Inhibition Of Carrageenin-induced Rat Paw Oedema By Crotapotin, A Polypeptide Complexed With Phospholipase A2

dc.creatorLanducci E.C.T.
dc.creatorAntunes E.
dc.creatorDonate J.L.
dc.creatorFare R.
dc.creatorHyslop S.
dc.creatorMarangoni S.
dc.creatorOliveira B.
dc.creatorCirino G.
dc.creatorDe Nucci G.
dc.date1995
dc.date2015-06-26T17:14:49Z
dc.date2015-11-26T14:20:48Z
dc.date2015-06-26T17:14:49Z
dc.date2015-11-26T14:20:48Z
dc.date.accessioned2018-03-28T21:22:28Z
dc.date.available2018-03-28T21:22:28Z
dc.identifier
dc.identifierBritish Journal Of Pharmacology. , v. 114, n. 3, p. 578 - 583, 1995.
dc.identifier71188
dc.identifier
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0028928851&partnerID=40&md5=40ec6263993b65b464169780a25d193a
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/96008
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/96008
dc.identifier2-s2.0-0028928851
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1244345
dc.descriptionThe effect of purified crotapotin, a non-toxic non-enzymatic chaperon protein normally complexed to a phospholipase A2 (PLA2) in South America rattlesnake venom, was studied in the acute inflammatory response induced by carrageenin (1 mg/paw), compound 48/80 (3 μg/paw) and 5-hydroxytryptamine (5-HT) (3 μg/paw) in the rat hind-paw. The effects of crotapotin on platelet aggregation, mast cell degranulation and eicosanoid release from guinea-pig isolated lung were also investigated. Subplantar co-injection of crotapotin (1 and 10 μg/paw) with carrageenin or injection of crotapotin (10 μg/paw) into the contralateral paw significantly inhibited the carrageenin-induced oedema. This inhibition was also observed when crotapotin (10-30 μg/paw) was administered either intraperitoneally or orally. Subplantar injection of heated crotapotin (15 min at 60°C) failed to inhibit carrageenin-induced oedema. Subplantar injection of crotapotin (10 μg/paw) also significantly inhibited the rat paw oedema induced by compound 48/80, but it did not affect 5-HT-induced oedema. In adrenalectomized animals, subplantar injection of crotapotin markedly inhibited the oedema induced by carrageenin. The inhibitory effect of crotapotin was also observed in rats depleted of histamine and 5-HT stores. Crotapotin (30 μg/paw) had no effect on either the histamine release induced by compound 48/80 in vitro or on the platelet aggregation induced by both arachidonic acid (1 mM) and platelet activating factor (1 μM) in human platelet-rich plasma. The platelet aggregation and thromboxane B2 (TXB2) release induced by thrombin (100 mu ml-1) in washed human platelets were also not affected by crotapotin. In addition, crotapotin (10 μg/paw) did not affect the release of 6-oxo-prostaglandin F(1α) and TXB2 induced by ovalbumin in sensitized guinea-pig isolated lungs. Our results indicate that the anti-inflammatory activity of crotapotin is not due to endogenous corticosteroid release or inhibition of cyclo-oxygenase activity. It is possible that crotapotin may interact with extracellular PLA2 generated during the inflammatory process thereby reducing its hydrolytic activity.
dc.description114
dc.description3
dc.description578
dc.description583
dc.languageen
dc.publisher
dc.relationBritish Journal of Pharmacology
dc.rightsfechado
dc.sourceScopus
dc.titleInhibition Of Carrageenin-induced Rat Paw Oedema By Crotapotin, A Polypeptide Complexed With Phospholipase A2
dc.typeArtículos de revistas


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