Kinetics of TNF-alpha and IFN-gamma mRNA expression in islets and spleen of NOD mice

dc.creatorVentura-Oliveira, D.
dc.creatorVilella, C.A.
dc.creatorZanin, M.E.
dc.creatorCastro, G.M.
dc.creatorMoreira Filho, D.C.
dc.creatorZollner, R.L.
dc.date2002-11-01
dc.date2014-07-17T15:05:03Z
dc.date2015-11-26T11:25:30Z
dc.date2014-07-17T15:05:03Z
dc.date2015-11-26T11:25:30Z
dc.date.accessioned2018-03-28T20:39:28Z
dc.date.available2018-03-28T20:39:28Z
dc.identifierBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 11, p. 1347-1355, 2002.
dc.identifier0100-879X
dc.identifierS0100-879X2002001100013
dc.identifier10.1590/S0100-879X2002001100013
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2002001100013
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100013
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/24201
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/24201
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1233358
dc.descriptionInsulin-dependent diabetes mellitus is caused by autoimmune destruction of pancreatic ß cells. Non-obese diabetic (NOD) mice spontaneously develop diabetes similar to the human disease. Cytokines produced by islet-infiltrating mononuclear cells may be directly cytotoxic and can be involved in islet destruction coordinated by CD4+ and CD8+ cells. We utilized a semiquantitative RT-PCR assay to analyze in vitro the mRNA expression of TNF-alpha and IFN-gamma cytokine genes in isolated islets (N = 100) and spleen cells (5 x 10(5) cells) from female NOD mice during the development of diabetes and from female CBA-j mice as a related control strain that does not develop diabetes. Cytokine mRNAs were measured at 2, 4, 8, 14 and 28 weeks of age from the onset of insulitis to the development of overt diabetes. An increase in IFN-gamma expression in islets was observed for females aged 28 weeks (149 ± 29 arbitrary units (AU), P<0.05, Student t-test) with advanced destructive insulitis when compared with CBA-j mice, while TNF-alpha was expressed in both NOD and CBA-j female islets at the same level at all ages studied. In contrast, TNF-alpha in spleen was expressed at higher levels in NOD females at 14 weeks (99 ± 8 AU, P<0.05) and 28 weeks (144 ± 17 AU, P<0.05) of age when compared to CBA-j mice. The data suggest that IFN-gamma and TNF-alpha expression in pancreatic islets of female NOD mice is associated with ß cell destruction and overt diabetes.
dc.description1347
dc.description1355
dc.languageen
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relationBrazilian Journal of Medical and Biological Research
dc.rightsaberto
dc.sourceSciELO
dc.subjectDiabetes
dc.subjectNOD mice
dc.subjectCytokines
dc.subjectRT-PCR
dc.subjectPancreatic islets
dc.subjectTime course
dc.titleKinetics of TNF-alpha and IFN-gamma mRNA expression in islets and spleen of NOD mice
dc.typeArtículos de revistas


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