Liquid-liquid extraction of biomolecules in downstream processing - A review paper

dc.creatorSilva, M. E.
dc.creatorFranco, T. T.
dc.date2000-03-01
dc.date2014-07-17T17:37:44Z
dc.date2015-11-26T13:20:42Z
dc.date2014-07-17T17:37:44Z
dc.date2015-11-26T13:20:42Z
dc.date.accessioned2018-03-28T21:04:07Z
dc.date.available2018-03-28T21:04:07Z
dc.identifierBrazilian Journal of Chemical Engineering. Brazilian Society of Chemical Engineering, v. 17, n. 1, p. 1-17, 2000.
dc.identifier0104-6632
dc.identifierS0104-66322000000100001
dc.identifier10.1590/S0104-66322000000100001
dc.identifierhttp://dx.doi.org/10.1590/S0104-66322000000100001
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322000000100001
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/26070
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/26070
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1239733
dc.descriptionEconomic analysis shows that protein separation and purification are a very important aspect of biomolecules production and processing. This is particularly true for protein processing which, because of the complexity of the starting material, often requires many steps to reach the levels of purity required for medical and food applications. The separation specialists' task is to develop safe and simple processes to achieve products with a high level of purity. On a large scale, chromatography of proteins is not an easily applied method, although on a laboratory scale it is very effective and relatively simple. When it is scaled up, shortcomings such as discontinuity in the process, slow protein diffusion and large pressure drops in the system are seen. For these reasons a substantial research effort has been directed toward the use of aqueous two-phase systems (ATPSs) to replace the initial steps in protein purification and chromatography. This article reviews the chronology and main ATPS fundamentals and discuss the broader applications of this type of system in the extraction and separation of biomolecules.
dc.description1
dc.description17
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherBrazilian Society of Chemical Engineering
dc.relationBrazilian Journal of Chemical Engineering
dc.rightsaberto
dc.sourceSciELO
dc.subjectaqueous two-phase systems
dc.subjectdownstream processing
dc.subjectprotein purification
dc.subjectliquid-liquid extraction
dc.titleLiquid-liquid extraction of biomolecules in downstream processing - A review paper
dc.typeArtículos de revistas


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