

dc.creatorVenceslau E.M.
dc.creatorBezerra M.M.
dc.creatorLopes A.C.M.
dc.creatorSouza E.V.
dc.creatorOnofre A.S.C.
dc.creatorDe Melo C.M.
dc.creatorDe Lourdes Sierpe Jeraldo V.
dc.creatorDe Miranda Onofre F.B.
dc.date2014
dc.date2015-06-25T17:53:48Z
dc.date2015-11-26T14:26:42Z
dc.date2015-06-25T17:53:48Z
dc.date2015-11-26T14:26:42Z
dc.date.accessioned2018-03-28T21:29:45Z
dc.date.available2018-03-28T21:29:45Z
dc.identifier
dc.identifierJornal Brasileiro De Patologia E Medicina Laboratorial. Sociedade Brasileira De Pneumologia E Tisiologia, v. 50, n. 4, p. 280 - 285, 2014.
dc.identifier16762444
dc.identifier10.5935/1676-2444.20140028
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84908550777&partnerID=40&md5=e1e143411d2d865c55606a2b84d68daf
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/86531
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/86531
dc.identifier2-s2.0-84908550777
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1246141
dc.descriptionIntroduction: Cervical cancer is one of the most common diseases among women, and cause considerable morbidity and mortality. Considering that cervical cancer is an important neoplasia in northeastern Brazil, and the prevalence of high-risk human papillomavirus (HPV) is directly associated with it, this work had aimed to correlate the cytological and/or colposcopic findings with HPV infection status, and verify the performance of MY09/MY11 and GP5+/6+ primers for HPV detection. Material and method: Patients in this study were from Penedo-AL, a city with high level of poverty (poverty rate of 60.62%). Out of 70 patients with cytological and/or colposcopic changes, 32 agreed to participate in the study. Results: Regarding cytology, 21 (30%) patients presented atypical squamous cells of undetermined significance (ASC-US); 20 (29%), atypical glandular cells of undetermined significance (AGUS); 12 (17%), low-grade intraepithelial lesion (LSIL); five (7%), high-grade intraepithelial lesion (HSIL); and 12 (17%), positive colposcopy. From these, 27 (84%) presented the band gene encoding for human ß-globin. From the 27 patients, eight (30%) were positive for HPV. The results showed that the deoxyribonucleic acid (DNA) of HPV was detected in 15% and 30% by using MY-PCR and GP +-PCR, respectively. Conclusion: This study suggests that more than one type of oligonucleotide primer should be used in clinical samples to increase sensitivity for the detection of HPV.
dc.description50
dc.description4
dc.description280
dc.description285
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dc.languageen
dc.publisherSociedade Brasileira de Pneumologia e Tisiologia
dc.relationJornal Brasileiro de Patologia e Medicina Laboratorial
dc.rightsaberto
dc.sourceScopus
dc.titleHpv Detection Using Primers My09/my11 And Gp5+/gp6+ In Patients With Cytologic And/or Colposcopic Changes
dc.typeArtículos de revistas


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