Changes In Peroxidases In The Suspension Culture Of Rubus Fruticosus During Growth

dc.creatorCortelazzo A.L.
dc.creatorMarais M.-F.
dc.creatorJoseleau J.-P.
dc.date1996
dc.date2015-06-26T17:02:41Z
dc.date2015-11-26T14:18:26Z
dc.date2015-06-26T17:02:41Z
dc.date2015-11-26T14:18:26Z
dc.date.accessioned2018-03-28T21:19:42Z
dc.date.available2018-03-28T21:19:42Z
dc.identifier
dc.identifierPlant Cell, Tissue And Organ Culture. , v. 46, n. 1, p. 27 - 33, 1996.
dc.identifier1676857
dc.identifier
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0030477770&partnerID=40&md5=b23cc4fbabe9cc4211968e84ddb26483
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/95470
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/95470
dc.identifier2-s2.0-0030477770
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1243637
dc.descriptionThe growth parameters of a cell suspension culture of Rubus fruticosus L. were determined over a culture period including exponential growth, stationary phase and a glucose starvation period at the end of the normal culture cycle. Peroxidase activities were measured in the cytoplasm, in the cell wall, and in the culture medium by the guaiacol assay. There is a relationship between the activity found in the spent medium and the dry matter mass of the cells during the exponential growth. In the three compartments a bimodal repartition of peroxidase activities was observed, with the two peaks at day 4 and day 26, respectively. This suggests that the first peak corresponds to actively dividing cells whereas the second is associated with senescence, or stress due to starvation. Fractionation of the peroxidases from the culture medium revealed the presence of two sets of cationic isoenzymes, with minor amount of anionic peroxidases. Interestingly, the second peak of cationic enzymes which was of weak intensity at day 10 of the culture, became prevalent at day 26. This indicates that not only the total amount of peroxidases varies as a function of culture time, but also that the nature of the peroxidases secreted into the medium changes during growth.
dc.description46
dc.description1
dc.description27
dc.description33
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dc.languageen
dc.publisher
dc.relationPlant Cell, Tissue and Organ Culture
dc.rightsfechado
dc.sourceScopus
dc.titleChanges In Peroxidases In The Suspension Culture Of Rubus Fruticosus During Growth
dc.typeArtículos de revistas


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