Artículos de revistas
Dexamethasone And Fetal Calf Serum Effects In Differentiation Of Vero Cells Cultured On Type I Collagen Gel
Registro en:
Journal Of Submicroscopic Cytology And Pathology. , v. 35, n. 1, p. 35 - 42, 2003.
11229497
2-s2.0-0642272488
Autor
Santos Jr. A.R.
Dolder H.
Wada M.L.F.
Institución
Resumen
Vero cells, a fibroblastic cell line, were cultured on a collagen I gel without fetal calf serum (FCS), with the addition of 10% FCS, 10% FCS plus dexamethasone (DEX) or 20% FCS. Our objective was to determine the effects of DEX on the differentiation pattern of fibroblastic cells cultured on a collagen substrate. We found that cells cultured with or without 10% FCS were capable of migrating into the collagen matrix. Near the cells in the gel, we found the deposition of extracellular granulations. Cytochemical data suggests that this material is glycosaminoglycans and/or proteoglycans. Surrounding the cells, a fibronectin deposition was found in the collagen. Thus, these cells make up a structure similar to a loose connective tissue. On the other hand, cells cultured with 10% FCS plus DEX or with 20% FCS did not invade the collagen matrix but formed multiple cell layers poor in fibronectin. 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