Modelling And Simulation Of The Adsorption Of The Lipase From Geotrichum Sp. On Hydrophobic Interaction Columns

dc.creatorMendieta-Taboada O.
dc.creatorKamimura E.S.
dc.creatorMaugeri F.
dc.date2001
dc.date2015-06-26T14:42:54Z
dc.date2015-11-26T14:15:53Z
dc.date2015-06-26T14:42:54Z
dc.date2015-11-26T14:15:53Z
dc.date.accessioned2018-03-28T21:16:50Z
dc.date.available2018-03-28T21:16:50Z
dc.identifier
dc.identifierBiotechnology Letters. , v. 23, n. 10, p. 781 - 786, 2001.
dc.identifier1415492
dc.identifier10.1023/A:1010302416897
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0034979584&partnerID=40&md5=85c4bec522aa94c20b534d291531a116
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/94941
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/94941
dc.identifier2-s2.0-0034979584
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1242918
dc.descriptionThe Langmuir model fitted well the adsorption isotherms of lipase on the hydrophobic resin. The model parameters, Qm and kd, were affected by NaCl concentration: Qm increased from 31 to 80 U g-1 resin, and kd changed from 9.4 to 3 U ml-1. Column modelling and the simulation data were compared with the experimental data with good agreement. The highest achieved column efficiency was 71%. Notations: C: lipase concentration in solution (U ml-1); C*: lipase concentration in solution at equilibrium (U ml-1); Co: lipase concentration in the feed (U ml-1); d: resin particle diameter (cm); D: enzyme molecular diffusivity (cm2 min-1); E: axial dispersion coefficient (cm2 min-1); K: global mass transfer coefficient (cm2 min-1); kd: kinetic constant (U ml-1); k1: adsorption kinetic constant (ml U-1 min-1); k2: desorption kinetic constant (min-1); L: bed height (cm); Q: amount of adsorbed lipase in the resin (U ml-1 resin); Q*: amount of adsorbed lipase in the resin at equilibrium (U ml-1 resin); Qm: maximum adsorption capacity of the resin (U ml-1 resin); R: column radius (cm); Rs: rate of lipase adsorption (U ml-1 resin min-1); υ: superficial velocity (cm min-1); t: time; Z: column length (cm); ε: bed porosity. Subscript: i: time, j: position.
dc.description23
dc.description10
dc.description781
dc.description786
dc.descriptionBaillargeon, M.W., McCarthy, S.G., Geotrichum candidum NRRL Y-533 lipase: Purification, characterization and fatty acid specificity (1991) Lipids, 26, pp. 831-836
dc.descriptionBalcão, V.M., Vieira, M.C., Maleata, F.X., Adsorption of protein from several commercial lipase preparations onto hollow-fiber membrane module (1986) Biotechnol. Progr., 12, pp. 164-172
dc.descriptionChase, H.A., Prediction of the performance of preparative affinity chromatography (1984) J. Chromatogr., 297, pp. 179-202
dc.descriptionDiogo, M.M., Silva, S., Cabral, J.M.S., Queiroz, J., A hydrophobic interaction on chromatography of Chromobacterium viscosum lipase on polypropylene glycol immobilised on Sepharose (1999) J. Chromatogr., 849, pp. 413-419
dc.descriptionGitlesen, T., Bauer, M., Adlercreutz, P., Adsorption of lipase on polypropylene powder (1997) Biochim. Biophys. Acta, 1345, pp. 188-196
dc.descriptionGustavsson, P.-E., Axelsson, A., Larsson, P.-O., Superporous agarose on bead as hydrophobic interaction chromatography support (1999) J. Chromatogr., 830, pp. 275-284
dc.descriptionJacobsen, T., Olsen, J., Allerman, K., Substrate specifities of Geotrichum candidum lipase preparations (1990) Biotechnol. Lett., 12, pp. 121-126
dc.descriptionKamimura, E.S., Mendieta, O., Sato, H.H., Pastore, G.M., Maugeri, F., Production of lipase from Geotrichum sp. and adsorption studies on affinity resin (1999) Brazilian J. Chem. Eng., 16, pp. 102-112
dc.descriptionMacêdo, G.A., Park, Y.K., Pastore, G.M., Partial purification and characterization of an extracellular lipase from a newly isolated strain of Geotrichum sp. (1997) Rev. Microbiol., 28, pp. 90-95
dc.descriptionNoriega, J., Tejada, A., Magaña, I., Ortega, J., Guzman, R., Modelling column regeneration effects on dye-ligand affinity chromatography (1997) Biotechnol. Prog., 13, pp. 296-300
dc.descriptionPhillips, A., Pretorious, H.J., Purification and characterization of an extracellular lipase of Galactomyces and Geotrichum (1991) Biotechnol. Lett., 13, pp. 833-838
dc.descriptionPorath, J., Salt promoted adsorption: Recent developments (1986) J. Chromatogr., 376, pp. 331-341
dc.descriptionWarwel, S., Borgdorf, R., Substrate selectivity of lipases in the esterification of cis-trans-isomers and positional isomers of conjugated linoleic acid (CLA) (2000) Biotechnol. Lett., 22, pp. 1151-1155
dc.languageen
dc.publisher
dc.relationBiotechnology Letters
dc.rightsfechado
dc.sourceScopus
dc.titleModelling And Simulation Of The Adsorption Of The Lipase From Geotrichum Sp. On Hydrophobic Interaction Columns
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución