Determinación de la Actividad Antimicrobiana del Extracto Etanólico y Subextractos Etéreo y Clorofórmico de (Drymaria ovatta), (Senna macrophylla) y (Tagetes filifolia Lag)

Abstract

The antimicrobial activity of the ethanolic extract and ethanolic and chloroformic subextracts of fish scale (Drymaria ovata), yellow flower (Senna macrophylla) and anisette (Tagetes filifolia Lag) was determined. Ethanolic extracts were obtained by maceration and concentrated at rotavapor. Sub-extracts were extracted by immiscibility using separating funnels and solvents such as ether and chloroform. The presence of Quinones, Phenols, Tannins, Flavonoids, Lactones, Saponins, Triterpenes and Steroids, Chalcones, Flavones, Lipids and essential oils was verified by coloring and precipitation reactions. The antimicrobial activity of the extracts and subextracts against Staphylococcus aureus ATCC 6538, Salmonella gallinarum ATCC 9184, Klebsiella pneumoniae ATCC 10031, Candida albicans ATCC 10231, Escherichia coli ATCC 9637 and Pseudomonas aeruginosa ATCC 27853 was determined by applying Mitscher's method. The ethanolic extract and the ethereal and chloroformic subextracts of Drymaria ovata is active for Candida albicans ATCC 10231, at a concentration of 10,000 µg/mL. Chloroformic Subextract of Senna macrophylla is partially active against Candida albicans ATCC 10231 at a concentration of 10,000 µg/mL. The ethanolic extract of Tagetes filifolia Lag shows partial activity against Candida albicans ATCC 10231 at a concentration of 10,000 µg/mL. The Ethanolic subextract at a concentration of 10,000 µg/mL is active against Staphylococcus aureus ATCC 6538 and Candida albicans ATCC 10231. The chloroformic subextract shows partial activity against Staphylococcus aureus ATCC 6538 and Candida albicans ATCC 10231 at a concentration of 10,000 µg/mL. It was found that the ethanolic extracts and ethereal and chloroformic subextracts of the studied plants present antimicrobial activity, it is recommended to continue the studies to determine other pharmacological activity.