Aprotinin recovery: comparison between biospecific and pseudobiospecific affinity adsorptions

dc.creatorTAMAGAWA, R. E.
dc.creatorAZZONI, A. R.
dc.creatorMIRANDA, E. A.
dc.creatorVIJAYALAKSHMI, M. A.
dc.date1999-06-01
dc.date2014-07-17T17:37:43Z
dc.date2015-11-26T13:20:39Z
dc.date2014-07-17T17:37:43Z
dc.date2015-11-26T13:20:39Z
dc.date.accessioned2018-03-28T21:03:59Z
dc.date.available2018-03-28T21:03:59Z
dc.identifierBrazilian Journal of Chemical Engineering. Brazilian Society of Chemical Engineering, v. 16, n. 2, p. 119-127, 1999.
dc.identifier0104-6632
dc.identifierS0104-66321999000200003
dc.identifier10.1590/S0104-66321999000200003
dc.identifierhttp://dx.doi.org/10.1590/S0104-66321999000200003
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321999000200003
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/26064
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/26064
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1239698
dc.descriptionTwo adsorption techniques were studied as potential methods for the recovery of aprotinin: a biospecific adsorption (using immobilized trypsin and chymotrypsin as ligants) and a pseudobiospecific adsorption of aprotinin-trypsin complex onto IMAC matrix. These studies indicated that ionic strength has an important effect on aprotinin adsorption on immobilized trypsin, and the pH was the most significant variable in aprotinin desorption from both immobilized enzymes. The aprotinin-trypsin complex adsorption onto the IMAC matrix was not as significantly affected by changes in pH as it was for changes in ionic strength. For the desorption step, both variables significantly affected the complex recovery. However, the effect of ionic strength was markedly stronger for this desorption step.
dc.description119
dc.description127
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherBrazilian Society of Chemical Engineering
dc.relationBrazilian Journal of Chemical Engineering
dc.rightsaberto
dc.sourceSciELO
dc.subjectAprotinin recovery
dc.subjectadsorptions
dc.subjectIMAC matrix
dc.titleAprotinin recovery: comparison between biospecific and pseudobiospecific affinity adsorptions
dc.typeArtículos de revistas


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