Paracoccidioides Brasiliensis-gp43 Used As Paracoccidioidin

dc.creatorSaraiva E.C.O.
dc.creatorAltemani A.
dc.creatorFranco M.F.
dc.creatorUnterkircher C.S.
dc.creatorCamargo Z.P.
dc.date1996
dc.date2015-06-26T17:02:52Z
dc.date2015-11-26T14:18:21Z
dc.date2015-06-26T17:02:52Z
dc.date2015-11-26T14:18:21Z
dc.date.accessioned2018-03-28T21:19:38Z
dc.date.available2018-03-28T21:19:38Z
dc.identifier
dc.identifierJournal Of Medical And Veterinary Mycology. , v. 34, n. 3, p. 155 - 161, 1996.
dc.identifier2681218
dc.identifier
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0029661850&partnerID=40&md5=6b30913d4970edf5fb9f0f6a30cb1e03
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/95486
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/95486
dc.identifier2-s2.0-0029661850
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1243617
dc.descriptionA purified glycoprotein of 43 000 daltons from Paracoccidioides brasiliensis (gp43) was tested as paracoccidioidin in delayed-type hypersensitivity (DTH) tests in both experimental animals (guinea pig and mice) and patients with paracoccidioidomycosis (PCM). The gp43 paracoccidioidin was compared with the traditional Fava Netto antigen (AgFN). In guinea pigs, the intradermal injection of 2 μg of gp43 showed a similar response to those obtained with AgFN, showing in histological sections a population of lymphoid cells that participate in DTH. In mice, gp43 at a dose of 3.75μg showed positive DTH response. The use of gp43 as paracoccidioidin in humans showed that this molecule can be used to evaluate the DTH response in patients with PCM. Of 25 PCM patients studied, 48% were positive to gp43 while only 28% were positive to AgFN; 12 PCM patients were completely anergic to both antigens. Considering only those 13 PCM patients who were responsive to gp43 and/or to AgFN, 92.3% reacted against gp43 and 53.8% reacted against AgFN (P < 0.05). Gp43 skin test responses (13.67 ± 9.56 mm) were significantly larger than those obtained with AgFN (8.43 ±3.69 mm). Immunohistochemical study of the human skin showed a perivascular inflammatory response constituted predominantly by T lymphocytes, macrophages and polymorphonuclear leukocytes. © 1996 ISHAM.
dc.description34
dc.description3
dc.description155
dc.description161
dc.descriptionFranco, M.F., Host-parasite relationships in paracoccidioidomycosis (1987) J Med Vet Mycol, 25, pp. 5-18
dc.descriptionMota, N.G.S., Rezkallah-Iwasso, M.T., Peraçoli, M.T.S., Correlation between cell-mediated immunity and clinical forms of paracoccidioidomycosis (1985) Trans Royal Soc Trop Med Hyg, 79, pp. 765-772
dc.descriptionDe Almeida, F.P., Da Lacaz, C.S., Da Cunha, Ç.A., Intradermoreação para diagnostico da blastomicose Sul-Americana (granulomatose-paracoccidióidica) (1945) Arq Bras Med, 351, pp. 267-272
dc.descriptionDouat, N.E., Diaz, V.M., Intradermoreações de paracoccidioidina, coccidioidina e histoplasmina: Resultados de testes em 300 individuos (1953) Rev Bras Tuberculose, 26, pp. 663-668
dc.descriptionMackinnon, J.E., Artagaveytia-Allende, R.C., Arroyo, L., Sobre la especificidad de la intradermorreacción con paracoccidioidina (1953) An Fac Med Montevideo, 38, pp. 363-382
dc.descriptionFava Netto, C., Raphael, A., A reação intradérmica corn polissacáride do Paracoccidioides brasiliensis, na blastomicose Sul-Americana (1961) Rev Inst Med Trop São Paulo, 3, pp. 161-165
dc.descriptionRestrepo-Marena, A., Schneidau, J.D., Nature of the skin-reactive principle in culture filtrates prepared from Paracoccidioides brasiliensis (1967) J Bacterial, 93, pp. 1741-1748
dc.descriptionLacaz, C.S., Porto, E., Martins, J.E.C., Paracoccidioidomicose (1984) Micologia Médica. Fungos, Actinomicetos e Algas de Interesse Médico, 7th Edn., pp. 189-216. , São Paulo: Sarvier
dc.descriptionPuccia, R., Schenckman, S., Gorin, P.A., Travassos, L.R., Exocellular components of Paracoccidioides brasiliensis: Identification of a specific antigen (1986) Infect Immun, 53, pp. 199-206
dc.descriptionCamargo, Z.P., Taborda, C.P., Rodrigues, E.G., Travassos, L.R., The use of cell-free antigens of Paracoccidioides brasiliensis in serological tests (1991) J Med Vet Mycol, 29, pp. 31-38
dc.descriptionCamargo, Z., Unterkircher, C., Campoy, S.P., Travassos, L.R., Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests (1988) J Clin Microbiol, 26, pp. 2147-2151
dc.descriptionBlotta, M.H.S., Camargo, Z.P., Immunological response to cell-free antigens of Paracoccidioides brasiliensis: Relationship wih clinical forms of paracoccidioidomycosis (1993) J Clin Microbol, 31, pp. 671-676
dc.descriptionCamargo, Z.P., Unterkircher, C., Travassos, L.R., Identification of antigenic polypeptides of Paracoccidioides brasiliensis by immunoblotting (1989) J Med Vet Mycol, 27, pp. 407-412
dc.descriptionBradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochem, 72, pp. 248-254
dc.descriptionFava Netto, C., Contribuição para o estudo imunológico da blastomicose de Lutz (1961) Rev Inst Adolfo Lutz, 21, pp. 99-194
dc.descriptionHsu, S.M., Raine, L., Fanger, H., The use of antiavidin antibody and avidin-biotin-peroxidase complex in immunoperoxidase techniques (1981) Am J Clin Pathol, 75, pp. 816-821
dc.descriptionLenzini, L., Rottoli, P., Rottoli, L., The spectrum of human tuberculosis (1977) Clin Exp Immunol, 27, pp. 230-237
dc.descriptionMoscardi, M., De Franco, M.F., Paracoccidioidomicose expérimental do camundongo. I. Aspectos immunopatológicos da infecçào intraperitoneal (1980) Rev Inst Med Trop São Paulo, 22, pp. 286-293
dc.descriptionMarchal, G., Milon, G., Numeration of DTH mediating T lymphocytes in mice under optimal titration conditions (1984) Ann Immunol, 135 C, pp. 353-364
dc.descriptionMarchai, G., Seman, M., Milon, G., Truffa-Bachi, P., Zilberfarb, V., Local adoptive tranfer of skin delayed-type hypersensitivity initiated by a single T lymphocyte (1982) J Immunol, 129, pp. 954-958
dc.descriptionBarker, S.A., Cruickshank, C.N.D., Morris, J.H., Wood, S.R., The isolation of trichophytin glycopeptide and its structure in relation to the immediate and delayed reactions (1962) Immunology, 5, pp. 627-632
dc.languageen
dc.publisher
dc.relationJournal of Medical and Veterinary Mycology
dc.rightsfechado
dc.sourceScopus
dc.titleParacoccidioides Brasiliensis-gp43 Used As Paracoccidioidin
dc.typeArtículos de revistas


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