Brasil
| Artículos de revistas
Expression Of Deletion Mutants Of The Hepatitis B Virus Protein Hbx In E. Coli And Characterization Of Their Rna Binding Activities
dc.creator | Rui E. | |
dc.creator | De Moura P.R. | |
dc.creator | Kobarg J. | |
dc.date | 2001 | |
dc.date | 2015-06-26T14:43:04Z | |
dc.date | 2015-11-26T14:15:51Z | |
dc.date | 2015-06-26T14:43:04Z | |
dc.date | 2015-11-26T14:15:51Z | |
dc.date.accessioned | 2018-03-28T21:16:48Z | |
dc.date.available | 2018-03-28T21:16:48Z | |
dc.identifier | ||
dc.identifier | Virus Research. , v. 74, n. 1-2, p. 59 - 73, 2001. | |
dc.identifier | 1681702 | |
dc.identifier | 10.1016/S0168-1702(00)00245-8 | |
dc.identifier | http://www.scopus.com/inward/record.url?eid=2-s2.0-0035114391&partnerID=40&md5=488573b9e9680a08528be3e23dc3a0da | |
dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/94967 | |
dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/94967 | |
dc.identifier | 2-s2.0-0035114391 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1242906 | |
dc.description | The hepatitis B virus protein HBx has been implicated in the development of liver cancer. It has been shown that the HBx protein is able to bind to single-stranded DNA in a specific manner. This DNA binding activity might be relevant for HBx oncogene character. To study the HBx interaction with nucleic acids in more detail we expressed full-length HBx as well as several N- and C-terminally truncated HBx proteins as 6xHis and GST-fusions in E. coli. Using a gel shift assay, we were able to demonstrate that all of the truncated HBx proteins have the ability to bind to an AU-rich RNA. The affinity of GST-HBx #3 (residues 80-142) was an order of magnitude higher than that of GST-HBx #2 (residues 5-79), indicating that a high affinity RNA binding site is located in HBx C-terminal half. AUF1 is the protein ligand that binds to AU-rich RNA regions present in certain proto-oncogene mRNAs and causes their rapid degradation. By a competitive binding experiment of AUF1 and HBx to the AU-rich RNA oligonucleotide, we show that HBx is able to displace AUF1 from its binding site on the RNA oligonucleotide. This new aspect of HBx function is discussed in the context of cellular transformation. © 2001 Elsevier Science B.V. | |
dc.description | 74 | |
dc.description | 1-2 | |
dc.description | 59 | |
dc.description | 73 | |
dc.description | Brewer, G., An A+U-rich element RNA-binding factor regulates c-myc mRNA stability in vitro (1991) Mol.Cell Biol., 11, pp. 2460-2466 | |
dc.description | Carey, J., Gel retardation (1991) Meth. Enzymol., 208, pp. 103-116 | |
dc.description | Challberg, M.D., Kelly, T.J., Animal virus DNA replication (1989) Annu. Rev. Biochem., 58, pp. 671-717 | |
dc.description | Chirillo, P., Pagano, S., Natoli, G., Puri, P.L., Burgio, V.L., Balsano, C., Levrero, M., The hepatitis B virus X gene induces p53-mediated programmed cell death (1997) Proc. Natl. Acad. Sci. USA, 94, pp. 8162-8167 | |
dc.description | Cleveland, D.W., Yen, T.J., Multiple determinants of eukaryotic mRNA stability (1989) New Biol., 1, pp. 121-126 | |
dc.description | Dyda, F., Hickman, A.B., Jenkins, T.M., Engelman, A., Craigie, R., Davies, D.R., Crystal structure of the catalytic domain of HIV-1 integrase: Similarity to other polynucleotidyl transferases (1994) Science, 266, pp. 1981-1986 | |
dc.description | Galibert, F., Mandart, E., Fitoussi, F., Tiollais, P., Charnay, P., Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E.coli (1979) Nature, 281, pp. 646-650 | |
