dc.contributor | Vinueza, Diego | |
dc.contributor | Escudero, Elizabeth | |
dc.creator | Portero Herrera, Santiago Andrés | |
dc.date.accessioned | 2018-01-24T20:14:37Z | |
dc.date.available | 2018-01-24T20:14:37Z | |
dc.date.created | 2018-01-24T20:14:37Z | |
dc.date.issued | 2017-11 | |
dc.identifier | Portero Herrera, Santiago Andrés. (2017). Evaluación de la actividad antiinflamatoria y citotóxica in vitro de Crinum x amabile. Escuela Superior Politécnica de Chimborazo. Riobamba. | |
dc.identifier | http://dspace.espoch.edu.ec/handle/123456789/7937 | |
dc.description.abstract | The objective of this research was to evaluate the anti-inflammatory and cytotoxic activities in vitro of crude and alkaloidal extracts of Crinum x amabile. The Crinum x amabile vegetal species was collected in Muisne canton located west of the province of Esmeraldas in Ecuador, approximately at the geographic coordinates: 0°36’09.5” N 80°01’30.1” W. Crinum x amabile crude extracts, using as extractive solvent methanol. On the other hand, the leaf and bulb alkaloid extracts of the study were obtained by liquid-liquid extraction using organic solvents. In vitro anti-inflammatory and cytotoxic activities were evaluated using the isolated neutrophil model, which is based on the reduction of a tetrazolium salt (WST-1). The antioxidant activity was developed by the DPPH free radical scavenging method. The results obtained from de antiinflammaory activity assay show that Crinum x amabile crude bulb extract at a concentration of 200 ppm possesses a market anti-inflammatory activity with a 56.35% ±0.24 of inflammatory inhibition. However, the value obtained is lower than that shown by the reference substance (acetylsalicylic acid), 70.28%±0.24 of inflammatory inhibition. The obtained results of cytotoxic activity demonstrate that the alkaloidal extract of the bulb of Crinum x amabile at a concentration of 12.5 ppm presents greater cellular viability with a 65.75%±0.31. | |
dc.language | spa | |
dc.publisher | Escuela Superior Politécnica de Chimborazo | |
dc.relation | UDCTFC;56T00745 | |
dc.subject | BIOQUÍMICA | |
dc.subject | FARMACIA | |
dc.subject | BULBO – HOJAS (Crinum x amabile) | |
dc.subject | AMARYLLIDACEAE | |
dc.subject | ACTIVIDAD ANTIINFLAMATORIA | |
dc.subject | ACTIVIDAD CITOTÓXICA | |
dc.subject | SAL DE TETRAZOLIO (WST-1) | |
dc.subject | NEUTRÓFILOS AISLADOS | |
dc.title | Evaluación de la actividad antiinflamatoria y citotóxica in vitro de Crinum x amabile. | |
dc.type | Tesis | |