Avian Extraintestinal Escherichia Coli Exhibits Enterotoxigenic-like Activity In The In Vivo Rabbit Ligated Ileal Loop Assay

dc.creatorMaluta R.P.
dc.creatorGatti M.S.V.
dc.creatorJoazeiro P.P.
dc.creatorDe Paiva J.B.
dc.creatorRojas T.C.G.
dc.creatorSilveira F.
dc.creatorHoule S.
dc.creatorKobayashi R.K.T.
dc.creatorDozois C.M.
dc.creatorDias Da Silveira W.
dc.date2014
dc.date2015-06-25T17:53:08Z
dc.date2015-11-26T14:21:23Z
dc.date2015-06-25T17:53:08Z
dc.date2015-11-26T14:21:23Z
dc.date.accessioned2018-03-28T21:23:09Z
dc.date.available2018-03-28T21:23:09Z
dc.identifier
dc.identifierFoodborne Pathogens And Disease. Mary Ann Liebert Inc., v. 11, n. 6, p. 484 - 489, 2014.
dc.identifier15353141
dc.identifier10.1089/fpd.2013.1719
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84902013652&partnerID=40&md5=2625e4d420fa51c0948b626e584dbb52
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/86393
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/86393
dc.identifier2-s2.0-84902013652
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1244519
dc.descriptionAvian pathogenic Escherichia coli (APEC) strains harbor a number of virulence genes and cause extraintestinal diseases, such as septicemia, swollen-head syndrome, salpingitis, and omphalitis in poultry. APEC strains are not known to cause intestinal diseases. Herein, for the first time, it is reported that APEC strains were able to induce an enterotoxigenic-like effect in rabbit ligated ileal loops. Strain SEPT362 caused cell detachment of the intestinal villi, which also showed a flattened and wilted appearance, but the integrity of the tight junctions was maintained. Additionally, this strain did not adhere to enterocytes in vivo, although adhesin encoding genes (fimH, csgA, lpfA2-3, and ECP) were present while other lpfA types, sfa, afa, papC, and ral genes were not. This enterotoxigenic-like activity was conserved after thermal treatment of the supernatant at 65°C but not at 100°C. Moreover, experiments based on filtering with different molecular weight cut-off (MWCO) pore sizes demonstrated that the component associated with the observed biological effect has a molecular weight >100 kDa. Blast search and polymerase chain reaction assays for known E. coli virulence factors showed that strain SEPT362 harbors the gene encoding for the toxin EAST-1 and the serine protease autotransporter (SPATE) Tsh, but is negative for genes encoding for the toxins LT-I, STh, STp, Stx1, Stx2, CNF-1, CNF-2, CDT and the SPATEs Sat, Pic, Vat, SigA, SepA, EatA, EspP, or EspC. A cloned copy of the tsh gene in E. coli K-12 was also tested and was shown to have an enterotoxic effect. These results suggest that APEC might induce fluid accumulation in the rabbit gut. The Tsh autotransporter seems to be one of the factors associated with this phenotype. © Copyright 2014, Mary Ann Liebert, Inc. 2014.
dc.description11
dc.description6
dc.description484
dc.description489
dc.descriptionAdlerberth, I., Hanson, L., Svanborg, C., Svennerholm, A., Nordgren, S., Wold, A., Adhesins of Escherichia coli associated with extra-intestinal pathogenicity confer binding to colonic epithelial cells (1995) Microb Pathog, 18, pp. 373-385
dc.descriptionBauchart, P., Germon, P., Brée, A., Oswald, E., Hacker, J., Dobrindt, U., Pathogenomic comparison of human extraintestinal and avian pathogenic Escherichia coli-Search for factors involved in host specificity or zoonotic potential (2010) Microb Pa-thog, 49, pp. 105-115
dc.descriptionBélanger, L., Garenaux, A., Harel, J., Boulianne, M., Nadeau, E., Dozois, C.M., Escherichia coli from animal reservoirs as a potential source of human extraintestinal pathogenic E coli (2011) FEMS Immunol Med Microbiol, 62, pp. 1-10
dc.descriptionBlanco, J.E., Blanco, M., Mora, A., Blanco, J., Production of toxins (enterotoxins, verotoxins, and necrotoxins) and colicins by Escherichia coli strains isolated from septicemic and healthy chickens: Relationship with in vivo pathogenicity (1997) J Clin Microbiol, 35, pp. 2953-2957
