Biochemical, Pharmacological And Structural Characterization Of A New Pla2 From Crotalus Durissus Terrificus (south American Rattlesnake) Venom

dc.creatorHernandez-Oliveira S.
dc.creatorToyama M.H.
dc.creatorToyama D.O.
dc.creatorMarangoni S.
dc.creatorHyslop S.
dc.creatorRodrigues-Simioni L.
dc.date2005
dc.date2015-06-26T14:09:03Z
dc.date2015-11-26T14:08:44Z
dc.date2015-06-26T14:09:03Z
dc.date2015-11-26T14:08:44Z
dc.date.accessioned2018-03-28T21:09:18Z
dc.date.available2018-03-28T21:09:18Z
dc.identifier
dc.identifierProtein Journal. , v. 24, n. 4, p. 233 - 242, 2005.
dc.identifier15723887
dc.identifier10.1007/s10930-005-6718-z
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-27744443755&partnerID=40&md5=9b5ef28529b53429a0b96cf65cd949b3
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/93694
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/93694
dc.identifier2-s2.0-27744443755
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1241072
dc.descriptionA new PLA2 (F16) was purified from Crotalus durissus terrificus venom by molecular exclusion chromatography followed by analytical reverse phase HPLC. The PLA2 (14.86 kDa by MALDI-TOF mass spectrometry) had an amino acid sequence of SLLQFNKMIKFETRKNAVPFYAFYGCYCGWGGRRRPKDATDRCCFVHDCCYEKVTKCNTKWD IYRYSLKSGYITCGKGTWCKEQICECDRVAAECLRRSLSTYKNGYMFYPDSRCRGPSETC, and showed highly conserved Ca2+-binding and catalytic sites. F16 showed allosteric behavior with 10 mM Ca2+ and had temperature and pH optima of 25°C and 7.9, respectively. F16 (10 μg/ml) produced neuromuscular blockade in chick biventer cervicis preparations in the absence and presence of crotapotin, indicating that crotapotin was not essential for neuromuscular action in this preparation. In contrast, in mouse phrenic nerve-diaphragm preparations, the neuromuscular blockade produced by the same concentration of toxin was dependent on crotapotin. Pre-incubation with heparin markedly reduced the neurotoxicity of F16. These results show that the biochemical and structural properties of F16 are similar to those of the PLA2 isoforms F15 and F17, but that the neurotoxicity and the requirement for crotapotin to form the crotoxin complex varies according to the neuromuscular preparation. © 2005 Springer Science+Business Media, Inc.
dc.description24
dc.description4
dc.description233
dc.description242
dc.descriptionAraújo, F.A.A., Santalúcia, M., Cabral, R.F., Cardoso, J.L.C., França, F.O.S., Fan, H.W., Málaque, C.M.S., Haddad Jr., V., Epidemioligia dos Acidentes por Animais Peçonhentos. (2003) Animais Peçonhentos No Brasil. Biologia, Clínica e Terapêutica Dos Acidentes, pp. 6-12. , Sarvier/FAPESP São Paulo
dc.descriptionAzevedo-Marques, M.M., Hering, S.E., Cupo, P., Cardoso, J.L.C., França, F.O.S., Fan, H.W., Málaque, C.M.S., Haddad Jr., V., Acidente Crotálico. (2003) Animais Peçonhentos No Brasil. Biologia, Clínica e Terapêutica Dos Acidentes, pp. 91-98. , Sarvier/FAPESP São Paulo
dc.descriptionBeghini, D.G., Hernandez-Oliveira, S., Rodrigues-Simioni, L., Novello, J.C., Hyslop, S., Marangoni, S., (2004) Toxicon, 44, pp. 141-148
dc.descriptionBeghini, D.G., Rodrigues-Simioni, L., Toyama, M.H., Novello, J.C., Da Cruz-Höfling, M.A., Marangoni, S., (2004) Toxicon, 43, pp. 255-261
dc.descriptionBeghini, D.G., Toyama, M.H., Hyslop, S., Sodek, L., Novello, J.C., Marangoni, S., (2000) J. Protein Chem., 19, pp. 603-607
