Platelet antibody detection by flow cytometry: an effective method to evaluate and give transfusional support in platelet refractoriness

dc.creatorBub, Carolina Bonet
dc.creatorMartinelli, Beatriz Moraes
dc.creatorAvelino, Thayna Mendonca
dc.creatorGoncalez, Ana Claudia
dc.creatorBarjas-Castro, Maria de Lourdes
dc.creatorCastro, Vagner
dc.date2013-01-01
dc.date2014-07-17T17:54:28Z
dc.date2015-11-26T11:57:00Z
dc.date2014-07-17T17:54:28Z
dc.date2015-11-26T11:57:00Z
dc.date.accessioned2018-03-28T20:59:53Z
dc.date.available2018-03-28T20:59:53Z
dc.identifierRevista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e daSociedade Brasileira de Transplante de Medula Óssea, v. 35, n. 4, p. 252-255, 2013.
dc.identifier1516-8484
dc.identifierS1516-84842013000400252
dc.identifier10.5581/1516-8484.20130062
dc.identifierhttp://dx.doi.org/10.5581/1516-8484.20130062
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842013000400252
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/27313
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/27313
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1238644
dc.descriptionBACKGROUND: Immune platelet refractoriness is mainly caused by human leukocyte antigen antibodies (80-90% of cases) and, to a lesser extent, by human platelet antigen antibodies. Refractoriness can be diagnosed by laboratory tests and patients should receive compatible platelet transfusions. A fast, effective and low cost antibody-screening method which detects platelet human leukocyte/platelet antigen antibodies is essential in the management of immune platelet refractoriness.OBJECTIVE: The aim of this study was to evaluate the efficiency of the flow cytometry platelet immunofluorescence test to screen for immune platelet refractoriness.METHODS: A group of prospective hematologic patients with clinically suspected platelet refractoriness treated in a referral center in Campinas, SP during July 2006 and July 2011 was enrolled in this study. Platelet antibodies were screened using the flow cytometry platelet immunofluorescence test. Anti-human leukocyte antigen antibodies were detected by commercially available methods. The sensitivity, specificity and predictive values of the immunofluorescence test were determined taking into account that the majority of antiplatelet antibodies presented human leukocyte antigen specificity.RESULTS: Seventy-six samples from 32 female and 38 male patients with a median age of 43.5 years (range: 5-84 years) were analyzed. The sensitivity of the test was 86.11% and specificity 75.00% with a positive predictive value of 75.61% and a negative predictive value of 85.71%. The accuracy of the method was 80.26%.CONCLUSION: This study shows that the flow cytometry platelet immunofluorescence test has a high correlation with the anti-human leukocyte antigen antibodies. Despite a few limitations, the method seems to be efficient, fast and feasible as the initial screening for platelet antibody detection and a useful tool to crossmatch platelets for the transfusional support of patients with immune platelet refractoriness.
dc.description252
dc.description255
dc.languageen
dc.publisherAssociação Brasileira de Hematologia e Hemoterapia e daSociedade Brasileira de Transplante de Medula Óssea
dc.relationRevista Brasileira de Hematologia e Hemoterapia
dc.rightsaberto
dc.sourceSciELO
dc.subjectBlood platelets
dc.subjectAntigens, human leukocyte
dc.subjectFlow cytometry
dc.subjectHistocompatibility
dc.subjectAntigens, human platelet
dc.titlePlatelet antibody detection by flow cytometry: an effective method to evaluate and give transfusional support in platelet refractoriness
dc.typeArtículos de revistas


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