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Inorganic pyrophosphate-phosphohydrolytic activity associated with rat osseous plate alkaline phosphatase
(Cellular & Molecular Biology, 1998-03-01)
Purified membrane-bound alkaline phosphatase from rat osseous plate hydrolyzed pyrophosphate in the presence of magnesium ions, with a specific activity of 92.7 U/mg. Optimal apparent pH for pyrophosphatase activity was ...
ALLOSTERIC MODULATION BY ATP, CALCIUM AND MAGNESIUM-IONS OF RAT OSSEOUS PLATE ALKALINE-PHOSPHATASE
(Elsevier B.V., 1993-09-03)
Alkaline phosphatase from rat osseous plate is allosterically modulated by ATP, calcium and magnesium at pH 7.5. At pH 9.4, the hydrolysis of ATP and PNPP follows Michaelis-Menten kinetics with K0.5 values of 154 muM and ...
Inorganic pyrophosphate-phosphohydrolytic activity associated with rat osseous plate alkaline phosphatase
(Cellular & Molecular Biology, 1998-03-01)
Purified membrane-bound alkaline phosphatase from rat osseous plate hydrolyzed pyrophosphate in the presence of magnesium ions, with a specific activity of 92.7 U/mg. Optimal apparent pH for pyrophosphatase activity was ...
Allosteric modulation of pyrophosphatase activity of rat osseous plate alkaline phosphatase by magnesium ions
(Elsevier B.V., 1998-01-01)
Pyrophosphatase activity of rat osseous plate alkaline phosphatase was studied at different concentrations of calcium and magnesium ions. with the aim of characterizing the modulation of enzyme activity by these metals. ...
ALLOSTERIC MODULATION BY ATP, CALCIUM AND MAGNESIUM-IONS OF RAT OSSEOUS PLATE ALKALINE-PHOSPHATASE
(Elsevier B.V., 1993-09-03)
Alkaline phosphatase from rat osseous plate is allosterically modulated by ATP, calcium and magnesium at pH 7.5. At pH 9.4, the hydrolysis of ATP and PNPP follows Michaelis-Menten kinetics with K0.5 values of 154 muM and ...
Quantum-mechanical study on the catalytic mechanism of alkaline phosphatases
(American Chemical Society, 2017-02-13)
Alkaline phosphatases (APs) catalyze the hydrolysis and transphosphorylation of phosphate monoesters. The catalytic mechanism was examined by quantum-mechanical calculations using an active-site model based on the X-ray ...