dc.descriptionBon, C., Bouchier, C., Choumet, V., Faure, G., Jiang, M.S., Lambezat, M.P., Radvanyi, F., Saliou, B., (1989) Acta Physiol. Pharmacol. Latinoam., 39, pp. 439-448
dc.descriptionBonventre, J.V., Sapirstein, A., (2002) Adv. Exp. Med. Biol., 507, pp. 25-31
dc.descriptionBülbring, E., (1946) Br. J. Pharmacol., 1, pp. 38-61
dc.descriptionChoumet, V., Bouchier, C., Delot, E., Faure, G., Saliou, B., Bon, C., (1996) Adv. Exp. Med. Biol., 391, pp. 197-202
dc.descriptionDennis, E.A., (1994) J. Biol. Chem., 269, pp. 13057-13060
dc.descriptionFaure, G., Bon, C., (1987) Toxicon, 25, pp. 229-234
dc.descriptionFaure, G., Bon, C., (1988) Biochemistry, 27, pp. 730-738
dc.descriptionFaure, G., Choumet, V., Bouchier, C., Camoin, L., Guillaume, J.L., Monegier, B., Vuilhorgne, M., Bon, C., (1994) Eur. J. Biochem., 223, pp. 161-164
dc.descriptionFaure, G., Guillaume, J.L., Camoin, L., Saliou, B., Bon, C., (1991) Biochemistry, 30, pp. 8074-8083
dc.descriptionFaure, G., Harvey, A.L., Thomson, E., Saliou, B., Radvanyi, F., Bon, C., (1993) Eur. J. Biochem., 214, pp. 491-496
dc.descriptionGinsborg, B.L., Warriner, J., (1960) Br. J. Pharmacol., 15, pp. 410-421
dc.descriptionGutiérrez, J.M., Lomonte, B., (1995) Toxicon, 33, pp. 1405-1424
dc.descriptionGutiérrez, J.M., Ownby, C.L., (2003) Toxicon, 42, pp. 915-931
dc.descriptionHabermann, E., Breithaupt, H., (1978) Toxicon, 16, pp. 19-30
dc.descriptionHendon, R.A., Fraenkel-Conrat, H., (1971) Proc. Natl. Acad. Sci. USA, 68, pp. 1560-1563
dc.descriptionHolzer, M., MacKessy, S.P., (1996) Toxicon, 34, pp. 1149-1155
dc.descriptionKini, R.M., (2003) Toxicon, 42, pp. 827-840
dc.descriptionKini, R.M., Evans, H.J., (1989) Toxicon, 7, pp. 613-635
dc.descriptionKudo, I., Murakami, M., (2002) Prostag. Oth. Lipid Mediat., 68, pp. 3-58
dc.descriptionLambeau, G., Lazdunski, M., (1999) Trends Pharmacol. Sci., 20, pp. 162-170
dc.descriptionLennon, B.W., Kaiser, I.I., (1990) Comp. Biochem. Physiol. B, 97, pp. 695-699
dc.descriptionLomonte, B., Angulo, Y., Calderón, L., (2003) Toxicon, 42, pp. 885-901
dc.descriptionMayer, R.J., Marshall, L.A., (1993) FASEB J., 7, pp. 339-348
dc.descriptionOliveira, D.G., Toyama, M.H., Novello, J.C., Beriam, L.O., Marangoni, S., (2002) J. Protein Chem., 21, pp. 161-168
dc.descriptionPonce-Soto, L.A., Toyama, M.H., Hyslop, S., Novello, J.C., Marangoni, S., (2002) J. Protein Chem., 21, pp. 131-136
dc.descriptionRangel-Santos, A., Dos-Santos, E.C., Lopes-Ferreira, M., Lima, C., Cardoso, D.F., Mota, I., (2004) Toxicon, 43, pp. 801-810
dc.descriptionSelistre-De-Araujo, H., White, S.P., Ownby, C.L., (1996) Arch. Biochem. Biophys., 326, pp. 21-30
dc.descriptionSoares, A.M., Giglio, J.R., (2003) Toxicon, 42, pp. 855-868
dc.descriptionToyama, M.H., Oliveira, D.G., Beriam, L.O.S., Novello, J.C., Rodrigues-Simioni, L., Marangoni, S., (2003) Toxicon, 41, pp. 1033-1045
dc.descriptionToyama, M.H., Soares, A.M., Vieira, C.A., Novello, J.C., Oliveira, B., Giglio, J.R., Marangoni, S., (1998) J. Protein Chem., 17, pp. 713-718
dc.descriptionToyama, M.H., Soares, A.M., Wen-Hwa, L., Polikarpov, I., Giglio, J.R., Marangoni, S., (2000) Biochimie, 82, pp. 245-250
dc.languageen
dc.publisher
dc.relationProtein Journal
dc.rightsfechado
dc.sourceScopus
dc.titleBiochemical, Pharmacological And Structural Characterization Of A New Pla2 From Crotalus Durissus Terrificus (south American Rattlesnake) Venom
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución