Purification Of Betulinic Acid From Eugenia Florida (myrtaceae) By High-speed Counter-current Chromatography

dc.creatorFrighetto N.
dc.creatorWelendorf R.M.
dc.creatorPereira da Silva A.M.
dc.creatorNakamura M.J.
dc.creatorSiani A.C.
dc.date2005
dc.date2015-06-26T14:08:02Z
dc.date2015-11-26T14:07:35Z
dc.date2015-06-26T14:08:02Z
dc.date2015-11-26T14:07:35Z
dc.date.accessioned2018-03-28T21:08:15Z
dc.date.available2018-03-28T21:08:15Z
dc.identifier
dc.identifierPhytochemical Analysis. , v. 16, n. 6, p. 411 - 414, 2005.
dc.identifier9580344
dc.identifier10.1002/pca.860
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-28244450307&partnerID=40&md5=833fcee35c86bd1db0c85d277aaa227f
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/93487
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/93487
dc.identifier2-s2.0-28244450307
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1240816
dc.descriptionA high yield of betulinic acid (up to 17% from the ethanolic extract) was found in the leaves of Eugenia florida collected in south-eastern Brazil, making this species a potential commercial source of the title compound. Extracts of E. florida were subjected to solvent partition, and rapid high-speed counter-current chromatography (HSCCC) was applied to the semi-crude extracts to afford betulinic acid in high purity. The mobile and stationary phases were derived from the two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (10:5:2.5:1). The developing solvent system (stationary and mobile phases) for optimum HSCCC separation was chosen by dissolving the fraction to be chromatographed in the proposed solvent mixture and determining the amount of betulinic acid in each phase by densitometric TLC. Purified betulinic acid was characterized by 13C-NMR, GC-MS and co-injection of its methyl ester with standards in GC-FID. The HSCCC technique is commonly employed to isolate triterpene glycosides, but is applied in this study to an aglycone. Copyright © 2005 John Wiley & Sons, Ltd.
dc.description16
dc.description6
dc.description411
dc.description414
dc.descriptionBringmann, G., Saeb, W., Assi, L.A., François, G., Narayanan, A.S.S., Peters, E.-M., Betulinic acid: Isolation from Triphyophyllum peltatum and Ancistrocladus heyneanus, anti-malarial activity and crystal structure of the benzyl ester (1997) Planta Med., 63, pp. 255-257
dc.descriptionBudzikiewicz, H., Wilson, J.M., Djerassi, C., Mass spectrometry in structural and stereochemical problems. XXXII. Pentacyclic triterpenes (1963) J. Am. Chem. Soc., 85, pp. 3688-3699
dc.descriptionConway, W.D., (1990) Countercurrent Chromatography: Apparatus, Theory and Applications, , VCH-Wiley: New York
dc.descriptionDräger, D., Neubert, R., Galgon, T., Wohlrab, W., Verfahren zur Extraktion von Betulinsäure aus der Borke von Platanus acerifolia unter Nutzung mitterpolarer Lösungsmittel (2001), Ger Offen DE 19,713,768Fujioka, T., Kashiwada, Y., Kilkuskie, R.E., Cosentino, L.M., Ballas, L.M., Jiang, J.B., Janzen, W.P., Lee, K.-H., Anti-AIDS agents, 11. Betulinic acid and platanic acid as anti-HIV principles from Syzigium claviflorum, and the anti-HIV activity of structurally related triterpenoids (1994) J. Nat. Prod., 57, pp. 243-247
dc.descriptionHayek, E.W.H., Jordis, U., Moche, W., Sauter, F., A bicentennial of betulin (1989) Phytochemistry, 28, pp. 2229-2242
dc.descriptionHostettmann, K., Marston, A., Countercurrent chromatography in the preparative separation of plant-derived natural products (2001) J. Liq. Chrom. Relat. Technol., 24, pp. 1711-1721
dc.descriptionIto, Y., Oka, H., Kitazume, E., Bhatnagar, M., Lee, Y.W., Improved high-speed countercurrent chromatograph with three multilayer coils connected in series. III. Evaluation of semianalytical column with various biological samples and inorganic elements (1990) J. Liq. Chromatogr., 3, pp. 2329-2349
dc.descriptionJunges, M.J., Fernandes, J.B., Rodrigues-Filho, E., Vieira, P.C., Silva, M.F.G.F., Potenciais anti-AIDS isolados de Eugenia florida (1994) XIII Simpósio De Plantas Medicinais Do Brasil, 1, p. 135. , Anais do Congresso
dc.descriptionKim, D.S.H.L., Chen, Z., Nguyen, V.T., Pezzuto, J.M., A concise semi-synthetic approach to betulinic acid from betulin (1997) Synth. Commun., 29, pp. 1607-1612
dc.descriptionMabberley, D.J., (1998) The Plant Book - A Portable Dictionary of the Vascular Plants, , (2nd edn). Cambridge University Press: London
dc.descriptionMarston, A., Hostettmann, K., Counter-current chromatography as a preparative tool - Applications and perspectives (1994) J. Chromatogr. A, 658, pp. 315-341
dc.descriptionPisha, E., Chai, H., Lee, I.-S., Chagwedera, T.E., Farnsworth, N.R., Cordell, G.A., Beecher, C.W.W., Pezzuto, J.M., Discovery of betulinic acid as a selective inhibitor of human melanoma that functions by induction of apoptosis (1995) Nature Med., 1, pp. 1046-1051
dc.descriptionSun, I.-C., Shen, J.-K., Wang, H.-K., Cosentino, L.M., Lee, K.-H., Anti-AIDS agents. 32. Synthesis and anti-AIDS activity of betulin derivatives (1998) Bioorg. Med. Chem. Lett., 8, pp. 1267-1272
dc.descriptionWagner, H., Bladt, S., (1996) Plant Drug Analysis, , 2nd edn. Springer: Berlin
dc.languageen
dc.publisher
dc.relationPhytochemical Analysis
dc.rightsfechado
dc.sourceScopus
dc.titlePurification Of Betulinic Acid From Eugenia Florida (myrtaceae) By High-speed Counter-current Chromatography
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución