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Revealing a new fluorescence peak of the enhanced green fluorescent protein using three-dimensional fluorescence spectroscopy
(Royal Soc Chemistry, 2019-08-01)
Fluorescent proteins have many applications as biomarkers and biosensors in the medical and biological fields. Their success was largely supported by modifications of the first isolated fluorescent protein, the wild-type ...
Improving the cost effectiveness of enhanced green fluorescent protein production using recombinant Escherichia coli BL21 (DE3): Decreasing the expression inducer concentration
(2019-01-01)
Green fluorescent protein (GFP) is a globular protein used as biosensor and biomarker in medical and industrial fields. However, due to the expensive production costs of expressing proteins using high-cost inducers like ...
Aqueous Biphasic Systems Composed of Cholinium Chloride and Polymers as Effective Platforms for the Purification of Recombinant Green Fluorescent Protein
(2018-07-02)
Green fluorescent protein (GFP) has excellent properties as a biosensor and biomarker; however, its widespread use is limited by its purification costs. Alternative low-cost purification techniques can overcome this issue. ...
Protein refolding at high pressure: Optimization using eGFP as a model
(Elsevier Sci LtdOxfordInglaterra, 2011)
Violet ZnSe/ZnS as an alternative to green CdSe/ZnS in nanocrystal- fluorescent protein FRET systems
(American Chemical Society, 2009-06)
Fluorescent proteins from the green fluorescent protein family strongly interact with CdSe/ZnS and ZnSe/ZnS nanocrystals at neutral pH. Green emitting CdSe/ZnS nanocrystals and red emitting fluorescent protein dTomato ...
Extraction of recombinant proteins from Escherichia coli by cell disruption with aqueous solutions of surface-active compounds
(2018-07-01)
BACKGROUND: Green fluorescent protein (GFP) is used extensively as a biomarker due to its unique spectral and fluorescence characteristics. GFP is usually obtained from recombinant strains of Escherichia coli (E. coli) ...
Use of In Vivo and In Vitro Systems to Select Leishmania amazonensis Expressing Green Fluorescent Protein
(Korean Soc Parasitology, Seoul Natl Univ Coll Medi, 2011-12-01)
Various Leishmania species were engineered with green fluorescent protein (GFP) using episomal vectors that encoded an antibiotic resistance gene, such as aminoglycoside geneticin sulphate (G418). Most reports of GFP-Leishmania ...
Use of In Vivo and In Vitro Systems to Select Leishmania amazonensis Expressing Green Fluorescent Protein
(Korean Soc Parasitology, Seoul Natl Univ Coll Medi, 2011-12-01)
Various Leishmania species were engineered with green fluorescent protein (GFP) using episomal vectors that encoded an antibiotic resistance gene, such as aminoglycoside geneticin sulphate (G418). Most reports of GFP-Leishmania ...
Caspase-Cleaved Tau Impairs Mitochondrial Dynamics in Alzheimer’s Disease
(Humana Press Inc., 2018)
Use of In Vivo and In Vitro Systems to Select Leishmania amazonensis Expressing Green Fluorescent Protein
(Korean Soc Parasitology, Seoul Natl Univ Coll Medi, 2014)