Relationship Between Phophatase Activity And Cytotoxic Effect Of Two Protein Phosphatase Inhibitors, Okadaic Acid And Pervanadate, On Human Myeloid Leukemia Cell Line

dc.creatorGalvao Freire A.C.
dc.creatorAoyama H.
dc.creatorHaun M.
dc.creatorFerreira C.V.
dc.date2003
dc.date2015-06-30T17:31:05Z
dc.date2015-11-26T14:10:03Z
dc.date2015-06-30T17:31:05Z
dc.date2015-11-26T14:10:03Z
dc.date.accessioned2018-03-28T21:10:41Z
dc.date.available2018-03-28T21:10:41Z
dc.identifier
dc.identifierJournal Of Enzyme Inhibition And Medicinal Chemistry. , v. 18, n. 5, p. 425 - 429, 2003.
dc.identifier14756366
dc.identifier10.1080/147563031000155634
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0141761405&partnerID=40&md5=c9327bde306a5a0cf6495c46450271fa
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/102412
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/102412
dc.identifier2-s2.0-0141761405
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1241409
dc.descriptionProtein phosphatases are signalling molecules that regulate a variety of fundamental cellular processes including cell growth, metabolism and apoptosis. The aim of this work was to correlate the cytotoxicity of pervanadate and okadaic acid on HL60 cells and their effect on the phosphatase obtained from these cells. The cytotoxicity of these protein phosphatase inhibitors was evaluated on HL60 cells using phosphatase activity, protein quantification and MTT reduction as indices. The major phosphatase presents in the cellular extract showed high activity (80%) and affinity (Km = 0.08 mM) to tyrosine phosphate in relation to p-nitrophenyl phosphate (pNPP) - (Km = 0.51 mM). Total phosphatase (pNPP) was inhibited in the presence of 10 mM vanadate (98%), 200 μM pervanadate (95%) and 100 μM p-chloromercuribenzoate (80%) but okadaic acid caused a slight increase in enzyme activity (25%). When the HL60 cells were treated with the phosphatase inhibitors (pervanadate and okadaic acid) for 24 hours, only 20% residual activity was observed in presence of 200 μM pervanadate, whereas in the presence of okadaic acid this inhibitory effect was not observed. However, in respect to mitochondrial function, cell viability decreased about 80% in the presence of 100 nM okadaic acid. The total protein content was decreased 25% when the cells were treated with 100 nM okadaic acid in combination with 200 μM pervanadate. Our results suggest that both phosphatase inhibitors presented different mechanisms of action on HL60 cells. However, their effect on the cell redox status have to be considered.
dc.description18
dc.description5
dc.description425
dc.description429
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dc.languageen
dc.publisher
dc.relationJournal of Enzyme Inhibition and Medicinal Chemistry
dc.rightsfechado
dc.sourceScopus
dc.titleRelationship Between Phophatase Activity And Cytotoxic Effect Of Two Protein Phosphatase Inhibitors, Okadaic Acid And Pervanadate, On Human Myeloid Leukemia Cell Line
dc.typeArtículos de revistas


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