Selection Of Stabilizing Additive For Lipase Immobilization On Controlled Pore Silica By Factorial Design

dc.creatorSoares C.M.F.
dc.creatorDe Castro H.F.
dc.creatorSantana M.H.
dc.creatorZanin G.M.
dc.date2001
dc.date2015-06-26T14:43:13Z
dc.date2015-11-26T14:16:29Z
dc.date2015-06-26T14:43:13Z
dc.date2015-11-26T14:16:29Z
dc.date.accessioned2018-03-28T21:17:28Z
dc.date.available2018-03-28T21:17:28Z
dc.identifier
dc.identifierApplied Biochemistry And Biotechnology - Part A Enzyme Engineering And Biotechnology. , v. 91-93, n. , p. 703 - 718, 2001.
dc.identifier2732289
dc.identifier10.1385/ABAB:91-93:1-9:703
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0035010788&partnerID=40&md5=3326d4eb97ce059982c3e19fd55aeaa3
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/94997
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/94997
dc.identifier2-s2.0-0035010788
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1243072
dc.descriptionCandida rugosa lipase was covalently immobilized on silanized controlled pore silica (CPS) previously activated with glutaraldehyde in the presence of several additives to improve the performance of the immobilized form in long-term operation. Proteins (albumin and lecithin) and organic molecules (β-cyclodextrin and polyethylene glycol [PEG]-1500) were added during the immobilization procedure, and their effects are reported and compared to the behavior of the immobilized biocatalyst in the absence (lacking) of additive. The selection of the most efficient additive at different lipase loadings (150-450 U/g of dry support) was performed by experimental design. Two 22 full factorial designs with two repetitions at the center point were employed to evaluate the immobilization yield. A better stabilizing effect was found when small amounts of albumin or PEG-1500 were added simultaneously to the lipase onto the support. The catalytic activity had a maximum (193 U/mg) for lipase loading of 150 U/g of dry support using PEG-1500 as the stabilizing additive. This immobilized system was used to perform esterification reactions under repeated batch cycles (for the synthesis of butyl butyrate as a model). The half-life of the lipase immobilized on CPS in the presence of PEG-1500 was found to increase fivefold compared with the control (immobilized lipase on CPS without additive).
dc.description91-93
dc.description
dc.description703
dc.description718
dc.descriptionFaber, K., (1997) Biotransformation in Organic Chemistry: A Textbook, 3rd Ed., , Springer-Verlag, Berlin
dc.descriptionYahya, A.R.M., Anderson, W.A., Moo-Young, M., (1998) Enzyme Microb. Technol., 23, pp. 438-450
dc.descriptionReslow, M., Adlercreutz, P., Mattiason, B., (1988) Eur. J. Biochem., 172, pp. 573-578
dc.descriptionBalcão, V.M., Paiva, A.L., Malcata, F.X., (1996) Enzyme Microb. Technol., 18, pp. 392-416
dc.descriptionBosley, J.A., Peilow, A.D., (1997) J. Am. Oil Chem. Soc., 74, pp. 107-111
dc.descriptionSoares, C.M.F., De Castro, H.F., De Moraes, F.F., Zanin, G.M., (1999) Appl. Biochem. Biotechnol., 77-79, pp. 745-758
dc.descriptionReetz, M.T., Zonta, A., Simpelkamp, J., (1996) Biotechnol. Bioeng., 49, pp. 527-534
dc.descriptionWehtje, E., Adlercreutz, P., Mattiasson, B., (1993) Biotechnol. Bioeng., 41, pp. 171-178
dc.descriptionRocha, J.M.S., Gil, M.H., Garcia, F.A.P., (1998) J. Biotechnol., 66, pp. 61-67
dc.descriptionTriantafyllou, A.O., Wehtje, E., Adlercreutz, P., Mattiasson, B., (1995) Biotechnol. Bioeng., 45, pp. 406-414
dc.descriptionTriantafyllou, A.O., Wehtje, E., Adlercreutz, P., Mattiasson, B., (1997) Biotechnol. Bioeng., 54, pp. 67-76
dc.descriptionBox, G.E.P., Hunter, W.G., Hunter, J.S., (1978) Statistics for Experimenters: An Introduction to Design, Data Analysis and Model Building, , Wiley & Sons, New York
dc.descriptionSzejtli, J., (1988) Cyclodextrin Technology, , Kluwer Academic, Boston
dc.descriptionBradford, M.M.A., (1976) Anal. Biochem., 72, pp. 248-254
dc.descriptionDe Castro, H.F., Anderson, W.A., Legge, R.L., Moo-Young, M., (1992) Indian J. Chem., 31, pp. 891-895
dc.languageen
dc.publisher
dc.relationApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
dc.rightsfechado
dc.sourceScopus
dc.titleSelection Of Stabilizing Additive For Lipase Immobilization On Controlled Pore Silica By Factorial Design
dc.typeActas de congresos


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