Morphological Characterization Of Anticarsia Gemmatalis M Nucleopolyhedrovirus Infection In Haemocytes From Its Natural Larval Host, The Velvet Bean Caterpillar Anticarsia Gemmatalis (hübner) (lepidoptera: Noctuidae)

For a better understanding of virus x host interactions, transmission electron microscopy was used to characterize the intrahaemocoelic infection of Anticarsia gemmatalis larval haemocytes by A. gemmatalis M nucleopolyhedrovirus (AgMNPV). At 12 h post-infection (h p.i.), we observed nuclear hypertrophy, budded virus assembling, and protrusion towards the cytoplasm, virion envelopment, and accumulation of fibrillar aggregates in the cytoplasm. Around 24 h p.i., fibrillar aggregates also appeared inside nuclei of infected cells. By 48 h p.i., virogenic stroma and polyhedra were visualised in nuclei and at 72 h p.i., widespread infection in haemocytes was observed. Cell remnants and free polyhedra were phagocytosed by granular haemocyte 1 and plasmatocytes. Entire cells were phagocytosed only by plasmatocytes. Necrosis of infected cells was quite common, suggesting a putative cytotoxic response. Granular haemocyte 1 presented a more exuberant protrusion of budded viruses in comparison to other haemocytes. All types of haemocytes were shown to be infected, and the intense virus replication in some of these cells reveals the importance of haemolymph for AgMNPV spread in its natural host, a critical factor for permissiveness. © 2004 Elsevier Ltd. All rights reserved.

171

180

Barrett, J.W., Brownwright, A.J., Primavera, M.J., Retnakaran, A., Palli, S.R., Concomitant primary infection of the midgut epithelial cells and the hemocytes of Trichoplusia ni by Autographa californica nucleopolyhedrovirus (1998) Tissue Cell, 30, pp. 602-616

Brehélin, M., Zachary, D., Insect haemocytes: A new classification to rule out the controversy (1986) Immunity in Invertebrates: Cells, Molecules and Defense Reactions, pp. 36-48. , Brehélin, M. (Ed.), Springer-Verlag, Berlin

Castro, M.E.B., Ribeiro, B.M., Production of viral progeny in insect cells undergoing apoptosis induced by a mutant Anticarsia gemmatalis nucleopolyhedrovirus (2001) Microbiol. Res., 156, pp. 369-376

Chernysh, S., Kim, S.I., Bekker, G., Pleskach, V.A., Filatova, N.A., Anikin, V.B., Platonov, V.G., Bulet, P., Antiviral and antitumor peptides from insects (2002) Proc. Natl. Acad. Sci. U.S.A., 99, pp. 12628-12632

Clark, T.E., Clem, R.J., Lack of involvement of haemocytes in the establishment and spread of infection in Spodoptera frugiperda larvae infected with the baculovirus Autographa californica M nucleopolyhedrovirus by intrahaemocolic injection (2002) J. Gen. Virol., 83, pp. 1565-1572

Clark, T.E., Clem, R.J., In vivo induction of apoptosis correlating with reduced infectivity during baculovirus infection (2003) J. Virol., 77, pp. 2227-2232

Clark, T.E., Clem, R.J., Insect defenses against virus infection: The role of apoptosis (2003) Int. Rev. Immunol., 22, pp. 401-424

Clem, R.J., Baculoviruses and apoptosis: The good, the bad and the ugly (2001) Cell Death Differ., 8, pp. 137-143

Granados, R.R., Infectivity and mode of action of baculoviruses (1980) Biotechnol. Bioeng., 22, pp. 1377-1405

Granados, R.R., Lawler, K.A., In vivo pathway of Autographa californica baculovirus invasion and infection (1981) Virology, 108, pp. 297-308

Granados, R.R., Guoxun, L., Dersksen, A.C.G., McKenna, K.A., A new insect cell line from Trichoplusia ni (BTI-Tn-5B1-4) susceptible to Trichoplusia ni single enveloped nuclear polyhedrosis virus (1994) J. Invert. Pathol., 64, pp. 260-266

Grasela, J.J., McIntosh, A.H., In vitro and in vivo host range of Anticarsia gemmatalis multiple nuclear polyhedrosis virus (1998) In Vitro Cell Dev. Biol. Anim., 34, pp. 79-83

Gupta, A.P., Insect immunocytes and other hemocytes: Roles in cellular and humoral immunity (1991) Immunology of Insects and Other Arthropods, pp. 19-118. , Gupta, A.P. (Ed.), CRC Press, Boca Raton

Hoover, K., Washburn, J.O., Volkman, L.E., Midgut-based resistance of Heliothis virescens to baculovirus infection mediated by phytochemicals in cotton (2000) J. Insect. Physiol., 46, pp. 999-1007

Johnson, D.W., Maruniak, J.E., Physical map of Anticarsia gemmatalis nuclear polyhedrosis virus (AgMNPV-2) DNA (1989) J. Gen. Virol., 70, pp. 1877-1883

Kislev, N., Harpaz, I., Zelcer, A., Electron-microscopic studies on haemocytes of the Egyptian conttonworm, Spodoptera litorallis (Boisduval) infected with a nuclear-polyhedrosis virus, as compared to noninfected hemocytes. II. Virus-infected hemocytes (1969) J. Invert. Pathol., 14, pp. 245-257

Lavine, M.D., Strand, M.R., Insect hemocytes and their role in immunity (2002) Insect Biochem. Mol. Biol., 32, pp. 1295-1309

Matos, T.G.T., Giugliano, L.G., Ribeiro, B.M., Báo, S.N., Structural and ultrastructural studies of Anticarsia gemmatalis midgut cells infected with the baculovirus A. gemmatalis nucleopolyhedrovirus (1999) Int. J. Insect Morphol. Embriol., 28, pp. 195-201

Moscardi, F., Assessment of the application of baculoviruses for control of lepidoptera (1999) Annu. Rev. Entomol., 44, pp. 257-289

O'Reilly, D.R., Miller, L.K., Luckow, V.A., Baculovirus Expression Vectors. A Laboratory Manual (1992), W.H. Freeman and Company, New YorkPinedo, F.J.R., Moscardi, F., Luque, T., Olszewski, J.A., Ribeiro, B.M., Inactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host (2003) Biol. Control, 27, pp. 336-344

Pombo, V., Velloso, L.M., Ribeiro, B.M., Báo, S.N., Structural and ultrastructural changes during the infection of UFL-AG-286 cells with the baculovirus AgMNPV (1998) J. Invert. Pathol., 72, pp. 239-245

Razuck, F.B., Ribeiro, B., Vargas, J.H., Wolff, J.L., Ribeiro, B.M., Characterization of the p10 gene region of Anticarsia gemmatalis nucleopolyhedrovirus (2002) Virus Genes, 24, pp. 243-247

Ribeiro, C., Simões, N., Brehélin, M., Insect immunity: The haemocytes of the armyworm Mythimna unipuncta (Lepidoptera: Noctuidae) and their role in defence reactions. In vivo and in vitro studies (1996) J. Insect. Physiol., 42, pp. 815-822

Ribeiro, B.M., Souza, M.L., Kitajima, E.W., Taxonomia, caracterização molecular e bioquímica de vírus de insetos (1998) Controle Microbiano de Insetos, pp. 481-507. , Alves, S.B. (Ed.) FEALQ, Piracicaba

Ribeiro, B.M., Gatti, C.D.C., Costa, M.H., Moscardi, F., Maruniak, J.E., Possee, R.D., Zanotto, P.M.A., Construction of a recombinant Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV-2D) harbouring the beta-galactosidase gene (2001) Arch. Virol., 146, pp. 1355-1367

Rodrigues, J.C., Souza, M.L., O'Reilly, D.R., Velloso, L.M., Pinedo, F.J., Razuck, F.B., Ribeiro, B., Ribeiro, B.M., Characterization of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (2001) Virus Genes, 22, pp. 103-112

Sieburth, P.J., Maruniak, J.E., Susceptibility of an established cell line of Anticarsia gemmatalis (Lepdoptera: Noctuidae) to three nuclear polyhedrosis viruses (1988) J. Invert. Pathol., 52, pp. 453-458

Silveira, E.B., Ribeiro, B.M., Báo, S.N., Morphological studies of apoptosis in insect cells infected with vApAg, an Anticarsia gemmatalis nucleopolyhedrovirus mutant (1999) J. Submicrosc. Cytol. Pathol., 31, pp. 543-554

Silveira, E.B., Ribeiro, B.M., Báo, S.N., Characterization of larval haemocytes from the velvetbean caterpillar Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae) (2003) J. Submicrosc. Cytol. Pathol., 35, pp. 129-139

Stettler, P., Wyler, T., Pfister-Wilhelm, R., Lanzrein, B., Overview of parasitism associated effects on host haemocytes in larval parasitoids and comparison with effects of the egg-larval parasitoid Chelonus inanitus on its host Spodoptera littoralis (1998) J. Insect. Physiol., 44, pp. 817-831

Trudeau, D., Washburn, J., Volkman, L.E., Central role of haemocytes in Autographa californica M nucleopolyhedrosis pathogenesis in Heliothis virescens and Helicoverpa zea (2001) J. Virol., 75, pp. 996-1003

Volkman, L.E., Keddie, B.A., Nuclear polyhedrosis virus pathogenesis (1990) Semin. Virol., 1, pp. 249-256

Washburn, J.O., Kirkpatrick, B.A., Volkman, L.E., Comparative pathogenesis of Autographa californica M nuclear polyhedrosis virus in larvae of Trichoplusia ni and Heliothis virescens (1995) Virology, 209, pp. 561-568

Washburn, J.O., Kirkpatrick, B.A., Volkman, L.E., Insect protection against viruses (1996) Nature, 383, p. 767

Washburn, J.O., Haas-Stapleton, E.J., Tan, F.F., Beckage, N.E., Volkman, L.E., Co-infection of Manduca sexta larvae with polydnavirus from Cotesia congregata increases susceptibility to fatal infection by Autographa californica M nucleopolyhedrovirus (2000) J. Insect. Physiol., 46, pp. 179-190

Williams, G.V., Faulkner, P., Cytological changes and viral morphogenesis during baculovirus infection (1997) The Baculoviruses, pp. 61-107. , Miller, L.K. (Ed.) Plenum Press, New York

Zhang, P., Yang, K., Daí, X., Pang, Y., Su, D., Infection of wild-type Autographa californica multicapsid nucleopolyhedrovirus induces in vivo apoptosis of Spodoptera litura larvae (2002) J. Gen. Virol., 83, pp. 3003-3011

Subjects

Show full item record