Effects of different general anesthetics on serum hemolysis and hepatic and muscular glycogenolysis in rats

dc.creatorMachado, E.F.A.
dc.creatorNormand, A.C.R.
dc.creatorNunes, L.A.S.
dc.creatorBrenzikofer, R.
dc.creatorMacedo, D.V.
dc.date2009-11-01
dc.date2014-07-18T13:28:47Z
dc.date2015-11-26T11:43:21Z
dc.date2014-07-18T13:28:47Z
dc.date2015-11-26T11:43:21Z
dc.date.accessioned2018-03-28T20:47:01Z
dc.date.available2018-03-28T20:47:01Z
dc.identifierBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 42, n. 11, p. 1035-1038, 2009.
dc.identifier0100-879X
dc.identifierS0100-879X2009001100006
dc.identifier10.1590/S0100-879X2009005000030
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2009005000030
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100006
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/31258
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/31258
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1235286
dc.descriptionAnesthetics can affect the structure and biological function of tissues and systems differentially. The aim of the present study was to compare three injectable anesthetics generally used in experiments with animals in terms of the degree of hemolysis and glycogenolysis occurring after profound anesthesia. Twenty-four male Wistar rats (330-440 g) were divided into three groups (N = 8): chloral hydrate (CH), ketamine + xylazine (KX), Zoletil 50® (zolazepam and tiletamine) + xylazine (ZTX). After deep anesthesia, total blood was collected. The liver and white (WG) and red gastrocnemius (RG) muscles were also immediately removed. The degree of serum hemolysis was quantified on the basis of hemoglobin concentration (g/L). Hepatic and muscular glycogen concentrations (mmol/kg wet tissue) were quantified by the phenol-sulfuric method. The CH and KX groups exhibited serum hemolysis (4.0 ± 2.2 and 1.9 ± 0.9 g/L, respectively; P < 0.05) compared to the ZTX group, which presented none. Only KX induced elevated glycogenolysis (mmol/kg wet tissue) in the liver (86.9 ± 63.2) and in WG (18.7 ± 9.0) and RG (15.2 ± 7.2; P < 0.05). The CH and ZTX groups exhibited no glycogenolysis in the liver (164.4 ± 41.1 and 176.8 ± 54.4, respectively), WG (28.8 ± 4.4, 32.0 ± 6.5, respectively) or RG (29.0 ± 4.9; 25.3 ± 8.6, respectively). Our data indicate that ZTX seems to be an appropriate general anesthetic for studies that seek to simultaneously quantify the concentration of glycogen and serum biochemical markers without interferences. ZTX is reasonably priced, found easily at veterinary markets, quickly induces deep anesthesia, and presents a low mortality rate.
dc.description1035
dc.description1038
dc.languageen
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relationBrazilian Journal of Medical and Biological Research
dc.rightsaberto
dc.sourceSciELO
dc.subjectGeneral anesthetic
dc.subjectRats
dc.subjectGlycogenolysis
dc.subjectHemolysis
dc.subjectZoletil
dc.subjectKetamine
dc.titleEffects of different general anesthetics on serum hemolysis and hepatic and muscular glycogenolysis in rats
dc.typeArtículos de revistas


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