dc.descriptionBorges, C.A., Beraldo, L.G., Maluta, R.P., Shiga toxigenic and atypical enteropathogenic Escherichia coli in the feces and carcasses of slaughtered pigs (2012) Foodborne Pathog Dis, 9, pp. 1119-1125
dc.descriptionDe Pace, F., Boldrin De Paiva, J., Nakazato, G., Characterization of IcmF of the type VI secretion system in an avian pathogenic Escherichia coli (APEC) strain (2011) Microbiology, 157, pp. 2954-2962
dc.descriptionDziva, F., Stevens, M., Colibacillosis in poultry: Unravelling the molecular basis of virulence of avian pathogenic Escherichia coli in their natural hosts (2008) Avian Pathol, 37, pp. 355-366
dc.descriptionMatsuda, K., Chaudhari, A.A., Lee, J.H., Avian colibacillosis caused by an intestinal pathogenic Escherichia coli isolate from calf diarrhea (2010) Res Vet Sci, 89, pp. 150-152
dc.descriptionMellata, M., Human and avian extraintestinal pathogenic Es-cherichia coli: Infections, zoonotic risks, and antibiotic resistance trends (2013) Foodborne Pathog Dis, 10, pp. 916-932
dc.descriptionMénard, L.-P., Lussier, J.G., Lépine, F., De Sousa, C.P., Dubreuil, J.D., Expression, purification, and biochemical characterization of enteroaggregative Escherichia coli heat-stable enterotoxin 1 (2004) Protein Expr Purif, 33, pp. 223-231
dc.descriptionMoulin-Schouleur, M., Répérant, M., Laurent, S., Extra-intestinal pathogenic Escherichia coli strains of avian and human origin: Link between phylogenetic relationships and common virulence patterns (2007) J Clin Microbiol, 45, pp. 3366-3376
dc.descriptionNavarro-Garcia, F., Gutierrez-Jimenez, J., Garcia-Tovar, C., Castro, L.A., Salazar-Gonzalez, H., Cordova, V., Pic, an autotransporter protein secreted by different pathogens in the En-terobacteriaceae family, is a potent mucus secretagogue (2010) Infect Immun, 78, pp. 4101-4109
dc.descriptionOlesen, B., Scheutz, F., Andersen, R.L., Enteroaggregative Escherichia coli O78:H10 the cause of an outbreak of urinary tract infection (2012) J Clin Microbiol, 50, pp. 3703-3711
dc.descriptionProvence, D.L., Curtiss, R., Isolation and characterization of a gene involved in hemagglutination by an avian pathogenic Es-cherichia coli strain (1994) Infect Immun, 62, pp. 1369-1380
dc.descriptionTcg, R., Maluta, R.P., Parizzi, L.P., Genome sequences of avian pathogenic Escherichia coli strains isolated from Brazilian commercial poultry (2013) Genome Announce, 1, pp. e00110-e00113
dc.descriptionRusso, T.A., Johnson, J.R., Proposal for a new inclusive designation for extraintestinal pathogenic isolates of Escherichia coli: ExPEC (2000) J Infect Dis, 181, pp. 1753-1754
dc.descriptionSavarino, S.J., Fasano, A., Robertson, D.C., Levine, M.M., En-teroaggregative Escherichia coli elaborate a heat-stable en-terotoxin demonstrable in an in vitro rabbit intestinal model (1991) J Clin Invest, 87, pp. 1450-1455
dc.descriptionSchierack, P., Walk, N., Ewers, C., ExPEC-typical virulence-associated genes correlate with successful colonization by intestinal E coli in a small piglet group (2008) Environ Microbiol, 10, pp. 1742-1751
dc.descriptionSyngkon, A., Elluri, S., Koley, H., Studies on a novel serine protease of a DhapADprtV Vibrio cholerae O1 strain and its role in hemorrhagic response in the rabbit ileal loop model (2010) PLoS ONE, 5. , pii:e13122
dc.descriptionMam, V., Tat, G., Ajp, F., Knöbl, T., Servin, A.L., Liévin-Le Moal, V., Two atypical enteropathogenic Escher-ichia coli strains induce the production of secreted and membrane-bound mucins to benefit their own growth at the apical surface of human mucin-secreting intestinal HT29-MTX cells (2010) Infect Immun, 78, pp. 927-938
dc.languageen
dc.publisherMary Ann Liebert Inc.
dc.relationFoodborne Pathogens and Disease
dc.rightsfechado
dc.sourceScopus
dc.titleAvian Extraintestinal Escherichia Coli Exhibits Enterotoxigenic-like Activity In The In Vivo Rabbit Ligated Ileal Loop Assay
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución