Decreased Insulin Secretion In Islets From Rats Fed A Low Protein Diet Is Associated With A Reduced Pkaα Expression

dc.creatorFerreira F.
dc.creatorBarbosa H.C.L.
dc.creatorStoppiglia L.F.
dc.creatorDelghingaro-Augusto V.
dc.creatorPereira E.A.
dc.creatorBoschero A.C.
dc.creatorCarneiro E.M.
dc.date2004
dc.date2015-06-26T14:25:39Z
dc.date2015-11-26T14:15:12Z
dc.date2015-06-26T14:25:39Z
dc.date2015-11-26T14:15:12Z
dc.date.accessioned2018-03-28T21:16:05Z
dc.date.available2018-03-28T21:16:05Z
dc.identifier
dc.identifierJournal Of Nutrition. , v. 134, n. 1, p. 63 - 67, 2004.
dc.identifier223166
dc.identifier
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0347986915&partnerID=40&md5=3a53d9836a29fa6cd854de5fffc12269
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/94815
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/94815
dc.identifier2-s2.0-0347986915
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1242733
dc.descriptionA low protein diet has been shown to affect the amount and activity of several enzymes and to decrease insulin secretion by islets isolated from rats fed such a diet. To understand the mechanisms involved in this phenomenon, we investigated the effects of forskolin, a stimulator of adenylyl cyclase, on insulin secretion by pancreatic islets from rats fed a normal (17%; NP) or low (6%; LP) protein diet for 8 wk. Isolated islets were incubated for 1 h in Krebs-bicarbonate solution containing 8.3 mmol glucose/L, with or without 10 μmol forskolin/L. The forskolin-induced insulin secretion was higher in islets from NP rats than in those from LP rats (P < 0.05). Western blotting revealed that the amount of the α catalytic subunit of protein kinase A (PKAα) was 35% lower in islets from LP rats than in islets from NP rats (P < 0.05). Moreover, PKAα mRNA expression was reduced by 30% in islets from LP rats (P < 0.05). Our results indicated a possible relationship between a low protein diet and a reduction in PKAα expression. These alterations in PKAα may be responsible in part for the decreased insulin secretion by islets from rats fed a low protein diet.
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dc.languageen
dc.publisher
dc.relationJournal of Nutrition
dc.rightsfechado
dc.sourceScopus
dc.titleDecreased Insulin Secretion In Islets From Rats Fed A Low Protein Diet Is Associated With A Reduced Pkaα Expression
dc.typeArtículos de revistas


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