Artículos de revistas
Isolation And Characterization Of A Lectin From Annona Muricata Seeds
Registro en:
Protein Journal. , v. 22, n. 07/08/15, p. 655 - 661, 2003.
15723887
2-s2.0-55449131874
Autor
Damico D.C.S.
Freire M.G.M.
Gomes V.M.
Toyama M.H.
Marangoni S.
Novello J.C.
Macedo M.L.R.
Institución
Resumen
A lectin with a high affinity for glucose/mannose was isolated from Annona muricata seeds (Annonaceae) by gel filtration chromatography on Sephacryl S-200, ion exchange chromatography on a DEAE SP-5 PW column, and molecular exclusion on a Protein Pak Glass 300 SW column. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (PAGE) yielded two protein bands of approximately 14 kDa and 22 kDa. However, only one band was seen in native PAGE. The Mr of the lectin estimated by fast-performance liquid chromatography-gel filtration on Superdex 75 was 22 kDa. The lectin was a glycoprotein with 8% carbohydrate (neutral sugar) and required divalent metal cations (Ca2+, Mg 2+, and Mn2+) for full activity. Amino acid analysis revealed a large content of Glx, Gly, Phe, and Lys. The lectin agglutinated dog, chicken, horse, goose, and human erythrocytes and inhibited the growth of the fungi Fusarium oxysporum, Fusarium solani, and Colletotrichum musae. © 2003 Plenum Publishing Corporation. 22 07/08/15 655 661 Allen, A.K., Neuberger, A., Sharon, N., (1973) Biochem. J., 131, pp. 155-162 Bones, C., Loiseau, P., Cortes, D., Myint, S.H., Hocquemiller, R., Gayral, P., (1991) Plant Med., 57, pp. 434-436 Bradford, M.M., (1976) Anal. Biochem., 72, pp. 248-254 Branch, L.C., Silva, I.M.F., (1983) Acta Amazônica., 13, pp. 737-797 Coelho, M.B., Freire, M.G.M., Toyama, M.H., Marangoni, S., Novello, J.C., Macedo, M.L.R., (2003) Protein and Peptide Letter, 10, pp. 1-9 Datta, P.K., Figueroa, M.O.D.C.R., Lajolo, F.M., (1991) Plant Physiol., 97, pp. 856-862 Dubois, M., Gilles, K.A., Hamilton, J.L., Rebers, P.A., Smith, F., (1956) Anal. Chem, 28, pp. 350-356 Freire, M.G.M., Gomes, V.M., Corsini, R.E., Machado, O.L.T., De Simone, S.G., Novello, J.C., (2002) Plant Physiol. Biochem., 40, pp. 61-68 Goldstein, I.J., Hughes, R.C., Monsigny, M., Osawa, T., Sharon, N., (1980) Nature, 285, pp. 66-73 Gomes, V.M., Da Cunha, M., Miguens, F.C., Fernandes, K.V.S., Rose, T.L., Xavier-Filho, J., (1998) Plant Sci., 138, pp. 81-89 Henrikson, R.L., Meredith, S.C., (1984) Anal. Biochem., 136, pp. 65-71 Laemmli, U.K., (1970) Nature, 227, pp. 680-685 Lis, H., Sharon, N., (1984) Biology of Carbohydrates, pp. 1-85. , Ginsburg, V., and Robbins, P. W. (eds), Wiley, New York Lis, H., Sharon, N., (1986) Annu. Rev. Biochem., 55, pp. 35-67 Moreira, R.A., Monteiro, A.C.O., Horta, A.C.G., Oliveira, J.T.A., Cavada, B.S., (1997) Phytochemistry, 2, pp. 139-144 Moreira, R.A., Castelo-Branco, C.C., Monteiro, A.C.O., Tavares, R.O., Beltramini, L.M., (1998) Phytochemistry, 47, pp. 1183-1188 Peumans, W.J., Verhaert, P., Pfüller, U., Van Damme, E.J.M., (1984) FEBS Lett., 177, pp. 261-265 Sampietro, A.R., Isla, M.I., Quiroga, E.N., Vattuone, M.A., (2001) Plant Sci., 160, pp. 659-667 Sharon, N., Lis, H., (1990) FASEB J., 4, pp. 3198-3208 Shibuya, N., Goldstein, I.J., Shafer, J.A., Peumans, W.J., Broekaert, W.F., (1986) Arch. Biochem. Biophys., 249, pp. 215-224 Singh, R.S., Tiwary, A.K., Kennedy, J.F., (1999) Crit. Rev. Biotechnol., 19, pp. 145-178 Utarabhand, P., Akkayamont, P., (1995) Phytochemistry, 38, pp. 281-285 Van Damme, E.J.M., Peumans, W.J., Barre, A., Rougé, P., (1998) Crit. Rev. Plant. Sci., 17, pp. 575-692 Van Parijs, J., Broekaert, W.F., Goldstein, I.J., Peumans, W.J., (1991) Planta, 183, pp. 258-264 Van Parijs, J., Broekaert, W.F., Peumans, W.J., Geuns, J.M., Van Laere, A.J., (1992) Arch. Microbiol., 158, pp. 19-25 Wang, H.X., Ng, T.B., Ooi, V.E.C., (1999) Int. J. Biochem. Cell Biol., 31, pp. 595-599 Xu, Q., Lu, Y., Wang, X., Gu, H., Chen, Z., (1998) Plant Physiol. Biochem., 36, pp. 899-905