Evaluation of histological changes after tracheal occlusion at different gestational ages in a fetal rat model

dc.creatorGallindo, Rodrigo Melo
dc.creatorGonçalves, Frances Lilian Lanhellas
dc.creatorBarreto, Carolina Teixeira de Resende
dc.creatorSchmidt, Augusto Frederico Santos
dc.creatorPereira, Luis Antonio Violin Dias
dc.creatorSbragia, Lourenço
dc.date2013-01-01
dc.date2014-07-18T18:04:23Z
dc.date2015-11-26T11:42:34Z
dc.date2014-07-18T18:04:23Z
dc.date2015-11-26T11:42:34Z
dc.date.accessioned2018-03-28T20:46:16Z
dc.date.available2018-03-28T20:46:16Z
dc.identifierClinics. Faculdade de Medicina / USP, v. 68, n. 1, p. 59-63, 2013.
dc.identifier1807-5932
dc.identifierS1807-59322013000100010
dc.identifier10.6061/clinics/2013(01)OA09
dc.identifierhttp://dx.doi.org/10.6061/clinics/2013(01)OA09
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322013000100010
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/35388
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/35388
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1235092
dc.descriptionOBJECTIVES: To evaluate the histological changes of tracheal cartilage and epithelium caused by tracheal occlusion at different gestational ages in a fetal rat model. METHODS: Rat fetuses were divided into two groups: a) External control, composed of non-operated rats, and b) Interventional group, composed of rats operated upon on gestational day 18.5 (term = 22 days), divided into triads: 1) Tracheal occlusion, 2) Internal control and 3) Sham (manipulated but not operated). Morphological data for body weight, total lung weight and total lung weight/body weight ratio were collected and measured on gestational days 19.5, 20.5 and 21.5. Tracheal samples were histologically processed, and epithelial, chondral and total tracheal thicknesses were measured on each gestational day. RESULTS: The tracheal occlusion group exhibited an increase in total lung weight/body weight ratio (p<0.001). Histologically, this group had a thicker epithelial thickness (p<0.05) and thinner chondral (p<0.05) and total tracheal thicknesses (p<0.001). These differences were more prominent on gestational days 20.5 and 21.5. CONCLUSION: Tracheal occlusion changed tracheal morphology, increased epithelial thickness and considerably decreased total tracheal thickness. These changes in the tracheal wall could explain the development of tracheomegaly, recently reported in some human fetuses subjected to tracheal occlusion.
dc.description59
dc.description63
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherFaculdade de Medicina / USP
dc.relationClinics
dc.rightsaberto
dc.sourceSciELO
dc.subjectTherapeutic Occlusion
dc.subjectTrachea
dc.subjectHernia
dc.subjectDiaphragmatic
dc.subjectRats
dc.subjectExperimental Model
dc.titleEvaluation of histological changes after tracheal occlusion at different gestational ages in a fetal rat model
dc.typeArtículos de revistas


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