dc.description | Ganem, D., Hepadnaviridae and their replication (1996), pp. 2703-2737. , Fields, B.N., Knipe, D.M., Howley, P.M. (Eds), Fields Virology, 3rd Ed., Lippincott-Raven Publishers, Philadelphia, PAGottlob, K., Pagano, S., Levrero, M., Graessmann, A., Hepatitis B virus X protein transcription activation domains are neither required nor sufficient for cell transformation (1998) Cancer Res., 58, pp. 3566-3570 | |
dc.description | Gupta, A., Mal, T.K., Jayasuryan, N., Chauhan, V.S., Assignment of disulphide bonds in the X protein (HBx) of hepatitis B virus (1995) Biochem. Biophys. Res. Commun., 212, pp. 919-924 | |
dc.description | Hang, X., Dong, W., Guarino, L.A., The lef-3 gene of Autographa californica nuclear polyhedrosis virus encodes a single-stranded DNA-binding protein (1995) J. Virol., 69, pp. 3924-3928 | |
dc.description | Haviv, I., Vaizel, D., Shaul, Y., The X protein of hepatitis B virus coactivates potent activation domains (1995) Mol. Cell Biol., 15, pp. 1079-1085 | |
dc.description | Hohne, M., Schaefer, S., Seifer, M., Feitelson, M.A., Paul, D., Gerlich, W.H., Malignant transformation of immortalized transgenic hepatocytes after transfection with hepatitis B virus DNA (1990) EMBO J., 9, pp. 1137-1145 | |
dc.description | Hollinger, F.B., Hepatitis B virus (1996), pp. 2739-2807. , Fields, B.N., Knipe, D.M. and Howley, P.M. (Eds), Fields Virology, 3rd Ed., Lippincott-Raven Publishers, Philadelphia, PAJameel, S., Siddiqui, A., Maguire, H.F., Rao, K.V., Hepatitis B virus X protein produced in Escherichia coli is biologically functional (1990) J. Virol., 64, pp. 3963-3966 | |
dc.description | Jones, T.R., Cole, M.D., Rapid cytoplasmic turnover of c-myc mRNA: Requirement of the 3′ untranslated sequences (1987) Mol. Cell Biol., 7, pp. 4513-4521 | |
dc.description | Kim, C.M., Koike, K., Saito, I., Miyamura, T., Jay, G., HBx gene of hepatitis B virus induces liver cancer in transgenic mice (1991) Nature, 351, pp. 317-320 | |
dc.description | Klein, N.P., Bouchard, M.J., Wang, L.H., Kobarg, C., Schneider, R.J., Src kinases involved in hepatitis B virus replication (1999) EMBO J., 18, pp. 5019-5027 | |
dc.description | Klimka, A., Barth, S., Drillich, S., Wels, W., Van Snick, J., Renauld, J.C., Tesch, H., Engert, A., A deletion mutant of Pseudomonas exotoxin-A fused to recombinant human interleukin-9 (rhIL-9-ETA′) shows specific cytotoxicity against IL-9- receptor-expressing cell lines (1996) Cytokines Mol. Ther., 2, pp. 139-146 | |
dc.description | Koshland, D., Botstein, D., Secretion of β-lactamase requires the carboxy end of the protein (1980) Cell, 20, pp. 749-760 | |
dc.description | Kumar, V., Jayasuryan, N., Kumar, R., A truncated mutant (residues 58-140) of the hepatitis B virus X protein retains transactivation function (1996) Proc. Natl. Acad. Sci. USA, 93, pp. 5647-5652 | |
dc.description | Kwee, L., Lucito, R., Aufiero, B., Schneider, R.J., Alternate translation initiation on hepatitis B virus X mRNA produces multiple polypeptides that differentially transactivate class II and III promoters (1992) J. Virol., 66, pp. 4382-4389 | |
dc.description | Kwong, P.D., Wyatt, R., Robinson, J., Sweet, R.W., Sodroski, J., Hendrickson, W.A., Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody (1998) Nature, 393, pp. 648-659 | |
dc.description | Lee, W.M., Lin, C., Curran, T., Activation of the transforming potential of the human fos proto-oncogene requires message stabilization and results in increased amounts of partially modified fos protein (1988) Mol. Cell Biol., 8, pp. 5521-5527 | |
dc.description | Ljungquist, C., Breitholtz, A., Brink-Nilsson, H., Moks, T., Uhlen, M., Nilsson, B., Immobilization and affinity purification of recombinant proteins using histidine peptide fusions (1989) Eur. J. Biochem., 186, pp. 563-569 | |
dc.description | Lo, S.J., Chien, M.L., Lee, Y.H., Characteristics of the X gene of hepatitis B virus (1988) Virology, 167, pp. 289-292 | |
dc.description | Maguire, H.F., Hoeffler, J.P., Siddiqui, A., HBVX protein alters the DNA binding specificity of CREB and ATF-2 by protein-protein interactions (1991) Science, 252, pp. 842-844 | |
dc.description | Marczinovits, I., Somogyi, C., Patthy, A., Nemeth, P., Molnar, J., An alternative purification protocol for producing hepatitis B virus X antigen on a preparative scale in Escherichia coli (1997) J. Biotechnol., 56, pp. 81-88 | |
dc.description | Porath, J., Carlsson, J., Olsson, I., Belfrage, G., Metal chelate affinity chromatography, a new approach to protein fractionation (1975) Nature, 258, pp. 598-599 | |
dc.description | Qadri, I., Ferrari, M.E., Siddiqui, A., The Hepatitis B virus transactivator protein, HBx, interacts with single-stranded DNA 9 (ssDNA). Biochemical characterizations of the HBx-ssDNA interactions (1996) J. Biol. Chem., 271, pp. 15443-15450 | |
dc.description | Redeker, A.G., Viral hepatitis: Clinical aspects (1975) Am. J. Med. Sci., 270, pp. 9-16 | |
dc.description | Robinson, W.S., Molecular events in the pathogenesis of hepadnavirus-associated hepatocellular carcinoma (1994) Annu. Rev. Med., 45, pp. 297-323 | |
dc.description | Su, F., Schneider, R.J., Hepatitis B virus HBx protein sensitizes cells to apoptotic killing by tumor necrosis factor α (1997) Proc. Natl. Acad. Sci. USA, 94, pp. 8744-8749 | |
dc.description | Twu, J.S., Wu, J.Y., Robinson, W.S., Transcriptional activation of the human immunodeficiency virus type 1 long terminal repeat by hepatitis B virus X-protein requires de novo protein synthesis (1990) Virology, 177, pp. 406-410 | |
dc.description | Wels, W., Beerli, R., Hellmann, P., Schmidt, M., Marte, B.M., Kornilova, E.S., Hekele, A., Hynes, N.E., EGF receptor and p185erbB-2-specific single-chain antibody toxins differ in their cell-killing activity on tumor cells expressing both receptor proteins (1995) Int. J. Cancer, 60, pp. 137-144 | |
dc.description | Wentz, M.J., Becker, S.A., Slagle, B.L., Dissociation of DDB1-binding and transactivation properties of the hepatitis B virus X protein (2000) Virus Res., 68, pp. 87-92 | |
dc.description | Yasukawa, T., Kanei-Ishii, C., Maekawa, T., Fujimoto, J., Yamamoto, T., Ishii, S., Increase of solubility of foreign proteins in Escherichia coli by coproduction of the bacterial thioredoxin (1995) J. Biol. Chem., 270, pp. 25328-25331 | |
dc.description | Yen, T.S.B., Hepadnaviral X protein: Review of recent progress (1996) J. Biomed. Sci., 3, pp. 20-30 | |
dc.description | Zhang, W., Wagner, B.J., Ehrenman, K., Schaefer, A.W., DeMaria, C.T., Crater, D., DeHaven, K., Brewer, G., Purification, characterization, and cDNA cloning of an AU-rich element RNA-binding protein, AUF1 (1993) Mol. Cell Biol., 13, pp. 7652-7665 | |
dc.language | en | |
dc.publisher | ||
dc.relation | Virus Research | |
dc.rights | fechado | |
dc.source | Scopus | |
dc.title | Expression Of Deletion Mutants Of The Hepatitis B Virus Protein Hbx In E. Coli And Characterization Of Their Rna Binding Activities | |
dc.type | Artículos de revistas |