Atherosclerosis in aged mice over-expressing the reverse cholesterol transport genes

dc.creatorBerti, J.A.
dc.creatorde Faria, E.C.
dc.creatorOliveira, H.C.F.
dc.date2005-03-01
dc.date2014-07-18T13:28:40Z
dc.date2015-11-26T11:39:35Z
dc.date2014-07-18T13:28:40Z
dc.date2015-11-26T11:39:35Z
dc.date.accessioned2018-03-28T20:42:58Z
dc.date.available2018-03-28T20:42:58Z
dc.identifierBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 3, p. 391-398, 2005.
dc.identifier0100-879X
dc.identifierS0100-879X2005000300010
dc.identifier10.1590/S0100-879X2005000300010
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2005000300010
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300010
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/31209
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/31209
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1234248
dc.descriptionWe determined whether over-expression of one of the three genes involved in reverse cholesterol transport, apolipoprotein (apo) AI, lecithin-cholesterol acyl transferase (LCAT) and cholesteryl ester transfer protein (CETP), or of their combinations influenced the development of diet-induced atherosclerosis. Eight genotypic groups of mice were studied (AI, LCAT, CETP, LCAT/AI, CETP/AI, LCAT/CETP, LCAT/AI/CETP, and non-transgenic) after four months on an atherogenic diet. The extent of atherosclerosis was assessed by morphometric analysis of lipid-stained areas in the aortic roots. The relative influence (R²) of genotype, sex, total cholesterol, and its main sub-fraction levels on atherosclerotic lesion size was determined by multiple linear regression analysis. Whereas apo AI (R² = 0.22, P < 0.001) and CETP (R² = 0.13, P < 0.01) expression reduced lesion size, the LCAT (R² = 0.16, P < 0.005) and LCAT/AI (R² = 0.13, P < 0.003) genotypes had the opposite effect. Logistic regression analysis revealed that the risk of developing atherosclerotic lesions greater than the 50th percentile was 4.3-fold lower for the apo AI transgenic mice than for non-transgenic mice, and was 3.0-fold lower for male than for female mice. These results show that apo AI overexpression decreased the risk of developing large atherosclerotic lesions but was not sufficient to reduce the atherogenic effect of LCAT when both transgenes were co-expressed. On the other hand, CETP expression was sufficient to eliminate the deleterious effect of LCAT and LCAT/AI overexpression. Therefore, increasing each step of the reverse cholesterol transport per se does not necessarily imply protection against atherosclerosis while CETP expression can change specific athero genic scenarios.
dc.description391
dc.description398
dc.languageen
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relationBrazilian Journal of Medical and Biological Research
dc.rightsaberto
dc.sourceSciELO
dc.subjectApolipoprotein AI
dc.subjectCholesteryl ester transfer protein
dc.subjectLecithin-cholesterol acyl transferase
dc.subjectAtherosclerosis
dc.subjectTransgenic mice
dc.titleAtherosclerosis in aged mice over-expressing the reverse cholesterol transport genes
dc.typeArtículos de revistas


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