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Identificación molecular por PCR y nested PCR de rickettsias en Crassostrea gigas.
(Universidad Nacional de Tumbes, 2012)
La presente investigación se realizó del 5 de diciembre del 2011 al 20 de marzo del 2012 en el Laboratorio de Biología Molecular de la Facultad de Ingeniería Pesquera de la Universidad Nacional de Tumbes. Se pretendió ...
A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers
(Blackwell Publishing, 2004-01-01)
Aims: Detection of Xylella fastidiosa in citrus plants and insect vectors.Methods and Results: Chelex 100 resin matrix was successfully standardized allowing a fast DNA extraction of X. fastidiosa. An amplicon of 500 bp ...
A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers
(Blackwell Publishing, 2004-01-01)
Aims: Detection of Xylella fastidiosa in citrus plants and insect vectors.Methods and Results: Chelex 100 resin matrix was successfully standardized allowing a fast DNA extraction of X. fastidiosa. An amplicon of 500 bp ...
Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams
(Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária, 2014)
Validation of a one-tube nested real-time PCR assay for the detection of Cryptosporidium spp. in avian fecal samples
(2022-01-01)
The aim of this study was to validate a one-tube nested real-time PCR assay followed by genetic sequencing to detect and identify Cryptosporidium species and genotypes in birds. A total of 443 genomic DNA extracted from ...
Detection of Mycoplasma hyopneumoniae by polymerase chain reaction in swine presenting respiratory problems
(Sociedade Brasileira de Microbiologia, 2008-09-01)
A Nested-PCR (N-PCR) tem como objetivo melhorar a sensibilidade do diagnóstico direto da Pneumonia Enzoótica Suína, pois o isolamento do Mycoplasma hyopneumoniae é trabalhoso tornando-se inviável na rotina. Neste trabalho, ...
Detection of Mycoplasma hyopneumoniae by polymerase chain reaction in swine presenting respiratory problems
(Sociedade Brasileira de Microbiologia, 2008-09-01)
A Nested-PCR (N-PCR) tem como objetivo melhorar a sensibilidade do diagnóstico direto da Pneumonia Enzoótica Suína, pois o isolamento do Mycoplasma hyopneumoniae é trabalhoso tornando-se inviável na rotina. Neste trabalho, ...