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rpb2 is a reliable reference gene for quantitative gene expression analysis in the dermatophyte Trichophyton rubrum
(INFORMA HEALTHCARELONDON, 2012)
The selection of reference genes used for data normalization to quantify gene expression by real-time PCR amplifications (qRT-PCR) is crucial for the accuracy of this technique. In spite of this, little information regarding ...
Selection and validation of reference genes by RT-qPCR under photoperiodic induction of flowering in sugarcane (Saccharum spp.)
(2021-12-01)
Although reference genes have previously been used in the expression analysis of genes involved in sugarcane flowering they had not been experimentally validated for stability and consistency of expression between different ...
Identification and evaluation of antioxidant and reference genes for quantitative real-time PCR in blood of Caiman latirostris
(Elsevier Science, 2021-02)
The quantitative real-time polymerase chain reaction (qPCR) has been one of the most promising approaches to perform rapid and accurate quantification of DNA in various biological systems. The aim of this study was to ...
Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions
(Genetics and Molecular Research, 2017)
Selection and validation of reference genes for quantitative gene expression analyses in various tissues and seeds at different developmental stages in Bixa orellana L.
(Physiology and Molecular Biology of Plants, 2018)
Identification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus
(Springer, 2018-10-01)
Due to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune ...
Selection of suitable reference genes for gene expression analysis in gills and liver of fish under field pollution conditions
(Nature Publishing Group, 2019)
To understand the role of gene expression in adaptive variation, it is necessary to examine expression variation in an ecological context. Quantitative real-time PCR (qPCR) is considered the most accurate and reliable ...
Validation of reference transcripts in strawberry (Fragaria spp.)
(Springer Heidelberg, 2013-12)
A set of strawberry (Fragaria spp.) constitutively-expressed reference genes has been identified by merging digital gene expression data with expression profiling. Constitutive reference candidates were validated using ...