The Effects Of Copper Ions On The Synthesis Of Periplasmic And Membrane Proteins In Acidithiobacillus Ferrooxidans As Analyzed By Sds-page And 2d-page

dc.creatorFelicio A.P.
dc.creatorGarcia Jr. O.
dc.creatorBertolini M.C.
dc.creatorOttoboni L.M.M.
dc.creatorNovo M.T.M.
dc.date2003
dc.date2015-06-30T17:32:20Z
dc.date2015-11-26T14:11:27Z
dc.date2015-06-30T17:32:20Z
dc.date2015-11-26T14:11:27Z
dc.date.accessioned2018-03-28T21:11:59Z
dc.date.available2018-03-28T21:11:59Z
dc.identifier
dc.identifierHydrometallurgy. , v. 71, n. 01/02/15, p. 165 - 171, 2003.
dc.identifier0304386X
dc.identifier10.1016/S0304-386X(03)00153-1
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-17944384988&partnerID=40&md5=c9ddac782b6b15aaab99c28ae1a086b0
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/102536
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/102536
dc.identifier2-s2.0-17944384988
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1241732
dc.descriptionThe effects of 200 mM copper ions on the synthesis of membrane and periplasmic proteins were investigated in iron-grown cells of Acidithiobacillus ferrooxidans (At. ferrooxidans). Total membrane protein profiles of cells grown in the absence of copper ions (unadapted cells) and in the presence of copper ions (copper-adapted cells) were compared by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Crude preparations of outer membrane and periplasmic proteins were analyzed by SDS-PAGE. The synthesis of proteins was diminished or increased in the presence of copper ions. Low molecular weight proteins (<14 kDa) were significantly repressed by copper. These proteins are probably acidic proteins located in the outer membrane. An over-expression of a periplasmic protein of about 17 kDa was detected in the copper-adapted cells and was assumed to be rusticyanin, a 16.5-kDa periplasmic copper protein present in At. ferrooxidans cells and involved in the electron-transport chain of the iron oxidation pathway. To our knowledge, this is the first report of a possible involvement of the rusticyanin and outer membrane proteins in the mechanism of copper resistance in At. ferrooxidans. © 2003 Elsevier B.V. All rights reserved.
dc.description71
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dc.description171
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dc.languageen
dc.publisher
dc.relationHydrometallurgy
dc.rightsfechado
dc.sourceScopus
dc.titleThe Effects Of Copper Ions On The Synthesis Of Periplasmic And Membrane Proteins In Acidithiobacillus Ferrooxidans As Analyzed By Sds-page And 2d-page
dc.typeActas de congresos


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